FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1414586
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATGCAGTGAGTGCTTGGCGTTGTCTCTGG72230.5106087576153022No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43840.30991399603841685No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT41940.2964825044217884No Hit
GTGTATTACTGTGCGAGAGAGGGGACTGGTGGTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTC41750.2951393552601256No Hit
CTCCTGCAGGGCCAGTCAGAGCGTTTACAGCGGCCACATAGCCTGGTACC32340.2286181257272446No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC32220.22776982099356277No Hit
GTCTGGGACAGACTTCATTCTCACCATCAGCAGACTGGAGCCTGAGGATT31170.22034715457384701No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27790.1964532379084764No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATG26270.18570804461517362No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT25790.18231482568044644No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG25520.18040614002966238No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25450.17991129560168134No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA24510.17326624185450726No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24290.17171101650942394No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG23960.169378178491799No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG23780.1681057213912763No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGTGCCGCCCTCTCCTGCAGGGCCAGTCAGAGCGTTTAC23340.16499527070110973No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGATCTGGG23180.16386419772286734No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22840.16146066764410222No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT22350.1579967566482349No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTATTTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGAGG22030.1557346106917501No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT21450.1516344711456214No Hit
GCCTTAGACCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC21330.15078616641193962No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT20640.14590841419326928No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20520.14506010945958747No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG20480.14477734121502686No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG20390.1441411126647655No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGTGCCGCCCTCTCCTGC20370.14399972854248522No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20240.14308073174766328No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC19850.14032374136319742No Hit
GCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGCCGCATCCAACAGGGCCACTGGCATC19720.13940474456837548No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC19400.13714259861189068No Hit
GTATTTCTGTCAGCTGTATGGTGACTTCAAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGG18920.1337493796771635No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18800.1329010749434817No Hit
CATCTATGCCGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTG18610.13155792578181885No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18470.13056823692585676No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC18320.1295078560087545No Hit
GTATTACTGTGCGAGAGAGGGGACTGGTGGTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGC17940.12682155768542883No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA17870.12632671325744776No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17800.1258318688294667No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTCTTGAAGTCACCATACAGCTGACAGA17550.12406456730096296No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC16900.11946958332685323No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC16590.11727812943150859No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16480.11650051675896693No Hit
GTGTATTTCTGTCAGCTGTATGGTGACTTCAAGACGTTCGGCCAAGGGAC16200.11452113904704274No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC16190.11445044698590259No Hit
GTCTTGAAGTCACCATACAGCTGACAGAAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAATG16160.11423837080248214No Hit
CCATACAGCTGACAGAAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAATGAAGTCTGTCCC16130.11402629461906169No Hit
ACATAAAGCAAGACGGAAGTGAGAGTTCCTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG15980.11296591370195944No Hit
GTCTTTGTCTCCAGGGGAAAGTGCCGCCCTCTCCTGCAGGGCCAGTCAGA15950.11275383751853899No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15870.1121883010294178No Hit
CTTCAAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGG15840.11197622484599734No Hit
GTCTCAGAGAGGAGCCTTAGACCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTC15750.111339996295736No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC15650.11063307568433449No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15450.1092192344615315No Hit
CTCCAGAGACAACGCCAAGCACTCACTGCATCTGCAAATGAATAGCCTGAGAGCCGAGGACACGGCTGTGTATT15390.1087950820946906No Hit
GTGCAGGTGAAGGTCTCCCCATGGTTCCATGGCTGGGCACAGCCAGGCAGGACACTGGACACGCTGTAGCAGCC15240.10773470117758836No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15210.1075226249941679No Hit
GCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGATCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCC15070.1065329361382058No Hit
GACTTCATTCTCACCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTGTATTTCTGTCAGCTGTATGGTGACTTC14820.10476563460970205No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTCTTGAAGTCACC14750.104270790181721No Hit
GGCATGCGACGACCACGTTCCCATCTTGGGGGGTGCTGTCGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGG14710.10398802193716042No Hit
GTATGGTGACTTCAAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTT14600.10321040926461876No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGT14580.10306902514233846No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC14540.10278625689777786No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14380.10165518391953549No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14360.10151379979725518No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGTATTTATTGG14230.10059480300243322No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAACCACCAGTCC14190.10031203475787262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)