FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202953
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGGCTGTGTTTCCTGACCTGGATGC34040.28297032386136445No Hit
CACCTGCACTGGGAACAGCAACAATGTTGGCAACCAAGGAGCAGCTTGGCTGCAGCAGCACCAGGGCCACCCTCC31710.2636013210823698No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30160.2507163621521373No Hit
GACTATTACTGCTCAGCATGGGACAGCAGCCTCAGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCT27540.22893662512167975No Hit
GTCCCATGCTGAGCAGTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGGCTGTGTTTC27360.22744030731042691No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA26990.2243645429206295No Hit
GCATGGGACAGCAGCCTCAGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG26510.220374362090622No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACTGAGGCTGCTGTCCCATG25920.21546976482040445No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25080.20848694836789133No Hit
TTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGCTCAGCATGG24570.2042473812360084No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACTGAGGCTGCTGTCCCATGCTGAGCAGTAAT24320.20216916205371283No Hit
GTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC22020.1830495455765936No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21220.1763992441932478No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20640.17157777569032207No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20620.1714115181557384No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20540.17074648801740383No Hit
CCCAAGCACTGAGGCTGCTGTCCCATGCTGAGCAGTAATAGTCAGCCTCG20380.16941642774073468No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC20260.16841888253323278No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC20120.1672550797911473No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20080.16692256472198No Hit
GTCCAGTAATGGTCAGGGAGGCTGTGTTTCCTGACCTGGATGCAGATAAT20030.16650692088552088No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT19930.16567563321260265No Hit
CTCCTATCCTACAGGAATAACAACCGGCCCTCAGGGATCTCAGAGAGATTATCTGCATCCAGGTCAGGAAACAC19650.16334802772843163No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC18290.15204251537674374No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17260.14348025234568598No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17250.14339712357839415No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16960.1409863893269313No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTGTGCTCGTGGATTCGTCCGCGGT16950.14090326055963948No Hit
GTCCAAGGGCTTGAGACAGACCGCCACACTCACCTGCACTGGGAACAGCAACAATGTTGGCAACCAAGGAGC16100.13383731533983456No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG16070.1335879290379591No Hit
GGCTTGAGACAGACCGCCACACTCACCTGCACTGGGAACAGCAACAATGTTGGCAACCAAGGAGCAGCTTGGCTG15470.12860020300044972No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14890.12377873449752402No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC14810.12311370435918942No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14710.1222824166862712No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14610.12145112901335298No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14540.12086922764231023No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14210.11812597832168006No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14090.11712843311417818No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG13980.11621401667396813No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT13890.11546585776834174No Hit
CCCAAACTCCTATCCTACAGGAATAACAACCGGCCCTCAGGGATCTCAGAGAGATTATCTGCATCCAGGTCAGGA12960.10773488241020222No Hit
GTGGGAATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTCCTCAC12820.10657107966811671No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG12790.10632169336624124No Hit
GGGCTGACTCAGCCACCCTCGGTGTCCAAGGGCTTGAGACAGACCGCCACACTCACCTGCACTGGGAACAGCAAC12760.10607230706436578No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12370.1028302851399847No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC12100.10058580842310548No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12090.10050267965581365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)