FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210227
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59640.49280011105354615No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT55130.45553437495610327No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTTCCA45180.3733183939872437No Hit
GAGTTTAGTCTCACCATCACCAGCCTGCAGCTTGAAGACTCTGCTAGTTATTACTGCCAACAATATGAGGGTTTT41960.34671181522144195No Hit
GGGTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACCACCTACCA40390.3337390423449485No Hit
CCTTCCACCCTGTCTGCGTCTCTTGGAGACAAAGTCACCATCACTTGCCG39520.3265503083305859No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38310.3165521840117598No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37610.31076814514962897No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG35650.2945728363356626No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG34240.2829221294847991No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA32540.2688751779624814No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32390.2676357410634534No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTCGGAAAACCCTCATATTGTTGGCAGT30870.2550761138199693No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC29560.24425169823512446No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTTCCAAATGTGACATCC29240.2416075661838647No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA29150.24086390404444788No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT28590.23623667295474318No Hit
GACTAAACTCTGTCCCAGTTCCGGTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAATGAGACGCCCGAG28210.23309676614387217No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTTCCAAATGTGACAT27970.23111366710542736No Hit
GTAGTAGACGGCCGTGTCGTCAGATGTGAGGTTGCTCAGTTCCAGGTAGGCAGTGGTGATGGCTGTGTCCCTCGT26960.22276812531863854No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26750.2210329136599993No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG25700.2123568553668031No Hit
CCCCTACCCTCCTCATCTCTCGGGCGTCTCATTTAGAAAGTGGGGTCCCA25450.21029112720175636No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA23350.1929390106153639No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22940.18955121642468725No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22930.18946858729808538No Hit
GTCTCCTTCCACCCTGTCTGCGTCTCTTGGAGACAAAGTCACCATCACTT22880.18905544166507607No Hit
AGTTATTACTGCCAACAATATGAGGGTTTTCCGACGTTCGGCCAAGGGAC22250.18384980668915832No Hit
GGTATCAGCAGAGACCAGGGAAAGCCCCTACCCTCCTCATCTCTCGGGCGTCTCATTTAGAAAGTGGGGTCCCAT21940.1812883037645004No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21700.17930520472605554No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTCGGAAAACCCTC21490.17756999306741628No Hit
GAACTGGGACAGAGTTTAGTCTCACCATCACCAGCCTGCAGCTTGAAGACTCTGCTAGTTATTACTGCCAACAAT21230.1754216357757677No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT20700.17104229206586863No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20270.16748923962198828No Hit
GTCTGCGTCTCTTGGAGACAAAGTCACCATCACTTGCCGGGCCAGTCAGA19840.16393618717810793No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19770.16335778329189482No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC19640.16228360464607053No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTTCCAA19370.16005261822782008No Hit
CACCATCACCAGCCTGCAGCTTGAAGACTCTGCTAGTTATTACTGCCAACAATATGAGGGTTTTCCGACGTTCGG19160.15831740656918084No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC18860.15583853277112475No Hit
GACCAGGGAAAGCCCCTACCCTCCTCATCTCTCGGGCGTCTCATTTAGAA18840.155673274517921No Hit
TCTCTCGGGCGTCTCATTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCACCGGAACTGGGACAGAGTTTAGT18800.15534275801151354No Hit
GTCTTCAAGCTGCAGGCTGGTGATGGTGAGACTAAACTCTGTCCCAGTTCCGGTGCCGCTGAACCTTGATGGGAC18560.1533596589730687No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18490.1527812550868556No Hit
GTAATAACTAGCAGAGTCTTCAAGCTGCAGGCTGGTGATGGTGAGACTAAACTCTGTCCCAGTTCCGGTGCCG18380.15187233469423506No Hit
CATTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCACCGGAACTGGGACAGAGTTTAGTCTCACCATCACCAGC18290.1511286725548182No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC18270.15096341430161447No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTTCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC18270.15096341430161447No Hit
CTGCTGATACCAGGCCAACCACCTACCAACATTCTGACTGGCCCGGCAAGTGATGGTGACTTTGTCTCCAAG18250.15079815604841076No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT17900.14790613661734534No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC17870.14765824923753973No Hit
