FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004693311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65311983
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGAATCTCGTAT7178811.0991566432763187TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGAATCTCGGAT3194910.4891766951862417TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGAATCTCGTTT3050270.4670306825624939TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGAATCTCGGTT2932700.4490293917427067TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGAATCTCGGGT1268130.1941649819451968TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGAATCGCGTAT857890.13135261870704493TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGAATCTCGTGT780670.11952936722193844TruSeq Adapter, Index 20 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)