FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1414586
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATGCAGTGAGTGCTTGGCGTTGTCTCTGG64720.45751901969904973No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42070.29740150121661035No Hit
GTGTATTACTGTGCGAGAGAGGGGACTGGTGGTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTC39880.28191993982691754No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT36250.2562587216330432No Hit
GTCTGGGACAGACTTCATTCTCACCATCAGCAGACTGGAGCCTGAGGATT31180.2204178466349872No Hit
CTCCTGCAGGGCCAGTCAGAGCGTTTACAGCGGCCACATAGCCTGGTACC29740.21023818983080564No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC29330.20733981532405948No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26840.18973749210016216No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26260.18563735255403346No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA24350.17213516887626484No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24160.170792019714602No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT23510.16619703574049227No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG22930.16209689619436357No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATG22890.161814127949803No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC22790.16110720733840148No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22520.15919852168761744No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTATTTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGAGG22160.15665360748657206No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT21510.1520586235124623No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGATCTGGG21230.1500792458005381No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21180.14972578549483734No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT20470.14470664915388673No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGTGCCGCCCTCTCCTGCAGGGCCAGTCAGAGCGTTTAC20080.14194965876942087No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGTGCCGCCCTCTCCTGC19580.1384150557124134No Hit
GCCTTAGACCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC19290.13636498593934904No Hit
GTATTTCTGTCAGCTGTATGGTGACTTCAAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGG18600.1314872337206787No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18560.1312044654761181No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG18460.1304975448647166No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18370.12986131631445524No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18080.12781124654139092No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG17780.1256904847071864No Hit
GCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGCCGCATCCAACAGGGCCACTGGCATC17570.12420595142324327No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG17560.12413525936210312No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTCTTGAAGTCACCATACAGCTGACAGA17560.12413525936210312No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG17470.12349903081184177No Hit
CATCTATGCCGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTG17400.12300418638386072No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC17320.12243864989473952No Hit
GTATTACTGTGCGAGAGAGGGGACTGGTGGTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGC16700.11805574210405022No Hit
GTGTATTTCTGTCAGCTGTATGGTGACTTCAAGACGTTCGGCCAAGGGAC16450.11628844057554649No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16450.11628844057554649No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16420.11607636439212603No Hit
GTCTTGAAGTCACCATACAGCTGACAGAAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAATG16270.11501598347502379No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC16040.11339006606880034No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC15900.11240037721283824No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15890.1123296851516981No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC15820.11183484072371705No Hit
GTCTTTGTCTCCAGGGGAAAGTGCCGCCCTCTCCTGCAGGGCCAGTCAGA15740.11126930423459584No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15520.10971407888951255No Hit
GTCTCAGAGAGGAGCCTTAGACCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTC15380.10872439003355046No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC15320.10830023766670956No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC15090.10667432026048611No Hit
ACATAAAGCAAGACGGAAGTGAGAGTTCCTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG14870.10511909491540282No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA14800.10462425048742177No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14770.1044121743040013No Hit
CTCCAGAGACAACGCCAAGCACTCACTGCATCTGCAAATGAATAGCCTGAGAGCCGAGGACACGGCTGTGTATT14610.1032811013257589No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14580.10306902514233846No Hit
CCATACAGCTGACAGAAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAATGAAGTCTGTCCC14560.10292764102005816No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCCAG14300.10108964743041426No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTCTTGAAGTCACC14270.10087757124699383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)