FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068706
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCGGACACTTTTGGCCAGGGGA46510.43519920352276487No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42300.3958057688456882No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC37080.3469616526902628No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC34730.3249724433099468No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT32630.30532251152328144No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27250.2549812577079197No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT26030.24356558305090453No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCAGATACTTAGCCTGGTACC25220.2359863236474765No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25190.23570561033623838No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGT24870.2327113350163656No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG24290.2272842109990961No Hit
GTATGGTAGCTCACCGGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCAT24280.22719063989535007No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA24220.22662921327287391No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC23840.22307351133052494No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG23720.22195065808557263No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGGTGCCAGTGGCCCTGCT23390.2188628116619538No Hit
GGCCAAAAGTGTCCGGTGAGCTACCATACTGCTGACAGTAATACACTGCA22350.20913141687236714No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCGGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGA22280.20847641914614498No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC21000.19649931786665367No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAGC20710.19378575585801897No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT20590.19266290261306665No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC19760.18489650100214652No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG19720.18452221658716242No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19410.18162151237103563No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA18860.1764751016650042No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC18630.17432296627884564No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCACCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTC18500.1731065419301473No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTATCTGCTACTAACACTCTG18200.17029940881776653No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC17850.16702442018665564No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC17340.16225229389560833No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG17290.1617844383768782No Hit
TACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCC16600.15532803221840244No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16600.15532803221840244No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC16180.15139804586106936No Hit
GTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTCCGGTGAGCTACCATACTGCTGAC16150.15111733254983128No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCC15630.14625163515503797No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGGCGGTGCTGATGGACTTGTCGGCTG15380.14391235756138732No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTCCGGTGAGCT15370.1438187864576413No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15180.14204093548646682No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA15140.1416666510714827No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT15070.14101165334526053No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14910.13951451568532414No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC14770.13820452023287974No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC14690.13745595140291156No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14420.13492953160176888No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC14110.1320288273856421No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGGTGCCAGTGGCCCTGCTGGATGCACCAT13860.12968954979199143No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTC13450.12585313453840438No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13370.1251045657084362No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC13200.12351385694475375No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC13050.12211029038856336No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12760.11939672837992861No Hit
GTGTTAGTAGCAGATACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT12710.11892887286119849No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12560.11752530630500813No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT12410.11612173974881773No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12230.11443745988138926No Hit
GTCAGAGTGTTAGTAGCAGATACTTAGCCTGGTACCAGCAGAAACCTGGC11960.11191104008024659No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11830.11069461573154823No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG11520.10779391151542145No Hit
ATGTATTACTGTGCGAGACGGGGGTTCGATGATTCGGGGAGTTATTATAGCCGCCGCGGTATGGACGTCTGGGGC11470.10732605599669133No Hit
CCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACC11470.10732605599669133No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC11360.10629677385548504No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTATCTGCTACTAACACTCTGACTGGCCCTGCAGG11320.10592248944050094No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAC11150.1043317806768185No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11030.10320892743186622No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10950.10246035860189799No Hit
GAGTGTTAGTAGCAGATACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGC10940.10236678749815198No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10800.10105679204570762No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)