FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972696
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45690.4697253818253596No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT35550.3654790396999679No Hit
CTCCATCAACAGTCTACAACCTGAAGATTCTGCCACTTACTACTGTCAGC35000.35982465230657884No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28170.2896074415850379No Hit
GTTGATGGAGAGAGTGAAATCTGTCCCAGATCCACTGCCATTGAACCTCGATGGGACCCCACTTTGCAAACTGGA25700.26421410183654503No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG25530.2624663820967702No Hit
GTTACAACACTCCGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAA25470.2618495398356732No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25440.2615411187051247No Hit
GTCTACAACCTGAAGATTCTGCCACTTACTACTGTCAGCAGAGTTACAACACTCCGTACAGTTTTGGCCAGGGG24840.2553726960941548No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24760.2545502397460255No Hit
GTGTTGTAACTCTGCTGACAGTAGTAAGTGGCAGAATCTTCAGGTTGTAGACTGTTGATGGAGAGAGTGAAAT24310.24992392278779801No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24270.24951269461373338No Hit
GTTTAGGGGCTGCCCCTGATTTCTGCTGATACCAATTTACATAACTAATAATGGTTTGACCTGCCCGGCAAG23870.24540041287308675No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA23720.24385830722034427No Hit
GTAGGAGACAGAGTCACCATCTCTTGCCGGGCAGGTCAAACCATTATTAG23490.24149374521947245No Hit
GAGTTACAACACTCCGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT23360.2401572536537623No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT22660.23296076060763077No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG22020.22638110982259618No Hit
GGCCAAAACTGTACGGAGTGTTGTAACTCTGCTGACAGTAGTAAGTGGCA21020.21610040547097964No Hit
GTTGTAACTCTGCTGACAGTAGTAAGTGGCAGAATCTTCAGGTTGTAGACTGTTGATGGAGAGAGTGAAAT20830.2141470716441725No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20790.21373584347010782No Hit
CCCCTAAACTCCTCATCTATGCTGCTTCCAGTTTGCAAAGTGGGGTCCCA20660.21239935190439768No Hit
CATCTATGCTGCTTCCAGTTTGCAAAGTGGGGTCCCATCGAGGTTCAATGGCAGTGGATCTGGGACAGATTTC20540.2111656673822037No Hit
GTCTCCATCCTCCCTGTCTGCCTCTGTAGGAGACAGAGTCACCATCTCTT20450.21024040399055818No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18930.19461373337610108No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG18520.19039864459193828No Hit
CTGTAGGAGACAGAGTCACCATCTCTTGCCGGGCAGGTCAAACCATTATTAGTTATGTAAATTGGTATCAGCAGA18200.187108819199421No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18140.18649197693832398No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC17570.18063197545790258No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17420.1790898698051601No Hit
CTGCTACTCTGGCTCCGAGGTGCCAAATGTGACATCCAAATGACCCAGTCTCCATCCTCCCTGTCTGCCTCTGT17180.17662250076077213No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC16840.17312706128122252No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAAATGTGACATCC16670.17137934154144768No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG16170.1662389893656394No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15790.16233232171202513No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA15690.16130425127686346No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTGTACGGAGTGTTGTAACTCTGCTGAC15430.15863126814544318No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC15330.15760319771028153No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15110.15534144275292588No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC15090.15513582866589357No Hit
GCCCCTAAACTCCTCATCTATGCTGCTTCCAGTTTGCAAAGTGGGGTCCCATCGAGGTTCAATGGCAGTGGAT14950.15369653005666725No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14650.15061231875118228No Hit
GATTTCACTCTCTCCATCAACAGTCTACAACCTGAAGATTCTGCCACTTACTACTGTCAGCAGAGTTACAACACT14640.15050951170766613No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14450.14855617788085898No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAAATGTGACAT14410.14814494970679432No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14210.14608880883647102No Hit
GGTCAAACCATTATTAGTTATGTAAATTGGTATCAGCAGAAATCAGGGGCAGCCCCTAAACTCCTCATCTATG14190.14588319474943867No Hit
GTAGTAAGTGGCAGAATCTTCAGGTTGTAGACTGTTGATGGAGAGAGTGA14130.1452663524883417No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCATTGAACCTCGATGGGACCCCACTTTGCAAACTGGAAGCAGCAT13820.14207933413934057No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT13400.1377614383116616No Hit
GTGGGGTCCCATCGAGGTTCAATGGCAGTGGATCTGGGACAGATTTCACTCTCTCCATCAACAGTCTACAACCTG13080.1344716129191443No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12880.132415472048821No Hit
ACTTACTACTGTCAGCAGAGTTACAACACTCCGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA12540.12892003256927137No Hit
GTTGTAGACTGTTGATGGAGAGAGTGAAATCTGTCCCAGATCCACTGCCA12490.12840599735169056No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC12420.1276863480470774No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12340.12686389169894807No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT12080.12419090856752779No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11780.12110669726204282No Hit
CTCCTCATCTATGCTGCTTCCAGTTTGCAAAGTGGGGTCCCATCGAGGTT11760.1209010831750105No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC11580.11905055639171952No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAAATGTGACATCCAAATGACCCAGTCTCCATCCTCC11460.11781687186952552No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC11290.1160691521297507No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11160.11473266056404056No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCA11100.11411581830294358No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC11060.11370459012887892No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC11010.11319055491129809No Hit
AAACTGTACGGAGTGTTGTAACTCTGCTGACAGTAGTAAGTGGCAGAATCTTCAGGTTGTAGACTGTTGATGG10880.11185406334558792No Hit
CCATTATTAGTTATGTAAATTGGTATCAGCAGAAATCAGGGGCAGCCCCTAAACTCCTCATCTATGCTGCTTCC10880.11185406334558792No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10750.11051757177987778No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10450.10743336047439281No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10350.10640529003923117No Hit
GTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC10330.10619967595219884No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA10300.10589125482165034No Hit
GCATAGATGAGGAGTTTAGGGGCTGCCCCTGATTTCTGCTGATACCAATTTACATAACTAATAATGGTTTGAC10300.10589125482165034No Hit
CAGTAGTAAGTGGCAGAATCTTCAGGTTGTAGACTGTTGATGGAGAGAGT10240.10527441256055335No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10170.1045547632559402No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10100.10383511395132704No Hit
GTTCAATGGCAGTGGATCTGGGACAGATTTCACTCTCTCCATCAACAGTCTACAACCTGAAGATTCTGCCACTT10090.10373230690781086No Hit
GTCTCTGGTAACTCCATCAGTAGTGGTGTTTACCAGTGGAATTGGATCCG9940.10219020125506839No Hit
ATAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGCCTCAGGGAGA9890.10167616603748755No Hit
GTCCCAGATCCACTGCCATTGAACCTCGATGGGACCCCACTTTGCAAACTGGAAGCAGCATAGATGAGGAGTTT9850.1012649378634229No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC9780.10054528855880975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)