FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1274572
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41800.3279532266517701No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT39560.3103786996733021No Hit
CAGTTTACTCTCACCATAAGTAGCCTACAGCCTGAGGATGTTGCAACTTATTACTGTCAAAAGTATAACAGTGCC31920.25043700944316993No Hit
GTGATATAAGGCGCTGTCATCTGTTCTCAGACCGCTCATTTGCAGATACAGGGACTTTTTGCTGTTGTCTCTGG30400.23851143756492377No Hit
GTATAACAGTGCCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT29000.2275273582033812No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28050.22007387577947735No Hit
GAGTAAACTGTGTCCTAGATCCACTGCCACTGAACCGAGATGGGACCCCTGACTGCAAAGTGGTTGCACGAT27850.21850472158497125No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG25610.20093019460650322No Hit
TTATATCACTGTGTAAAAGATGTGGGCTACGATGACGACTATTTTGATTA25050.1965365628618862No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG23920.18767084166292686No Hit
CTGTTATACTTTTGACAGTAATAAGTTGCAACATCCTCAGGCTGTAGGCTACTTATGGTGAGAGTAAACTGTGTC23210.18210034427243027No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22890.17958969756122056No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA22460.1762160160430325No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT22210.17425457329989988No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22020.17276387681511912No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTT20340.15958298158126807No Hit
ATAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTTTCGATTATTTAGCCTGGTATCAGCAGA19840.15566009609500286No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18360.1440483550556579No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA18120.1421653700222506No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18100.1420084546028No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18000.14122387750554696No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG17890.1403608426985686No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC17820.1398116387304915No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17770.13941935018186496No Hit
CACCATAAGTAGCCTACAGCCTGAGGATGTTGCAACTTATTACTGTCAAAAGTATAACAGTGCCCCTCTCACTTT17640.13839939995543604No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATATCCGGTGTGACATCC17160.13463342988862145No Hit
ATCTAGGACACAGTTTACTCTCACCATAAGTAGCCTACAGCCTGAGGATG17070.1339273105010937No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16920.13275044485521414No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGGGGCACTGTTATACTTTTGAC16650.13063208669263093No Hit
GTTCTCAGACCGCTCATTTGCAGATACAGGGACTTTTTGCTGTTGTCTCTGGAGATGCTGAATCGGCCCTTCAC16280.1277291514327947No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15930.12498313159240906No Hit
GTCTGAGAACAGATGACAGCGCCTTATATCACTGTGTAAAAGATGTGGGCTACGATGACGACTATTTTGATT15920.12490467388268375No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATATCCGGTGTGACAT15180.11909880336301126No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC15120.11862805710465944No Hit
AAGTATAACAGTGCCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15080.11831422626575824No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15000.1176865645879558No Hit
GAGTTGGGTTTTCCTTGTTGCTATTTTAAAAGGAGTCCAGTGTGAAGTTC14960.11737273374905458No Hit
GTGCACAGAGAACTCACCATGGAGTTTGGACTGAGTTGGGTTTTCCTTGT14890.11682352978097746No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14800.11611741039344971No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCC14690.11525437558647138No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT14670.11509746016702077No Hit
GTTATACTTTTGACAGTAATAAGTTGCAACATCCTCAGGCTGTAGGCTACTTATGGTGAGAGTAAACTGTGTCCT14490.11368522139196531No Hit
CTCTAGAATGGATCGCTCTCATAAGTGGGGATGGGGATAGCGAAGTCTATCTGGACGCTGTGAAGGGCCGATTC14370.11274372887526167No Hit
GATCAGCTCTGTGCACAGAGAACTCACCATGGAGTTTGGACTGAGTTGGGTTTTCCTTGTTGCTATTTTAAAAGG14310.11227298261690982No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14310.11227298261690982No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGGGGC14170.11117457468075559No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13990.10976233590570011No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13820.10842855484036994No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13650.10709477377503977No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13570.10646711209723735No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13520.10607482354861084No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13510.10599636583888553No Hit
GTACAGGCGGGGGGGTCCCTGAGACTCTCCTGTGCAACCTCTGGATTCAG13500.10591790812916023No Hit
GTGTAAAAGATGTGGGCTACGATGACGACTATTTTGATTACTGGGGCCAGGGAACCCTGGTCTCCGTCTCCTC13400.10513333103190718No Hit
GTAATAAGTTGCAACATCCTCAGGCTGTAGGCTACTTATGGTGAGAGTAAACTGTGTCCTAGATCCACTGCC13150.10317188828877458No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC13000.10199502264289502No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCC12920.10136736096509259No Hit
GTCCTAGATCCACTGCCACTGAACCGAGATGGGACCCCTGACTGCAAAGTGGTTGCACGATAGATCAGGAGGCT12860.10089661470674077No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12850.10081815699701548No Hit
GGTATCAGCAGAAGCCAGGGAAACTTCCTAGCCTCCTGATCTATCGTGCAACCACTTTGCAGTCAGGGGTCCCAT12800.10042586844838895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)