FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485281
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT87130.5866230026506769No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT77210.5198342939820815No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA59640.40154017993901486No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC56720.3818806003712429No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG53090.3574407805661016No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT50860.342426786581125No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT47970.3229691889952137No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG47320.3185929127215658No Hit
CTTTTGGCCAGGGGACCAAGTTACAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG45450.3060027025189173No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC44420.2990679878083676No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC43530.2930758556798343No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC42550.2864777776057191No Hit
GTGCAGCCACAGTTCGTTTGACCTGTAACTTGGTCCCCTGGCCAAAAGTGTACGGAAGATTAAAATATTGCAGAC41660.2804856454771858No Hit
GTATATAGCTTGGTTCCAACAGAAACCAGGAGAGCCTCCTAAGTTACTCATTCACTGGGCGTCTTCCCGAGAACC41590.2800143541861776No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG40580.27321429413020165No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG40120.2701172370750046No Hit
GGCCAAAAGTGTACGGAAGATTAAAATATTGCAGACAATAATAAACTGCC39540.266212252092365No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA38610.25995081065468423No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC38040.25611315299933146No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT37310.25119825810738844No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT36590.24635069054273231No Hit
ATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCC33980.22877825812085392No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG33540.2258158557202307No Hit
GATGTATATAGCTTGGTTCCAACAGAAACCAGGAGAGCCTCCTAAGTTACTCATTCACTGGGCGTCTTCCCGAGA33070.22265147133774688No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCGGAGTCTTCTCT33070.22265147133774688No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT32720.2202950148827057No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA31710.21349495482672975No Hit
GTCCCAGACCCGCTGCCACTGAATCGATCAGGGACCCCGGGTTCTCGGGAAGACGCCCAGTGAATGAGTAACTT31310.21080186173525411No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTGTAACTTGGTCCCCTGGCCAAAAGTGTACGGAAGATT30830.20757015002548337No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC30350.20433843831571263No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC29920.20144336324237636No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29600.19928888876919584No Hit
GTTTATTATTGTCTGCAATATTTTAATCTTCCGTACACTTTTGGCCAGGGGACCAAGTTACAGGTCAAACGA28890.19450864853182664No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC28780.19376804793167085No Hit
CAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCC28670.19302744733151503No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCTGC28380.19107495484019524No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC28160.18959375363988362No Hit
GCTATATACATCTTATTATTGAAGCCGTAGAGAAGACTCCGGCTGGACTTGCAGCTGATGGTGGCCCTCTCGCCC27670.186294714602826No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC27620.18595807796639155No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG27130.1826590389293339No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCTGCAATATTTTAATCTT26800.18043723712886653No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT25820.17383915905475125No Hit
GTCCCTGATCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC25650.17269459449087413No Hit
TCCGTACACTTTTGGCCAGGGGACCAAGTTACAGGTCAAACGAACTGTGG25260.17006882872668538No Hit
GGCTGAAGATGTGGCAGTTTATTATTGTCTGCAATATTTTAATCTTCCGTACACTTTTGGCCAGGGGACCAAGTT25210.16973219209025092No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGATCAGGGACC24960.16804900890807867No Hit
CTCCTAAGTTACTCATTCACTGGGCGTCTTCCCGAGAACCCGGGGTCCCT24760.16670246236234085No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC24440.16454798788916036No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC24330.16380738728900457No Hit
TTATTATTGTCTGCAATATTTTAATCTTCCGTACACTTTTGGCCAGGGGA23730.15976774765179114No Hit
ACTTAGGAGGCTCTCCTGGTTTCTGTTGGAACCAAGCTATATACATCTTA23650.159229129033496No Hit
ATATACATCTTATTATTGAAGCCGTAGAGAAGACTCCGGCTGGACTTGCAGCTGATGGTGGCCCTCTCGCCCAG23470.15801723714233198No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG23010.154920180087135No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22840.15377561552325789No Hit
GTGTACGGAAGATTAAAATATTGCAGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAG22640.15242906897752007No Hit
GTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCGGAGTCTTC22520.1516211410500774No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22500.1514864863955036No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG22160.14919735726774933No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC22130.14899537528588866No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGATCAGGGACCCCG21890.14737951943100328No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21870.1472448647764295No Hit
GTGAATGAGTAACTTAGGAGGCTCTCCTGGTTTCTGTTGGAACCAAGCTATATACATCTTATTATTGAAGCCGT21870.1472448647764295No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC21570.1452250449578228No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT21530.14495573564867525No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA21480.1446190990122408No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21450.14441711703038013No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21420.14421513504851946No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCA20840.1403101500658798No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCTGCAAT20280.13653981973781393No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC20220.13613585577409257No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20210.1360685284468057No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT20180.13586654646494503No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT19690.13256750742788737No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19540.13155759751858404No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC19530.13149027019129714No Hit
CTTCTCTACGGCTTCAATAATAAGATGTATATAGCTTGGTTCCAACAGAAACCAGGAGAGCCTCCTAAGTTACTC19190.12920114106354286No Hit
GTTACAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGG19130.1287971770998215No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA19020.12805657649966573No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGATCAGGGACCCCGGGTTCTCGGGA18730.12610408400834588No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT18550.12489219211718186No Hit
ATTTTAATCTTCCGTACACTTTTGGCCAGGGGACCAAGTTACAGGTCAAA18370.12368030022601785No Hit
CCACTGAATCGATCAGGGACCCCGGGTTCTCGGGAAGACGCCCAGTGAAT17960.12091987980725533No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG17650.11883273266136173No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG17470.1176208407701977No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17100.11512972966058274No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17090.11506240233329584No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA17080.11499507500600896No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17070.11492774767872208No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17000.11445645638771385No Hit
GCCACAGTTCGTTTGACCTGTAACTTGGTCCCCTGGCCAAAAGTGTACGG16980.11432180173314005No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16900.11378318311484492No Hit
ATCAGGGACCCCGGGTTCTCGGGAAGACGCCCAGTGAATGAGTAACTTAGGAGGCTCTCCTGGTTTCTGTTGGA16680.11230198191453335No Hit
CTCATTCACTGGGCGTCTTCCCGAGAACCCGGGGTCCCTGATCGATTCAGTGGCAGCGGGTCTGGGACAGATTTC16640.1120326726053858No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16640.1120326726053858No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC16610.1118306906235251No Hit
ACCTGTAACTTGGTCCCCTGGCCAAAAGTGTACGGAAGATTAAAATATTG16510.1111574173506562No Hit
AACCAGGAGAGCCTCCTAAGTTACTCATTCACTGGGCGTCTTCCCGAGAA16440.11068612605964798No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16350.11008018011406596No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCT16340.11001285278677907No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG16240.10933957951391016No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC16230.10927225218662327No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16150.10873363356832814No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGG16130.10859897891375436No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15880.10691579573158211No Hit
AGTTACAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG15770.10617519513142631No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT15370.1034821020399507No Hit
CTCCTGGTTTCTGTTGGAACCAAGCTATATACATCTTATTATTGAAGCCGTAGAGAAGACTCCGGCTGGACTTGC15310.10307813807622934No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT15270.10280882876708179No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)