FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004693345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39114930
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTATCTCGTAT8556672.1875713442411886TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTATCGCGTAT3650230.9332063230076086TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT573300.1465680751569797No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT542170.13860947725075823No Hit
GGCAAAATGATTAAAGAAATTTATGTTTTTTAGACAGGGTGTTGGCTTTA539240.13786040266466026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)