FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245631
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC54640.438653180596822No Hit
GCCTTGGGCTGACCTTGGACGGTCAGCCTGGTCCCCCCGCCGAATACCAC50900.4086282374154144No Hit
GTCGATAACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAACAAC45140.3623866136921769No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT44150.35443883461474546No Hit
CTCCTGGACAGTCGATAACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGT40560.32561810038446376No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATAACCATCTCCTG39720.3188745302581583No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA38190.3065915989566734No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC36820.2955931572030561No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTGGACGGTCAGCCTGGTCCCCC33320.26749494834345No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32260.25898520508882644No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGCTGACTATTACTGCAGCTCATAT31880.25593454241264063No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT31510.2529641603331966No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC29610.23771084695226757No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT28730.23064615443899517No Hit
GTCCAAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28470.22855885892371017No Hit
GACTATTACTGCAGCTCATATACCAGCACTCGTGTGGTATTCGGCGGGGGGACCAGGCTGACCGTCCAAGGTC28190.22631100221494166No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG27880.22382230371594797No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC26650.213947790316715No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG26580.21338582613952287No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAG26520.2129041425590725No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26010.20880983212524412No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC25820.20728450078715127No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATAAC25480.20455496049793237No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25000.20070149185432923No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24240.19460016650195763No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23740.19058613666487106No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG23710.19034529487464588No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC23110.18552845907014195No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA22970.18440453071575771No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATAACCATCT22740.1825580769906979No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC22370.17958769491125381No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22150.1778215217829357No Hit
CTATTACTGCAGCTCATATACCAGCACTCGTGTGGTATTCGGCGGGGGGACCAGGCTGACCGTCCAAGGTCAGCC22020.1767778740252932No Hit
GTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTCTGATGTCAGTAATCGGCCCTC21410.17188075762404756No Hit
ACCTTGGACGGTCAGCCTGGTCCCCCCGCCGAATACCACACGAGTGCTGGTATATGAGCTGCAGTAATAGTCAGC21070.16915121733482869No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20860.16746532480325232No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC20570.16513718749774212No Hit
GGTATATGAGCTGCAGTAATAGTCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCC20180.16200624422481458No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG20030.16080203527368858No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG19760.15863445916166186No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19610.15743025021053586No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC19560.1570288472268272No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19370.15550351588873432No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19110.15341622037344926No Hit
GCAGTAATAGTCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC18850.15132892485816427No Hit
GTATTCGGCGGGGGGACCAGGCTGACCGTCCAAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC18670.1498838741168131No Hit
GCCGAATACCACACGAGTGCTGGTATATGAGCTGCAGTAATAGTCAGCCT18410.14779657860152806No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATAAC18070.1450670383123092No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA17980.14434451294163358No Hit
GGTTATAAGTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTCTGATGTCAGTAAT17890.143621987570958No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17770.14265862041005722No Hit
GGCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC17690.14201637563612338No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC17600.1412938502654478No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16880.13551364730004312No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16520.13262354581734076No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16460.13214186223689037No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATAACCATCTCCTGCACTGGAACC16180.12989400552812191No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT15830.12708418464216129No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15590.12515745032035971No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15580.125077169723618No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGAC15490.1243546443529424No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTGGACGGTCAGC15410.12371239957900855No Hit
GCCTGGTCCCCCCGCCGAATACCACACGAGTGCTGGTATATGAGCTGCAGTAATAGTCAGCCTCGTCCTCAGG15130.12146454287024007No Hit
CCCCTGAGGGCCGATTACTGACATCAGAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGG14910.11969836974192195No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14880.11945752795169677No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCCCAGGACCCTGTGCCCTGAGTGAGG14720.11817303840382908No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTCTGATGTCAGTAATCGGCCCTCAGGGGTTTCTAATCG14660.11769135482337867No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC14490.11632658467876925No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA14490.11632658467876925No Hit
GACCATCTCTGGGCTCCAGCCTGAGGACGAGGCTGACTATTACTGCAGCTCATATACCAGCACTCGTGTGGTATT14430.11584490109831885No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG14270.11456041155045113No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14230.1142392891634842No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14170.11375760558303381No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC14140.11351676379280863No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT14050.11279423842213304No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13770.11054638171336456No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC13650.10958301455246378No Hit
GACCTTGGACGGTCAGCCTGGTCCCCCCGCCGAATACCACACGAGTGCTGGTATATGAGCTGCAGTAATAGTC13590.10910133097201337No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGATTACTG13460.10805768321437087No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13400.10757599963392049No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13350.10717459665021181No Hit
CCACACGAGTGCTGGTATATGAGCTGCAGTAATAGTCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGG13340.1070943160534701No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGT13320.10693375485998663No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC13220.1061309488925693No Hit
GTTATAAGTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAA13140.10548870411863545No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAG13100.10516758173166853No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTGGACGGTCAGCCTGGTCCCCCCGCCGAATACCACACGAGTG13070.10492673994144333No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCAC12840.1030802862163835No Hit
CTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTTATCGACTGTCCAGGAGACCCAGACACGG12780.10259860263593312No Hit
GGCAAAGCCCCCAAACTCATGATTTCTGATGTCAGTAATCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCC12770.10251832203919137No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12730.10219719965222444No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC12700.10195635786199926No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG12680.1017957966685158No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTAC12660.10163523547503234No Hit
GGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGATTACTGACATC12600.10115355189458194No Hit
GGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTCTGATGTCAGTAATCGGCCCTCAGGGGTTTCTA12580.10099299070109849No Hit
CTCGTGTGGTATTCGGCGGGGGGACCAGGCTGACCGTCCAAGGTCAGCCC12580.10099299070109849No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT12570.10091271010435675No Hit
GGACCAGGCTGACCGTCCAAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGG12530.10059158771738982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)