FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55315014
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTG108200.022.3197638
CGCCGTA37700.019.02327558
GCCGTAT41050.017.63942359
CTCTAGT143450.017.4622786
CGACCGT45800.017.1947921
CGCGGGT107600.016.3071841
TGTACTC155500.016.2415682
TACTCTA171000.016.0473484
TAGTTGT158500.015.8923899
CGTATCA45600.015.80373361
CTCTAAT161600.015.36351519
GCGCTAA94000.015.25979942
TCTAATG161200.015.18772620
TCTAGTT179900.015.0034237
CCGTTGA52900.014.8022244
GTTACCT167100.014.40295914
CCGTCAT106550.014.3607731
ACTCTAG185350.014.318565
CGCTAAG102150.014.31233343
ACCGTTG53650.014.141263
Produced by FastQC (version 0.11.5)