FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004693353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42222405
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGATATCTCGTAT6689441.5843341941322384TruSeq Adapter, Index 25 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGATATCGCGTAT2054270.48653552539226513TruSeq Adapter, Index 25 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCAGATATCTCGTAT809420.1917039069659817TruSeq Adapter, Index 13 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770030.18237473682515243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)