FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068706
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCGGACACTTTTGGCCAGGGGA47820.44745701811349425No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42530.3979579042318468No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT39800.37241299290918173No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC39700.3714772818717215No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC36100.33779168452315234No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28600.2676133567136331No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGT26970.25236126680303095No Hit
GTATGGTAGCTCACCGGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCAT26570.24861842265318992No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG26530.24824413823820585No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT26290.24599843174830122No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG25950.24281701422093635No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCAGATACTTAGCCTGGTACC25770.24113273435350788No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24730.2314013395639212No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC24610.23027848631896894No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGGTGCCAGTGGCCCTGCT23990.2244770778867153No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG23560.22045352042563623No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCGGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGA23140.21652353406830313No Hit
GGCCAAAAGTGTCCGGTGAGCTACCATACTGCTGACAGTAATACACTGCA23110.21624282075706508No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT22090.20669856817497048No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC22090.20669856817497048No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA21230.19865145325281228No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCACCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTC21030.19678003117789178No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAGC20910.19565717793293946No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20670.19341147144303483No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC20480.19163362047186036No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG19920.18639363866208294No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTATCTGCTACTAACACTCTG19750.1848029298984005No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC19720.18452221658716242No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA19290.18049865912608332No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC19210.17975009029611513No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC19020.1779722393249406No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18040.1688022711578301No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA17910.1675858468091318No Hit
TACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCC17770.16627585135668743No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17270.16159729616938615No Hit
GTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTCCGGTGAGCTACCATACTGCTGAC16530.15467303449218026No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTCCGGTGAGCT16440.15383089455846605No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGGCGGTGCTGATGGACTTGTCGGCTG16170.15130447475732334No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC15520.14522235301383168No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC14800.13848523354411782No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTC14700.13754952250665758No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC14370.13446167608303874No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14300.13380667835681656No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC14180.13268382511186425No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT14170.13259025400811825No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGGTGCCAGTGGCCCTGCTGGATGCACCAT13910.13015740531072156No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCC13850.1295959786882454No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13750.12866026765078514No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13650.1277245566133249No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC13530.1266017033683726No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC13500.1263209900571345No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13340.1248238523971981No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12880.12051958162488093No Hit
GTCAGAGTGTTAGTAGCAGATACTTAGCCTGGTACCAGCAGAAACCTGGC12820.11995815500240477No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12780.11958387058742068No Hit
CCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACC12780.11958387058742068No Hit
GTGTTAGTAGCAGATACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT12680.11864815954996041No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12590.11780601961624618No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAC12480.1167767374750399No Hit
ATGTATTACTGTGCGAGACGGGGGTTCGATGATTCGGGGAGTTATTATAGCCGCCGCGGTATGGACGTCTGGGGC12390.11593459754132569No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12290.11499888650386542No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT12010.11237889559897671No Hit
GGCTAAGTATCTGCTACTAACACTCTGACTGGCCCTGCAGGAGAGGGTGG11860.11097532904278631No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11790.11032033131656416No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG11790.11032033131656416No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTATCTGCTACTAACACTCTGACTGGCCCTGCAGG11580.1083553381378976No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCACCCCA11520.10779391151542145No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11330.10601606054424698No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11150.1043317806768185No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11050.10339606963935825No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCACCCCAGACAGGTT11000.10292821412062814No Hit
ACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCC10920.10217964529065991No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10890.10189893197942183No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10820.10124393425319966No Hit
CCTCATCTATGGTGCATCCAGCAGGGCCACTGGCACCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT10810.10115036314945364No Hit
CAGTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGGCGGTGCTGATGGACTTGTCGG10760.1006825076307235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)