FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004693335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37979129
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATTAACATCTCGTAT6514581.715305266742689TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATTAACATCGCGTAT2529450.6660105343648086TruSeq Adapter, Index 2 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT687270.1809599161686936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)