FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975672
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33450.3428406267680122No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT30550.3131175231020261No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG29090.29815347780811585No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACC24800.25418378307463985No Hit
GTCTCCAGACACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGC22490.23050779360276816No Hit
CAATAATACACAGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATA21810.22353823826039898No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC21060.21585122869160947No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20850.2136988660123484No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG20520.21031658180208102No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20070.20570437606080733No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19110.19586500381275676No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGACT18930.19402012151624726No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT18610.1907403307668971No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA17760.18202838658893564No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG17730.18172090620618406No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG17510.1794660500660058No Hit
ATAATACACAGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATATAGTGAGTTCTTGGCGTTGTCTCTGG16980.17403389663739455No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16790.17208652087996784No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16190.16593691322493626No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT15810.1620421617100829No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTG15380.1576349428906436No Hit
GTCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGAT14930.15302273714936987No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG14890.15261276330570112No Hit
GTATTATTGTGTGAGGAATGAGGCAAGTGGGAACTTCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCGC14410.14769307718167582No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGACTTTGCAGTTTATTACTGTCAGGACTATAATAACTGG14380.1473855967989242No Hit
GTCTGAGGACTTTGCAGTTTATTACTGTCAGGACTATAATAACTGGCCTT14250.14605318180700072No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC14170.14523323411966316No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATC14140.14492575373691158No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG13760.14103100222205822No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGG13590.13928861338646595No Hit
CTCCAGACACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCA13460.13795619839454243No Hit
GTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCCCTTGGCCGAACGTCCAAGGCCAGTTATTATAGTCCTGAC13210.1353938618716126No Hit
GTTTATTACTGTCAGGACTATAATAACTGGCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGA12790.13108913651309048No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12760.13078165613033887No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12520.12832181306832624No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC12500.12811682614649186No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12280.1258619700063136No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA12260.12565698308447923No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT12170.12473454193622448No Hit
GTAATAAACTGCAAAGTCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCC12100.12401708770980412No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12020.12319714002246655No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC11770.12063480349953674No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11650.11940488196853041No Hit
CTCCTGTGCAGCCTCTGGAATCACTTTTAGTACCCATTGGTTGAGCTGGG11610.11899490812486163No Hit
GTTATTATAGTCCTGACAGTAATAAACTGCAAAGTCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT11540.11827745389844128No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCTGAAACCTGGCCAGGCTCCCAAGCT11400.11684254544560056No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11360.1164325716019318No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAAATGAAGAGCTTGGGAGCCTGGCCAGGTTTCAGCTGGTACCAGG11230.11510015661000829No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCCCTTGGCCGAACGTCCAAGGCCAGTT11150.11428020892267073No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC10960.11233283316524406No Hit
ATAATAACTGGCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAACTCAAA10730.1099754835641486No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGC10680.10946301625956265No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT10660.10925802933772824No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA10610.1087455620331423No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC10540.10802810780672194No Hit
GTGTATTATTGTGTGAGGAATGAGGCAAGTGGGAACTTCTTTGACTACTG10460.10720816011938437No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAG10440.10700317319754998No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC10410.10669569281479842No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10390.10649070589296404No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT10300.10556826474470929No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAG10180.10433834321370297No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT10150.10403086283095138No Hit
CTATAATAACTGGCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGAACTGTGGCTGCACCAT10050.10300592822177945No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC9820.10064857862068399No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTG9800.10044359169884962No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9790.10034109823793241No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)