FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840581
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24120.28694438727499194No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCACCCAGGCTGTTGTCATAGG22950.27302544311613036No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21490.2556565042512262No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCACCCAGGCTGTTGTCATAGGACTGGCAGTGAT21130.25137375220234576No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19890.23662205070064635No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG18970.22567723990906288No Hit
CCTTATGGTGTACAGTGGTATCAGCAGCTTCCAGAAACAGCCCCCAAACTCCTCATCTTTGCTAGCAGCGATCGG18270.2173496664806842No Hit
GTAATAGACGGCCGTGTCGTCAGAATTCAGGCTACTCAGATCCATGTAGGTTGTGCTGACGGACGTGTCCCTGGT16490.19617383690566403No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA15580.18534799144877173No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC15320.1822548927468025No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15260.18154110073865576No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14300.1701204286083078No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14020.16678939923695635No Hit
CCATAAGGTGCCCCGATGTTGGAGCTAGTCCCATTGCAGGAGATGGTGACCTTCTGCCCTGGGGCCCCAGAC13980.16631353789819184No Hit
GTGTACAGTGGTATCAGCAGCTTCCAGAAACAGCCCCCAAACTCCTCATC13190.156915276457593No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC13070.15548769244129954No Hit
ATGGTGTACAGTGGTATCAGCAGCTTCCAGAAACAGCCCCCAAACTCCTCATCTTTGCTAGCAGCGATCGGCCCT12990.15453596976377051No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT12730.1514428710618013No Hit
GTCTATTACTGTGCGAGAGACTTAGCGCCGGTGTCCGAGATACTGACCCG12290.14620839633539182No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG12180.14489977765378945No Hit
ACCTTATGGTGTACAGTGGTATCAGCAGCTTCCAGAAACAGCCCCCAAAC12090.14382908964156935No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11980.14252047095996698No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCAGCTCAGCCTCCCTGGCCATCACTGGGCTCC11820.140617025604909No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC11640.13847564958046873No Hit
CACCAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11380.1353825508784995No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGCTTATCACTGCCAGT11080.13181359083776578No Hit
GTCCTATGACAACAGCCTGGGTGGCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG10970.1305049721561634No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA10700.12729290811950306No Hit
GGGCAGAAGGTCACCATCTCCTGCAATGGGACTAGCTCCAACATCGGGGCACCTTATGGTGTACAGTGGTATC10620.12634118544197406No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10590.1259842894379007No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC10540.12538946276444507No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGA10510.1250325667603717No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10440.12419980941753384No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCACCCAGGCTGTTGTCATAGGACTGGCAGTGATA10260.1220584333930936No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG10240.12182050272371134No Hit
GCTTATCACTGCCAGTCCTATGACAACAGCCTGGGTGGCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCT10060.1196791266992711No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC10050.11956016136457996No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT10050.11956016136457996No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9930.11813257734828647No Hit
CTCCTGCAATGGGACTAGCTCCAACATCGGGGCACCTTATGGTGTACAGTGGTATCAGCAGCTTCCAGAAACAGC9870.11741878534013975No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9380.11158948394027464No Hit
GTCATAGGACTGGCAGTGATAAGCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGCTGCC9350.11123258793620126No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9350.11123258793620126No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC9310.11075672659743678No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG9240.1099239692545989No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9240.1099239692545989No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT9130.10861535057299654No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9110.10837741990361428No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC9070.10790155856484979No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGCTTATCACTGCCAGTCCTAT9020.10730673189139417No Hit
GTGTCTGGGGCCCCAGGGCAGAAGGTCACCATCTCCTGCAATGGGACTAG8950.1064739745485563No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGG8910.10599811320979181No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC8900.10587914787510068No Hit
GTACAGTGGTATCAGCAGCTTCCAGAAACAGCCCCCAAACTCCTCATCTT8870.1055222518710273No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8780.10445156385880718No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCAACGG8690.10338087584658706No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8660.10302397984251369No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8480.10088260381807346No Hit
GGATGAGGCTGCTTATCACTGCCAGTCCTATGACAACAGCCTGGGTGGCTGGGTGTTCGGCGGAGGGACCA8410.10004984647523558No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)