FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004717341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11272354
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1481731.3144814295221745No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG860900.7637269021182266No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG552240.4899065448086531No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT534640.47429312457717354No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG479830.4256697403222077No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG291510.25860614384537606No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC264970.2350618158372244No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC222410.1973057269138283No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG205350.18217135480308727No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA185270.16435786172080827No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA182890.16224650148496048No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG180890.16047224918592867No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT174790.15506077967388177No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT166330.14755569244897737No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC156380.1387287872612943No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA153470.13614725016620308No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG151310.13423105768324878No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC150540.13354797054812154No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG148360.1316140355421769No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA146170.1296712292747371No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT145520.1290945972775518No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT142730.12661951532040247No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC132560.1175974423798259No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG131290.11647079216994072No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC125700.11151175699414691No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT120750.10712048255404327No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA120060.1065083655108773No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC119980.10643739541891606No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG117520.10425506509110698No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG116450.10330584011112498No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT116420.10327922632663948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT13650.060.358041
ACCGTTG16750.049.016263
GCGATTT42500.044.29218394
CGCAAAA32200.044.0749131
CGGTGTG275700.042.9936371
TCACCCG51700.038.929933
CACCCGG52250.037.989674
GACCGTT21950.036.9785352
TCAGACG118750.035.4007572
GGTGTGA377150.032.7796752
ATCAGAC126600.031.6572881
GTCTAAC44200.030.1514034
GTAGGTC33950.029.9887312
GTGTGAG638800.029.9885831
GTTGACT55000.028.99311
TGTGAGG677700.028.9524062
CTCGATT40950.028.77079848-49
CCGTTGA28800.028.6768634
CGGACAC42100.028.1590212-13
CATCTAA671050.027.28232694
Produced by FastQC (version 0.11.5)