FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1414639
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGGATAGTGGCCTTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC116200.8214109748140692No Hit
ATCCATATACAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATCGTGAGGGCAGCTTTGTT98800.6984113968298626No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48500.34284365127781713No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGG46410.32806956403718546No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAAGGCCACTATCCATATACAGCAC43980.3108920367669773No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG43760.3093368696890161No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAAGGCCACTATCCATATACAGCACACAGTAATAAT43450.3071454978973434No Hit
GATTATTACTGTGTGCTGTATATGGATAGTGGCCTTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGT43140.3049541261056708No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41970.2966834648274224No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA41720.2949162295115574No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37880.26777149505987036No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGG37030.26176289498592925No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATCGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAG36750.25978359143216045No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACAACACAAACATTCGCTCT33990.24027331354501041No Hit
CTACTAGTTACTACCCCAAATGGTACCGGCAGACCCCAGGCCAGGCTCCA33270.2351836758353191No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32000.22620612043072474No Hit
TCCATATACAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATCGTGAGGGCAGCTTTGTTC32000.22620612043072474No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT31110.21991476270624521No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC30620.21645098148714972No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA30600.2163096026618805No Hit
GTCTCTACTAGTTACTACCCCAAATGGTACCGGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAACACA30070.21256306379224665No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACGATCACGGGGGCCCAGG29710.21001824493740098No Hit
CTCATCTACAACACAAACATTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAA29560.20895790374788198No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGG29460.20825100962153595No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG29110.2057768801793249No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT29040.20528205429088267No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC27980.19778897655161493No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC27840.19679932477473053No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG26990.1907907247007894No Hit
CCCCAGAAGAGCGAATGTTTGTGTTGTAGATGAGCGTGCGTGGAGCCTGG26810.18951831527336657No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG26230.18541832934055968No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26030.18400454108786765No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC25160.1778545621886573No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25060.1771476680623113No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC24800.17530974333381166No Hit
CTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAAATGGTACCGGCAGACCCCAGGCCAGGCTC24220.17120975740100478No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAAATGGT23340.16498908908915985No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22750.16081841374371839No Hit
AGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTC22390.15827359488887272No Hit
GGCCTGGGGTCTGCCGGTACCATTTGGGGTAGTAACTAGTAGAGACTGAG22380.1582029054762381No Hit
ATACAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGA22180.15678911722354608No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG21910.15488050308241183No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT21580.15254775246546998No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG21170.14964948654745133No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21030.1486598347705669No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20820.14717535710524027No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20710.14639777356625966No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG20630.14583225826518284No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCAC20590.14554950061464444No Hit
GTGCTGTATATGGATAGTGGCCTTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGG20530.14512536413883684No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC20350.14385295471141402No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA20330.1437115758861448No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG20300.143499507648241No Hit
ATAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATGCACTGAGTTCTTGGCGTTCTCTCTGG20110.1421564088081836No Hit
CCCAAAGGCCACTATCCATATACAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATCGTG19910.14074262055549153No Hit
AACTAGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTC19770.13975296877860713No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG19660.1389753852396265No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC19390.13706677109849227No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT19360.13685470286058848No Hit
GTATCAACGCAGAGTACGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTC19270.13621849814687703No Hit
GTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCCGGTACCATTTGGGGTAGTAACTAGTAGAGACTGAGCC19200.13572367225843485No Hit
CACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAAATGGTACCGGCAGACCCCAGGCCAGG18930.1338150581173006No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18790.13282540634041617No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA18570.13127023926245493No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18540.13105817102455114No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC18370.12985645100976292No Hit
CATCTACAACACAAACATTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC18300.12936162512132068No Hit
GCCTGGGCCCCCGTGATCGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATCAGGGACCCCAGA17830.12603922272749443No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17510.12377716152318718No Hit
CTAGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGG17360.12271682033366818No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17180.12144441090624533No Hit
CAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATCGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCC17180.12144441090624533No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAA17010.12024269089145712No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16900.1194651073524765No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16550.11699097791026544No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAAGGCCACTATCCATATACAGCACACAGTAATAATC16330.11543581083230423No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16020.11324443904063156No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTC15910.11246685550165095No Hit
TTATTACTGTGTGCTGTATATGGATAGTGGCCTTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTC15840.11197202961320875No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC15810.11175996137530494No Hit
CTCTACTAGTTACTACCCCAAATGGTACCGGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAACACAA15780.11154789313740114No Hit
CAATAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATG15690.1109116884236897No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15620.1104168625352475No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15570.1100634154720745No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG15300.10815480133094026No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA15020.10617549777717142No Hit
GGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGCTGTATATGGATA14560.10292378479597976No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACC14550.10285309538334514No Hit
GTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAAATGGTACCGGCAGACCCC14540.10278240597071055No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC14500.10249964832017215No Hit
AGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTCCTGGGTCACCACAGTCTGAGAAT14460.10221689066963373No Hit
ATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTT14310.10115654948011472No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG14270.1008737918295763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)