FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1490968
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCTGTATGGTAGCTCACCCGCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG70190.47076798428940125No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64210.4306598129537321No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT53440.35842486223715064No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC48120.3227433452629433No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC40770.2734465126012094No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39210.26298351138320875No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36670.2459475991436436No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG34570.23186278981171962No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33470.22448503254261662No Hit
GTGTATTACTGTCAGCTGTATGGTAGCTCACCCGCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAA33270.22314362213005243No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT32820.220125448701783No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG32220.21610121746409044No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA31870.2137537492421031No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT31480.21113799893760296No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT31410.21066850529320552No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTAGT31170.2090588127981285No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC30880.2071137676999104No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGACCAGTCAGAGTGTTACC30860.206979626658654No Hit
GTATGGTAGCTCACCCGCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACC29370.1969861190850508No Hit
CTCCTGCAGGACCAGTCAGAGTGTTACCAGCACCTTCGTAGCCTGGTACC29120.19530935606934555No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA28420.1906144196253709No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGACCAGTCAGAGTGTTACCAGC27210.18249888662935757No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG26560.17813930278852397No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC25460.17076154551942094No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACATGGCGGGTGAGCTACCATAC24840.16660317324047197No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA23910.1603656148220485No Hit
CAATAATATACGGCCGTGTCCGCGGCGGTCACAGAGTTCAGCTTCAGGGAGAACTGGTTCTTGGACTGGTCT23710.1590242044094843No Hit
GTCAGAGTGTTACCAGCACCTTCGTAGCCTGGTACCAGCAGAAATCTGGCCTGGCTCCCAGGCTCCTCATCTATG23540.15788400555880475No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23060.15466462056865068No Hit
ATGCTACATCCACTAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTC22740.152518363908548No Hit
CTACCATACAGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC22190.14882948527399648No Hit
ACCATACAGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCC21930.14708565173766305No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGACCAGTCAGA21920.14701858121703484No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG21910.14695151069640663No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC21730.14574424132509886No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21680.1454088887219578No Hit
CATCTATGCTACATCCACTAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTC21400.14353091414436794No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21240.14245778581431662No Hit
TTCGTAGCCTGGTACCAGCAGAAATCTGGCCTGGCTCCCAGGCTCCTCATCTATGCTACATCCACTAGGGCC20750.13917133030353435No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC20730.13903718926227793No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC20340.13642143895777778No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC20080.13467760542144433No Hit
GAGTGTTACCAGCACCTTCGTAGCCTGGTACCAGCAGAAATCTGGCCTGGCTCCCAGGCTCCTCATCTATGCTAC19980.13400690021516223No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19910.13353740657076477No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC19560.13118993834877743No Hit
GTCCTGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC19030.12763520075548235No Hit
GGCCAAAAGTGTACATGGCGGGTGAGCTACCATACAGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGT18970.1272327776317131No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCTGTATGGTAGCTC18860.12649500190480278No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT18710.12548894409537964No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC18690.12535480305412322No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18680.12528773253349504No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18390.12334268743527696No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTAGTGGATGTAGCAT18000.12072693713077678No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC17930.12025744348637933No Hit
CTGCAGGACCAGTCAGAGTGTTACCAGCACCTTCGTAGCCTGGTACCAGCAGAAATCTGGCCTGGCTCCCAGGCT17660.11844653942941767No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT17390.11663563537245601No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAACTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCC17370.1165014943311996No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16870.11314796829978913No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16800.11267847465539166No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16640.11160534632534032No Hit
GTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGACCAGTC16620.1114712052840839No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT16530.11086757059843No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16520.1108005000778018No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC16240.10892252550021193No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16190.10858717289707089No Hit
GTTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCAGTAGACCAGTCCAAGAACCAGTTCTCCCTGAAGCTGA16120.10811767925267342No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15880.1065079867575964No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15570.10442880061812192No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAACTGTGTTGACGCAGTCTC15400.10328860176744235No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15330.1028191081230449No Hit
ATATTATTGTGCGCGCTGTGGCTCGTTTTTACTTGACTATTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGC15260.10234961447864743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)