FastQCFastQC Report
Wed 8 May 2024
EGAF00004717475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310117
Total Bases96.4 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGGTAGCTCACCTCAAGGTTTCGGCGGAGGGA59760.456142466665191No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGATCAGCTTCAGGGAGA54610.41683300041141363No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT43220.3298942002889818No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39990.3052399136870982No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC36890.28157790487414486No Hit
GTCTCTGGTGGCTCCGTCAGAAGTGGTAGTTACTACTGGAGCTGGATCCG35300.26944158422492037No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT33940.25906083197149565No Hit
CCTCATATATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCA33140.25295450711654No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30060.22944515642496052No Hit
GTGTATTACTGTGCGAGAGTCGGATGGAGTGGTGGCTACGTCGGGGTCAA28170.21501896395512768No Hit
GTATGGTAGCTCACCTCAAGGTTTCGGCGGAGGGACCAAGGTGGAGATCA27500.20990491688910226No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCT27150.20723339976505914No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26940.20563048949063328No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24780.18914341238225288No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24420.18639556619752282No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT24400.1862429080761489No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23940.1827317712845494No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT23860.1821211387990538No Hit
CATATATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTG23450.17899164731088904No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT23340.1781520276433326No Hit
CACCTGCACTGTCTCTGGTGGCTCCGTCAGAAGTGGTAGTTACTACTGGA23230.17731240797577622No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG22690.17319063869868112No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22460.17143507030288135No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22320.1703664634532641No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22320.1703664634532641No Hit
CAGTAATACACGGCCGTGTCCGCAGCGGTCACAGAGATCAGCTTCAGGGA21990.16784760445059488No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21930.1673896300864732No Hit
ATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGT21460.1638021642341867No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC21390.16326786080937808No Hit
GGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAG20920.15968039495709163No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20480.15632191628686598No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA20140.1537267282235098No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19950.15227647607045783No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19920.15204748888839698No Hit
CCATATATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC19660.15006293331053638No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC18870.14403293751626764No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT18750.14311698878802429No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA18660.14243002724184176No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA18510.14128509133153755No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA17900.13662901862963384No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC17660.13479712117314713No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT17570.13411015962696463No Hit
CTACCATACTGCTGACAATAATACACTGCAAAATCTTCAGGCTCCAGTCT17500.133575856202156No Hit
GTATTACTGTGCGAGAGTCGGATGGAGTGGTGGCTACGTCGGGGTCAATG17290.13197294592773012No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC17160.13098066813879983No Hit
CCTCAAGGTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGC17020.1299120612891826No Hit
CTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGT16940.129301428803687No Hit
ATATATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTA16810.1283091510147567No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG16730.12769851852926115No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC16640.12701155698307862No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16610.12678256980101776No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16430.12540864670865273No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16400.12517965952659188No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16370.12495067234453107No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16340.12472168516247022No Hit
GTAATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGA16200.12365307831285297No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAACCT15920.12151586461361848No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15700.11983662527850565No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA15670.11960763809644483No Hit
ACCAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTC15660.11953130903575787No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC15430.1177757406399581No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15400.11754675345789728No Hit
TATATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAA15330.11701245003308866No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC15260.11647814660828003No Hit
CTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCA15230.11624915942621919No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15090.11518055257660194No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG14910.11380662948423691No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14770.11273802263461967No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG14590.11136409954225462No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA14580.11128777048156767No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14490.11060080893538517No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14480.11052447987469823No Hit
GTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCT14380.10976118926782875No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14200.10838726617546372No Hit
GTGTATTATTGTCAGCAGTATGGTAGCTCACCTCAAGGTTTCGGCGGAGG14180.10823460805408983No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC13970.10663169777966396No Hit
CTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCA13950.10647903965829006No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG13870.10586840717279448No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA13780.10518144562661198No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13770.10510511656592503No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGG13700.10457081314111641No Hit
ATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA13580.10365486441287305No Hit
GTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGC13520.10319689004875136No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13510.10312056098806444No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT13460.10273891568462969No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACC13360.10197562507776024No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13130.10022005668196046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)