GCTGATACCAGGCCAACCACCTACCAACATTCTGACTGGCCCGGCAAGTGATGGTGACTTTGTCTCCAAGAGACG17560.1450967463128818No Hit
TCCCTGGTCTCTGCTGATACCAGGCCAACCACCTACCAACATTCTGACTGGCCCGGCAAGTGATGGTGACTTTGT17160.14179158124880703No Hit
TCACCAGCCTGCAGCTTGAAGACTCTGCTAGTTATTACTGCCAACAATAT17030.14071740260298277No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16960.14013899871676966No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16940.13997374046356592No Hit
GTCAGAATGTTGGTAGGTGGTTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTACCCTCCTCATCTCTCGGG16670.13774275404531547No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16550.13675120452609305No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16550.13675120452609305No Hit
ATATGAGGGTTTTCCGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT16510.13642068801968557No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC15950.1317934569299809No Hit
GCTTGAAGACTCTGCTAGTTATTACTGCCAACAATATGAGGGTTTTCCGACGTTCGGCCAAGGGACCAGGGTGGA15910.13146294042357343No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15890.1312976821703697No Hit
GTATCAGCAGAGACCAGGGAAAGCCCCTACCCTCCTCATCTCTCGGGCGTCTCATTTAGAAAGTGGGGTCCCATC15770.13030613265114727No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC15710.12981035789153605No Hit
TTCTAAATGAGACGCCCGAGAGATGAGGAGGGTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACCACCT15700.12972772876493419No Hit
GTCCCAGTTCCGGTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAATGAGACGCCCGAGAGATGAGGAGGGT15650.12931458313192484No Hit
CCTCATCTCTCGGGCGTCTCATTTAGAAAGTGGGGTCCCATCAAGGTTCA15520.12824040448610052No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15340.1267530802072669No Hit
CAATATGAGGGTTTTCCGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCAT15320.12658782195406315No Hit
CCCGAGAGATGAGGAGGGTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACCACCTACCAACATTCTG15130.12501786854862765No Hit
ACCTTGATGGGACCCCACTTTCTAAATGAGACGCCCGAGAGATGAGGAGGGTAGGGGCTTTCCCTGGTCTCTG15120.12493523942202578No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTTCCAAATGTGACATCCAGATG14980.1237784316495996No Hit
GTCTACTACTGTGCGAGAGAGGGGGGCCCGGGATATTGTAGCAGTGCCAACTGCTATGGATTCGACCCCTGGGGT14910.12320002776338654No Hit
CCCTCATATTGTTGGCAGTAATAACTAGCAGAGTCTTCAAGCTGCAGGCTGGTGATGGTGAGACTAAACTCTGTC14840.12262162387717346No Hit
ACCCAGTCTCCTTCCACCCTGTCTGCGTCTCTTGGAGACAAAGTCACCAT14420.11915120055989495No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC14270.11791176366086693No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14130.11675495588844076No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13960.11535026073620899No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13330.11014462576029126No Hit
GTTGGTAGGTGGTTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAC13330.11014462576029126No Hit
CTGCTAGTTATTACTGCCAACAATATGAGGGTTTTCCGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGA13080.10807889759524453No Hit
AACTAGCAGAGTCTTCAAGCTGCAGGCTGGTGATGGTGAGACTAAACTCTGTCCCAGTTCCGGTGCCGCTGAAC13040.10774838108883705No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT12990.10733523545582771No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12980.10725260632922584No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12970.10716997720262397No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12920.10675683156961462No Hit
CTTCCACCCTGTCTGCGTCTCTTGGAGACAAAGTCACCATCACTTGCCGG12890.10650894418980901No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12860.1062610568100034No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12770.1055173946705866No Hit
GCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGC12730.10518687816417911No Hit
GCTCTGGCTCCCAGGTTCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCGTCTCTTGG12550.10369955388534546No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12440.10279063349272492No Hit
GCCCCTACCCTCCTCATCTCTCGGGCGTCTCATTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCACCGGA12430.10270800436612305No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12420.10262537523952119No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTC12320.10179908397350249No Hit
GGGAAAGCCCCTACCCTCCTCATCTCTCGGGCGTCTCATTTAGAAAGTGG12220.10097279270748381No Hit
CCTACCAACATTCTGACTGGCCCGGCAAGTGATGGTGACTTTGTCTCCAAGAGACGCAGACAGGGTGGAAGG12200.10080753445428008No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12190.10072490532767821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)