FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485281
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT90930.6122073870196952No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT87670.590258678324169No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG61020.4108313511046058No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC59620.4014055252844411No Hit
GTATATAGCTTGGTTCCAACAGAAACCAGGAGAGCCTCCTAAGTTACTCATTCACTGGGCGTCTTCCCGAGAACC57050.38410240217171027No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA56260.3787835433160459No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG55980.376898378152013No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT51570.3472070268184943No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG50000.33663663643445246No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT49930.33616534514344426No Hit
CTTTTGGCCAGGGGACCAAGTTACAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG48270.3249890088138204No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC47760.321555315122189No Hit
GATGTATATAGCTTGGTTCCAACAGAAACCAGGAGAGCCTCCTAAGTTACTCATTCACTGGGCGTCTTCCCGAGA46270.31152354335644233No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC46220.31118690672000787No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC43070.2899787986246374No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCGGAGTCTTCTCT42050.28311141124137457No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA41240.27765789773113636No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG40810.2747628226578001No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA39320.2647310508920534No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT38020.25597849834475767No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC37210.25052498483451957No Hit
GTGCAGCCACAGTTCGTTTGACCTGTAACTTGGTCCCCTGGCCAAAAGTGTACGGAAGATTAAAATATTGCAGAC36550.24608138123358475No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC34310.23100005992132128No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC33280.2240653452107716No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG33230.22372870857433713No Hit
CTCCTAAGTTACTCATTCACTGGGCGTCTTCCCGAGAACCCGGGGTCCCT31980.2153127926634758No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCTGC31910.21484150137246757No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG31470.21187909897184437No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT30250.2036651650428437No Hit
GGCCAAAAGTGTACGGAAGATTAAAATATTGCAGACAATAATAAACTGCC30120.20278990978811418No Hit
GTTTATTATTGTCTGCAATATTTTAATCTTCCGTACACTTTTGGCCAGGGGACCAAGTTACAGGTCAAACGA29820.20077008996950743No Hit
GTCCCTGATCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC29820.20077008996950743No Hit
GTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCGGAGTCTTC29730.20016414402392546No Hit
TCCGTACACTTTTGGCCAGGGGACCAAGTTACAGGTCAAACGAACTGTGG29630.19949087075105654No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29510.19868294282361387No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTGTAACTTGGTCCCCTGGCCAAAAGTGTACGGAAGATT29360.19767303291431051No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28850.19423933922267908No Hit
GCTATATACATCTTATTATTGAAGCCGTAGAGAAGACTCCGGCTGGACTTGCAGCTGATGGTGGCCCTCTCGCCC28660.19296012000422816No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCTGCAATATTTTAATCTT28200.18986306294903119No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC27910.18791057045771137No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT27220.18326498487491594No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC27000.18178378367460435No Hit
CTTCTCTACGGCTTCAATAATAAGATGTATATAGCTTGGTTCCAACAGAAACCAGGAGAGCCTCCTAAGTTACTC26720.1798986185105714No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA26540.17868672661940738No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT26030.17525303292777594No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCA25930.17457975965490705No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT25910.17444510500033328No Hit
TTATTATTGTCTGCAATATTTTAATCTTCCGTACACTTTTGGCCAGGGGA25820.17383915905475125No Hit
ATATACATCTTATTATTGAAGCCGTAGAGAAGACTCCGGCTGGACTTGCAGCTGATGGTGGCCCTCTCGCCCAG25040.1685876275263738No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT24300.1636054053071439No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23930.16111429419752896No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC23550.1585558557606271No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG23480.15808456446961888No Hit
GGCTGAAGATGTGGCAGTTTATTATTGTCTGCAATATTTTAATCTTCCGTACACTTTTGGCCAGGGGACCAAGTT23440.15781525516047132No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT23150.1558627626691515No Hit
ACTTAGGAGGCTCTCCTGGTTTCTGTTGGAACCAAGCTATATACATCTTA23070.15532414405085637No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG22930.15438156146883988No Hit
AACCAGGAGAGCCTCCTAAGTTACTCATTCACTGGGCGTCTTCCCGAGAA22670.15263105095938076No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC22530.15168846837736427No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC22470.15128450441364294No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT22170.14926468459503622No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG22150.14913002994046246No Hit
CTCATTCACTGGGCGTCTTCCCGAGAACCCGGGGTCCCTGATCGATTCAGTGGCAGCGGGTCTGGGACAGATTTC22090.1487260659767411No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22030.14832210201301974No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22020.14825477468573287No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCTGCAAT22010.148187447358446No Hit
GGATCTCTGGTGCCCACGGCGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG21550.145090390303249No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC21540.14502306297596212No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC21420.14421513504851946No Hit
GTTACAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGG21040.1416566966116176No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT20990.14132005997518315No Hit
GTGAATGAGTAACTTAGGAGGCTCTCCTGGTTTCTGTTGGAACCAAGCTATATACATCTTATTATTGAAGCCGT20640.13896360352014198No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA20640.13896360352014198No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTC20270.13647249241052703No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC19840.13357741733719075No Hit
ATTTTAATCTTCCGTACACTTTTGGCCAGGGGACCAAGTTACAGGTCAAA19710.13270216208246116No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT19480.13115363355486268No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19310.13000906899098555No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG19300.12994174166369865No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCGGAGTCTTCTCTACGGCTTCAATA19270.12973975968183799No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT19240.1295377776999773No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA19180.12913381373625596No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19100.12859519511796083No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTC19000.12792192184509193No Hit
ATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCC18880.12711399391764924No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT18820.1267100299539279No Hit
CCACTGAATCGATCAGGGACCCCGGGTTCTCGGGAAGACGCCCAGTGAAT18780.12644072064478035No Hit
ATATAGCTTGGTTCCAACAGAAACCAGGAGAGCCTCCTAAGTTACTCATT18520.1246902101353212No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG17830.12004462455252575No Hit
GATCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGC17620.11863075067950105No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17470.1176208407701977No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17120.11526438431515654No Hit
GTAGAGAAGACTCCGGCTGGACTTGCAGCTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGG16990.11438912906042696No Hit
AGTTACAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG16890.11371585578755805No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCCACGGCGACATCGTGATG16880.11364852846027117No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG16860.11351387380569737No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT16830.1133118918238367No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGC16810.11317723716926292No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGG16670.11223465458724646No Hit
ATCAGGGACCCCGGGTTCTCGGGAAGACGCCCAGTGAATGAGTAACTTAGGAGGCTCTCCTGGTTTCTGTTGGA16630.11196534527809889No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC16560.11149405398709067No Hit
CAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCC16490.11102276269608242No Hit
GTGTACGGAAGATTAAAATATTGCAGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAG16490.11102276269608242No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT16420.11055147140507418No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA16350.11008018011406596No Hit
CTGGTGCCCACGGCGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCT16120.10853165158646748No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16090.10832966960460681No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC15850.10671381374972144No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC15780.1062425224587132No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT15760.10610786780413942No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT15690.10563657651313119No Hit
GTTCCAACAGAAACCAGGAGAGCCTCCTAAGTTACTCATTCACTGGGCGT15220.10247219213064733No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG15040.10126030023948329No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA15020.10112564558490954No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC14980.10085633627576196No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTC14860.10004840834831928No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)