FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004717345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11240799
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1504801.338694873914212No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG930930.8281706665157876No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG593010.5275514667596138No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT565430.5030158443363323No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG499600.44445239168496825No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG304390.2707903592974129No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC301950.2686196950946281No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC248620.22117644840015377No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG215630.19182800083873042No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA209360.18625010553075452No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA208890.18583198578677546No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG208880.18582308962200997No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT193590.17222085369554246No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT189610.1686801801188688No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC172420.153387672886954No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA170850.15199097501876868No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA170490.15167071308721025No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG169870.15111915087174854No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG169550.15083447359925214No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC166930.14850367843068807No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT164610.14643976820508933No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT157720.14031031068165173No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC152500.13566651267405458No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG150780.13413637233438655No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC144830.12884315429890705No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGGCTTCCCGGA136330.12128141424822203No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG136090.12106790629384977No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT135010.12010712049917449No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA134740.11986692405050567No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC133070.1183812645346652No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT131970.1174026864104589No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG122890.10932496880337421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT12700.057.4747471
GCGATTT41000.045.83082694
ACCGTTG16100.045.8057863
CGGTGTG292900.041.3109661
CGCAAAA38750.040.2095071
TCACCCG53750.039.608043
CACCCGG56700.036.5736584
GACCGTT20500.035.838422
TCAGACG120050.033.5227282
GGTGTGA382100.031.3765472
GTCTAAC48750.030.357084
ATCAGAC130400.030.2127821
TAACCCC53150.029.6696999
GTAGGTC31950.029.4393562
GTGTGAG660300.029.2731951
TTAACCC54800.028.268678
TGTGAGG681750.028.0532072
GAGTCTA53700.027.885522
TCACATG58750.027.7984522
AGTAGGT34550.027.3564381
Produced by FastQC (version 0.11.5)