FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54320307
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAACTTGTTGCTGCAAGTCAAGCTGCCTTAGGCTTATAACATCACATTTAAAAGCATCTCAGCCTACCATGA571920.10528659199219915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT27950.022.8408471
GCGCTAA67550.016.2406642
ACCGTTG37900.016.2025413
CCGTTGA41850.014.9207624
CGCTAAG75900.014.2728243
CCGTATG59300.012.91715811
CGCGGGT55550.012.6726781
CGTATGG61700.012.58247512
CCGTCAT96100.012.3884791
TACCGTA85400.012.0400179
TACTCTA107450.011.463859
GCGCGTA7250.011.4204855
TCGATCG115000.011.33938469
GCGCGAA60600.011.27205958
CGGTAAG18750.011.22407344
CGCGAAC63750.010.82304259
TTTCGTC121300.010.8087950
CCTTAGG414550.010.66945529
GTTTCGT123100.010.62271449
CTTAGGC416950.010.56631730
Produced by FastQC (version 0.11.5)