FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972696
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46430.4773331030455559No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT41360.42520993198286No Hit
CTCCATCAACAGTCTACAACCTGAAGATTCTGCCACTTACTACTGTCAGC33470.3440951746486055No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30130.30975762211420627No Hit
GTTACAACACTCCGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAA28450.2924860388034905No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27770.2854951598443913No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27140.27901831610287287No Hit
GTAGGAGACAGAGTCACCATCTCTTGCCGGGCAGGTCAAACCATTATTAG26740.2749060343622262No Hit
GTCTACAACCTGAAGATTCTGCCACTTACTACTGTCAGCAGAGTTACAACACTCCGTACAGTTTTGGCCAGGGG26700.2744948061881616No Hit
GTTGATGGAGAGAGTGAAATCTGTCCCAGATCCACTGCCATTGAACCTCGATGGGACCCCACTTTGCAAACTGGA26580.27326112166596755No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG26550.2729527005354191No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT25560.2627748032273187No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25410.26123269757457623No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG24910.25609234539876796No Hit
GAGTTACAACACTCCGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT24460.2514660284405405No Hit
GTGTTGTAACTCTGCTGACAGTAGTAAGTGGCAGAATCTTCAGGTTGTAGACTGTTGATGGAGAGAGTGAAAT23930.24601725513418377No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG23670.24334427200276343No Hit
GGCCAAAACTGTACGGAGTGTTGTAACTCTGCTGACAGTAGTAAGTGGCA22390.2301849704326943No Hit
GTCTCCATCCTCCCTGTCTGCCTCTGTAGGAGACAGAGTCACCATCTCTT22080.22699795208369314No Hit
GTTTAGGGGCTGCCCCTGATTTCTGCTGATACCAATTTACATAACTAATAATGGTTTGACCTGCCCGGCAAG21990.22607268869204766No Hit
CCCCTAAACTCCTCATCTATGCTGCTTCCAGTTTGCAAAGTGGGGTCCCA21430.2203154942551424No Hit
CATCTATGCTGCTTCCAGTTTGCAAAGTGGGGTCCCATCGAGGTTCAATGGCAGTGGATCTGGGACAGATTTC20990.21579198434043112No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA20820.21404426460065631No Hit
GTTGTAACTCTGCTGACAGTAGTAAGTGGCAGAATCTTCAGGTTGTAGACTGTTGATGGAGAGAGTGAAAT20630.21209093077384916No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20200.20767022790265408No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG20010.20571689407584692No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA19990.20551127998881458No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAAATGTGACATCC19930.20489443772771762No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC18280.18793127554755032No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18260.18772566146051797No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC18210.18721162624293713No Hit
CTGTAGGAGACAGAGTCACCATCTCTTGCCGGGCAGGTCAAACCATTATTAGTTATGTAAATTGGTATCAGCAGA18120.18628636285129166No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17780.18279092337174205No Hit
CTGCTACTCTGGCTCCGAGGTGCCAAATGTGACATCCAAATGACCCAGTCTCCATCCTCCCTGTCTGCCTCTGT16950.1742579387599003No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTGTACGGAGTGTTGTAACTCTGCTGAC16520.1698372358887052No Hit
GCCCCTAAACTCCTCATCTATGCTGCTTCCAGTTTGCAAAGTGGGGTCCCATCGAGGTTCAATGGCAGTGGAT16300.16757548093134958No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC16240.16695863867025257No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC15940.1638744273647676No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAAATGTGACAT15900.16346319919070296No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15820.16264074284257363No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15590.16027618084170184No Hit
GATTTCACTCTCTCCATCAACAGTCTACAACCTGAAGATTCTGCCACTTACTACTGTCAGCAGAGTTACAACACT15290.15719196953621686No Hit
GGTCAAACCATTATTAGTTATGTAAATTGGTATCAGCAGAAATCAGGGGCAGCCCCTAAACTCCTCATCTATG15040.15462179344831273No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCATTGAACCTCGATGGGACCCCACTTTGCAAACTGGAAGCAGCAT14990.1541077582307319No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14750.15164038918634393No Hit
GTAGTAAGTGGCAGAATCTTCAGGTTGTAGACTGTTGATGGAGAGAGTGA14270.146705651097568No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13770.14156529892175973No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT13750.14135968483472738No Hit
ACTTACTACTGTCAGCAGAGTTACAACACTCCGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA13470.13848108761627476No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13320.1369389819635323No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13310.13683617492001612No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13220.13591091152837062No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13050.13416319178859581No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC13010.13375196361453118No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAAATGTGACATCCAAATGACCCAGTCTCCATCCTCC12810.13169582274420785No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC12490.12840599735169056No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCA12110.12449932969807627No Hit
GTTGTAGACTGTTGATGGAGAGAGTGAAATCTGTCCCAGATCCACTGCCA12100.12439652265456011No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12090.12429371561104394No Hit
CAGTAGTAAGTGGCAGAATCTTCAGGTTGTAGACTGTTGATGGAGAGAGT11900.12234038178423681No Hit
AAACTGTACGGAGTGTTGTAACTCTGCTGACAGTAGTAAGTGGCAGAATCTTCAGGTTGTAGACTGTTGATGG11880.12213476769720447No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT11850.12182634656665597No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC11740.12069546908797817No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT11700.12028424091391349No Hit
CCATTATTAGTTATGTAAATTGGTATCAGCAGAAATCAGGGGCAGCCCCTAAACTCCTCATCTATGCTGCTTCC11130.11442423943349206No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11040.11349897604184657No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC10990.11298494082426576No Hit
GTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC10960.11267651969371725No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10950.11257371265020108No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10760.11062037882339396No Hit
CCATCCTCCCTGTCTGCCTCTGTAGGAGACAGAGTCACCATCTCTTGCCG10750.11051757177987778No Hit
CTCCTCATCTATGCTGCTTCCAGTTTGCAAAGTGGGGTCCCATCGAGGTT10740.11041476473636162No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC10720.11020915064932929No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10670.10969511543174847No Hit
GCATAGATGAGGAGTTTAGGGGCTGCCCCTGATTTCTGCTGATACCAATTTACATAACTAATAATGGTTTGAC10610.10907827317065145No Hit
GTGGGGTCCCATCGAGGTTCAATGGCAGTGGATCTGGGACAGATTTCACTCTCTCCATCAACAGTCTACAACCTG10590.10887265908361914No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA10570.1086670449965868No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC10550.10846143090955447No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAA10480.10774178160494131No Hit
CAATAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGCCTCAGGGA10400.106919325256812No Hit
GTTCAATGGCAGTGGATCTGGGACAGATTTCACTCTCTCCATCAACAGTCTACAACCTGAAGATTCTGCCACTT10310.10599406186516652No Hit
ATAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGCCTCAGGGAGA10270.10558283369110184No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10100.10383511395132704No Hit
ATCTGGGACAGATTTCACTCTCTCCATCAACAGTCTACAACCTGAAGATT10090.10373230690781086No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC10070.10352669282077853No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10040.10321827169023003No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAAATGTGACATCCAAATG10010.10290985055968155No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10000.10280704351616538No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9840.10116213081990673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)