FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210227
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57820.477761610012006No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT47090.3891005571682007No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTTCCA41520.34307613365095974No Hit
GAGTTTAGTCTCACCATCACCAGCCTGCAGCTTGAAGACTCTGCTAGTTATTACTGCCAACAATATGAGGGTTTT41060.3392751938272737No Hit
GGGTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACCACCTACCA39720.32820289086262333No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA35910.29672119362731125No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35080.28986297611935613No Hit
CCTTCCACCCTGTCTGCGTCTCTTGGAGACAAAGTCACCATCACTTGCCG34890.2882930227139206No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34240.2829221294847991No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33100.2735024090521861No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG30860.25499348469336747No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTCGGAAAACCCTCATATTGTTGGCAGT29610.24466484386813384No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28650.2367324477143544No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC28650.2367324477143544No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT28030.23160944186503854No Hit
GACTAAACTCTGTCCCAGTTCCGGTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAATGAGACGCCCGAG27230.22499911173688905No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA26480.21880192724174885No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTTCCAAATGTGACAT26140.21599253693728532No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTTCCAAATGTGACATCC25560.21120004759437694No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG23740.1961615465528368No Hit
GTAGTAGACGGCCGTGTCGTCAGATGTGAGGTTGCTCAGTTCCAGGTAGGCAGTGGTGATGGCTGTGTCCCTCGT23690.19574840091982743No Hit
CCCCTACCCTCCTCATCTCTCGGGCGTCTCATTTAGAAAGTGGGGTCCCA23510.1942610766409938No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23320.1926911232355583No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC23120.19103854070352091No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22760.18806389214585362No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG22240.18376717756255645No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22170.18318877367634337No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21980.18161882027090787No Hit
GTCTCCTTCCACCCTGTCTGCGTCTCTTGGAGACAAAGTCACCATCACTT21450.1772394765610088No Hit
AGTTATTACTGCCAACAATATGAGGGTTTTCCGACGTTCGGCCAAGGGAC21260.1756695231555733No Hit
GGTATCAGCAGAGACCAGGGAAAGCCCCTACCCTCCTCATCTCTCGGGCGTCTCATTTAGAAAGTGGGGTCCCAT21040.1738516823703322No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20760.17153806682547987No Hit
GAACTGGGACAGAGTTTAGTCTCACCATCACCAGCCTGCAGCTTGAAGACTCTGCTAGTTATTACTGCCAACAAT20370.16831553088800694No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT19700.16277937940568174No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTCGGAAAACCCTC19130.15806951918937523No Hit
CTGCTGATACCAGGCCAACCACCTACCAACATTCTGACTGGCCCGGCAAGTGATGGTGACTTTGTCTCCAAG19060.15749111530316212No Hit
CACCATCACCAGCCTGCAGCTTGAAGACTCTGCTAGTTATTACTGCCAACAATATGAGGGTTTTCCGACGTTCGG18880.1560037910243285No Hit
GTCTTCAAGCTGCAGGCTGGTGATGGTGAGACTAAACTCTGTCCCAGTTCCGGTGCCGCTGAACCTTGATGGGAC18780.1551774997583098No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA18780.1551774997583098No Hit
TCTCTCGGGCGTCTCATTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCACCGGAACTGGGACAGAGTTTAGT18330.1514591890612257No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC18290.1511286725548182No Hit
GTCTGCGTCTCTTGGAGACAAAGTCACCATCACTTGCCGGGCCAGTCAGA18270.15096341430161447No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC18250.15079815604841076No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC18070.1493108317695771No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17930.14815402399715094No Hit
GTAATAACTAGCAGAGTCTTCAAGCTGCAGGCTGGTGATGGTGAGACTAAACTCTGTCCCAGTTCCGGTGCCG17460.14427045504686312No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTTCCAA17430.1440225676670575No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC17350.14336153465424253No Hit
