FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004693281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59359217
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCGAATCTCGGTT3005420.5063105869472638TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCGAATCTCGTTT2213910.3729681946444813TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCGAATCTCGTAT2142770.36098353521071547TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCGAATCTCGGAT2049330.3452420876778075TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCGAATCTCGGGT1283580.2162393752599533TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCGAATCTCGTGT625940.10544950416040696TruSeq Adapter, Index 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)