FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54320307
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTTGCAGCAACAAGTTTTTTACCCTCCT754660.1389277862512817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT4150.020.954621
CGTATCG50100.016.1803653
CGGGGAT82450.016.1161211
CGCGTTT9650.015.8603671
CGCGGTT15750.015.4598531
GTATCGA50550.015.35394
CGGGTAT38050.014.5355121
GCGGGTT71050.014.3936551
CCGTATC67150.013.8699822
TCTATCG16100.013.71253862
CCCGTAT71900.013.3042891
CCGACTA17450.013.24502427
CTATCGC15900.013.23416463
CGGGCAA58800.012.7780411
CGGGCTA35650.012.4893061
ATCGCGC64150.011.99257167
CCGTTAA67100.011.9267721
CGGGATA28400.011.7581981
GCCGTTA68650.011.75797820
CGGGTTA26400.011.7266491
Produced by FastQC (version 0.11.5)