FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975672
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33300.3413032248542543No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG29720.304610565845899No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT26490.2715051779696455No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACC23820.24413942390475485No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC21850.22394821210406776No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21280.21810608483178773No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20550.2106240621848326No Hit
GTCTCCAGACACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGC19940.20437196106888378No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19020.194942562664502No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGACT18880.19350765421166127No Hit
CAATAATACACAGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATA18630.19094531768873146No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG17590.18028599775334334No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17490.1792610631441714No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA17490.1792610631441714No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT17330.1776211677694963No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17000.17423888355922892No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG16970.17393140317647734No Hit
ATAATACACAGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATATAGTGAGTTCTTGGCGTTGTCTCTGG15210.15589255405505129No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT15020.1539451782976246No Hit
GTCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGAT14930.15302273714936987No Hit
GTCTGAGGACTTTGCAGTTTATTACTGTCAGGACTATAATAACTGGCCTT14510.14871801179084776No Hit
GTATTATTGTGTGAGGAATGAGGCAAGTGGGAACTTCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCGC14040.14390081912773967No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13880.14226092375306457No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG13660.1400060676128863No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC13620.13959609376921753No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATC13390.13723874416812207No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTG13320.1365212899417017No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13120.13447142072335785No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGACTTTGCAGTTTATTACTGTCAGGACTATAATAACTGG12860.13180659073951081No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGG12840.13160160381767644No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12530.12842430652924341No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG12330.12637443731089956No Hit
GTAATAAACTGCAAAGTCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCC12270.1257594765453964No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG12120.12422207463163851No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12080.12381210078796974No Hit
CTCCTGTGCAGCCTCTGGAATCACTTTTAGTACCCATTGGTTGAGCTGGG12060.12360711386613535No Hit
GTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCCCTTGGCCGAACGTCCAAGGCCAGTTATTATAGTCCTGAC12040.12340212694430094No Hit
GTTTATTACTGTCAGGACTATAATAACTGGCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGA11870.12165973810870866No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCTGAAACCTGGCCAGGCTCCCAAGCT11780.12073729696045392No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11590.11878992120302725No Hit
CTCCAGACACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCA11570.11858493428119286No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11470.11755999967202092No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC11210.1148951696881739No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA11190.11469018276633952No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT11160.11438270238358793No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC11040.1131527808525816No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10850.11120540509515493No Hit
GTTATTATAGTCCTGACAGTAATAAACTGCAAAGTCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT10760.1102829639469002No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10740.1100779770250658No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC10700.10966800318139704No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT10330.10587574512746087No Hit
CTCCAGAGACAACGCCAAGAACTCACTATATCTGCAAATGAATAGCCTGA10300.10556826474470929No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT10290.10546577128379209No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10240.10495330397920612No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCCCTTGGCCGAACGTCCAAGGCCAGTT10220.10474831705737174No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA10190.10444083667462017No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC10180.10433834321370297No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAAATGAAGAGCTTGGGAGCCTGGCCAGGTTTCAGCTGGTACCAGG10160.10413335629186858No Hit
GTGTATTATTGTGTGAGGAATGAGGCAAGTGGGAACTTCTTTGACTACTG10120.10372338244819981No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGC10060.10310842168269664No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC10050.10300592822177945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)