FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004693337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43070458
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTTATAATCTCGTAT8882462.0623091586349047TruSeq Adapter, Index 14 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTTATAATCGCGTAT3071910.7132290072234662TruSeq Adapter, Index 14 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1068910.24817706837480114No Hit
GGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTC556970.12931601516751923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)