FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1414639
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGGATAGTGGCCTTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC119680.8460108904109105No Hit
ATCCATATACAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATCGTGAGGGCAGCTTTGTT104080.7357354067009322No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC51520.3641918538934668No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAAGGCCACTATCCATATACAGCAC49420.3493470772402005No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAAGGCCACTATCCATATACAGCACACAGTAATAAT48810.3450350230694898No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG48570.34333847716625937No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGG44910.31746615214199525No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATCGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAG44310.3132247873839191No Hit
GATTATTACTGTGTGCTGTATATGGATAGTGGCCTTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGT43610.30827652849949705No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43110.304742057867767No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA42080.297461048366403No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGG39930.28226282464996366No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC37560.26550943385556314No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACAACACAAACATTCGCTCT35480.250806036027566No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35340.24981638425068164No Hit
TCCATATACAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATCGTGAGGGCAGCTTTGTTC34790.24592846655577855No Hit
CTACTAGTTACTACCCCAAATGGTACCGGCAGACCCCAGGCCAGGCTCCA34590.2445146783030865No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA33810.2390009041175876No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG31700.22408543805168668No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACGATCACGGGGGCCCAGG31420.22210613449791783No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT30740.21729925443876494No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC30360.21461305675865008No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT30340.2144716779333809No Hit
CCCCAGAAGAGCGAATGTTTGTGTTGTAGATGAGCGTGCGTGGAGCCTGG30200.21348202615649645No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30060.21249237437961205No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGG29890.21129065436482383No Hit
CTCATCTACAACACAAACATTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAA29080.2055648119414211No Hit
GTCTCTACTAGTTACTACCCCAAATGGTACCGGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAACACA28240.19962690128011457No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG27930.1974355294884419No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC26840.18973038351127036No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26660.18845797408384754No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG26310.1859838446416365No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC26200.18520626110265587No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC26170.18499419286475208No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26020.18393385167523305No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24810.17538043274644627No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG24700.17460284920746566No Hit
CTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAAATGGTACCGGCAGACCCCAGGCCAGGCTC24240.171351136226274No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA24120.17050286327465877No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24050.17000803738621656No Hit
ATACAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGA23800.16824080207035153No Hit
GGCCTGGGGTCTGCCGGTACCATTTGGGGTAGTAACTAGTAGAGACTGAG23680.1673925291187363No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAAATGGT23280.16456495261335224No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23100.16329254318592942No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT22470.1588391101899495No Hit
AGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTC22430.1585563525394111No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA22260.1573546325246229No Hit
CCCAAAGGCCACTATCCATATACAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATCGTG22200.1569304960488153No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG21980.15537532897085404No Hit
GTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCCGGTACCATTTGGGGTAGTAACTAGTAGAGACTGAGCC21980.15537532897085404No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCAC21810.15417360895606583No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT21500.15198223716439319No Hit
AACTAGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTC21460.15169947951385476No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21450.15162879010122018No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG21370.15106327480014337No Hit
ATAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATGCACTGAGTTCTTGGCGTTCTCTCTGG21190.14979086537272052No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC21110.14922535007164373No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG21090.1490839712463745No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG20820.14717535710524027No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC20680.14618570532835584No Hit
GCCTGGGCCCCCGTGATCGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATCAGGGACCCCAGA20590.14554950061464444No Hit
CATCTACAACACAAACATTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC20440.1444891594251254No Hit
CACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAAATGGTACCGGCAGACCCCAGGCCAGG20350.14385295471141402No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20310.14357019706087562No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20200.14279261352189498No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAAGGCCACTATCCATATACAGCACACAGTAATAATC19980.14123744644393377No Hit
GTATCAACGCAGAGTACGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTC19530.1380564228753767No Hit
CAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATCGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCC19530.1380564228753767No Hit
CTAGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGG19060.13473402048155042No Hit
GTGCTGTATATGGATAGTGGCCTTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGG18640.13176506515089717No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18620.13162368632562796No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT18540.13105817102455114No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC18450.13042196631083974No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG18260.1290788674707823No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAA18130.12815990510653247No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC18060.12766507921809028No Hit
CAATAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATG18040.12752370039282107No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17530.1239185403484564No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA17040.12045475912936092No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTC16360.11564787907020803No Hit
CTCTACTAGTTACTACCCCAAATGGTACCGGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAACACAA16280.11508236376913121No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT16220.1146582272933236No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC16160.11423409081751598No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA15710.11105306724895891No Hit
GTTTGGTCCCTCCGCCGAACACCCAAAGGCCACTATCCATATACAGCACACAGTAATAATCAGATTCATCATCTG15640.11055824136051672No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15520.10970996840890149No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG15450.10921514252045929No Hit
GTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAAATGGTACCGGCAGACCCC15440.1091444531078247No Hit
AGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTCCTGGGTCACCACAGTCTGAGAAT15420.10900307428255548No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15360.10857893780674788No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACC15350.10850824839411326No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15240.10773066485513265No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15060.10645825542770983No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCC15030.10624618718980602No Hit
TTATTACTGTGTGCTGTATATGGATAGTGGCCTTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTC14770.10440826246130637No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14610.10327723185915277No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAAGGCC14510.10257033773280674No Hit
ACATAGACCAAGGTGGAAGTGAGAAATACTATCTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGAGAACG14340.10136861771801853No Hit
ATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTT14170.1001668977032303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)