GACCAGGGAAAGCCCCTACCCTCCTCATCTCTCGGGCGTCTCATTTAGAA17080.14113054823599208No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16870.13939533657735284No Hit
CATTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCACCGGAACTGGGACAGAGTTTAGTCTCACCATCACCAGC16620.13732960841230613No Hit
TCCCTGGTCTCTGCTGATACCAGGCCAACCACCTACCAACATTCTGACTGGCCCGGCAAGTGATGGTGACTTTGT16450.13592491326007433No Hit
GTCCCAGTTCCGGTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAATGAGACGCCCGAGAGATGAGGAGGGT16440.13584228413347246No Hit
ATATGAGGGTTTTCCGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT16160.13352866858862014No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTTCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC16160.13352866858862014No Hit
GCTGATACCAGGCCAACCACCTACCAACATTCTGACTGGCCCGGCAAGTGATGGTGACTTTGTCTCCAAGAGACG16010.1322892316895921No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15970.13195871518318464No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC15870.13113242391716595No Hit
GCTTGAAGACTCTGCTAGTTATTACTGCCAACAATATGAGGGTTTTCCGACGTTCGGCCAAGGGACCAGGGTGGA15680.12956247051173045No Hit
GTATCAGCAGAGACCAGGGAAAGCCCCTACCCTCCTCATCTCTCGGGCGTCTCATTTAGAAAGTGGGGTCCCATC15580.12873617924571176No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT15570.1286535501191099No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC15540.1284056627393043No Hit
CCTCATCTCTCGGGCGTCTCATTTAGAAAGTGGGGTCCCATCAAGGTTCA15520.12824040448610052No Hit
GTCAGAATGTTGGTAGGTGGTTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTACCCTCCTCATCTCTCGGG15210.12567890156144262No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15130.12501786854862765No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15120.12493523942202578No Hit
GGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG15040.12427420640921084No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14770.12204321999096036No Hit
TCACCAGCCTGCAGCTTGAAGACTCTGCTAGTTATTACTGCCAACAATAT14390.11890331318008934No Hit
TTCTAAATGAGACGCCCGAGAGATGAGGAGGGTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACCACCT14350.11857279667368188No Hit
GTCTACTACTGTGCGAGAGAGGGGGGCCCGGGATATTGTAGCAGTGCCAACTGCTATGGATTCGACCCCTGGGGT14350.11857279667368188No Hit
CCCTCATATTGTTGGCAGTAATAACTAGCAGAGTCTTCAAGCTGCAGGCTGGTGATGGTGAGACTAAACTCTGTC14190.11725073064805197No Hit
CCCGAGAGATGAGGAGGGTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACCACCTACCAACATTCTG14120.11667232676183889No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14050.11609392287562582No Hit
ACCCAGTCTCCTTCCACCCTGTCTGCGTCTCTTGGAGACAAAGTCACCAT13750.11361504907756975No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTTCCAAATGTGACATCCAGATG13750.11361504907756975No Hit
CTGCTGCTCTGGCTCCCAGGTTCCAAATGTGACATCCAGATGACCCAGTC13740.11353241995096788No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC13660.11287138693815292No Hit
ACCTTGATGGGACCCCACTTTCTAAATGAGACGCCCGAGAGATGAGGAGGGTAGGGGCTTTCCCTGGTCTCTG13620.11254087043174545No Hit
CTGCTAGTTATTACTGCCAACAATATGAGGGTTTTCCGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGA13560.11204509567213423No Hit
CAATATGAGGGTTTTCCGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCAT13490.11146669178592114No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG13370.11047514226669873No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13360.11039251314009686No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGAACCTGGGAGCCAGAGCAGCAGGAGCCCCAGG13230.10931833449427256No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13120.10840941410165202No Hit
GTTGGTAGGTGGTTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAC12920.10675683156961462No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12910.10667420244301275No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12480.1031211499991324No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12330.10188171310010435No Hit
GCTCTGGCTCCCAGGTTCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCGTCTCTTGG12220.10097279270748381No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12220.10097279270748381No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)