FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548145
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24100.4396646872634065No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21290.38840087932937456No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA21000.3831103084037982No Hit
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC18930.3453465780039953No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18660.3404208740388036No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT15690.286238130421695No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC15210.2774813233724653No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC14760.2692718167638125No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC14520.2648934132391977No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14200.2590555418730445No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13880.2532176705068914No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13490.24610276477939233No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAACTCTGTCTCCTGGT13400.24446086345766174No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCGAGTGCTGCGGCTTGTATATGAGCTGCAGTAATAAT13340.24336626257650804No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCGAGTGCTGCGGCTTGTATATGAGCTG13180.24044732689343148No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13170.2402648934132392No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG13140.23971759297266235No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG13080.23862299209150864No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC12810.23369728812631693No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12530.22858915068093297No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG12420.2265823823988178No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC12400.22621751543843324No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12090.22056207755247245No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11990.21873774275054958No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11900.21709584142881902No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11340.20687956653805106No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGATTACCAC11160.20359576389458994No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10980.2003119612511288No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCC10960.19994709429074423No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10660.1944740898849757No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10460.19082542028113No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCT10450.19064298680093772No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGATTACCACCTGATTACCACTGCTGTCTGTTGAGTAACAGTAGT9940.18133887931113118No Hit
GATTATTACTGCAGCTCATATACAAGCCGCAGCACTCGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCTAGGT9830.17933211102901606No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC9740.1776902097072855No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9620.17550100794497808No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAACTCTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC9610.17531857446478577No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA9470.1727645057420938No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9420.17185233834113237No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC9130.16656176741555612No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA9040.16491986609382556No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8810.160723896049403No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC8660.15798739384651872No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT8650.15780496036632644No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8490.15488602468324986No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8440.15397385728228846No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAACTCTGTCTCCTGGTACCAACAGC8250.15050762115863503No Hit
CCCGAGTGCTGCGGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTC8230.15014275419825046No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT7930.14466974979248193No Hit
CCCCTGTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCT7860.14339271543113594No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC7700.14047377974805936No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7590.13846701146594423No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT7480.1364602431838291No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG7450.13591294274325225No Hit
TCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC7440.13573050926305996No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC7370.13445347490171397No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAG7360.1342710414215217No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC7290.13299400706017567No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG7290.13299400706017567No Hit
GAGTTATAACCACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAT7060.12879803701575313No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA7040.12843317005536856No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC7040.12843317005536856No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC6910.12606153481286886No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA6890.1256966678524843No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC6760.12332503260998459No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC6760.12332503260998459No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG6690.12204799824863859No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC6650.12131826432786946No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTT6540.11931149604575432No Hit
GTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGGACA6490.11839932864479288No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC6350.11584525992210092No Hit
CTACTACTGTTACTCAACAGACAGCAGTGGTAATCAGGTGGTAATCGGCG6330.11548039296171635No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAG6180.11274389075883207No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA6050.11037225551633237No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC6030.11000738855594777No Hit
GACTACTACTGTTACTCAACAGACAGCAGTGGTAATCAGGTGGTAATCGGCGGAGGGACCAAGCTGACCGTCCT6010.10964252159556322No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG6000.10946008811537093No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG5980.10909522115498636No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGG5930.10818305375402493No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG5900.10763575331344807No Hit
GTTATAACTCTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAA5900.10763575331344807No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT5900.10763575331344807No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC5880.10727088635306352No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC5790.10562898503133294No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTAC5790.10562898503133294No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC5740.10471681763037154No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG5650.10307491630864096No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC5550.10125058150671812No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT5500.10033841410575668No Hit
CAGTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCC5490.1001559806255644No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)