FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245631
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC52020.41761966425048824No Hit
GCCTTGGGCTGACCTTGGACGGTCAGCCTGGTCCCCCCGCCGAATACCAC43940.3527529420831691No Hit
GTCGATAACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAACAAC43060.34568824956989674No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42390.34030944958820064No Hit
CTCCTGGACAGTCGATAACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGT40960.3288293242541331No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC39910.3203998615962512No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATAACCATCTCCTG39560.3175900407102906No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA38670.3104450676002765No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGCTGACTATTACTGCAGCTCATAT31000.24886984989936828No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTGGACGGTCAGCCTGGTCCCCC29920.24019954545126126No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28780.23104755742270386No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC28720.23056587384225344No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28700.23040531264877No Hit
GACTATTACTGCAGCTCATATACCAGCACTCGTGTGGTATTCGGCGGGGGGACCAGGCTGACCGTCCAAGGTC27750.22277865595830545No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT27600.2215744470071795No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATAAC27230.21860406492773543No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC27070.21731957537986774No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26310.2112182500274961No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26170.21009432167311187No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG25520.2048760828848993No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25370.20367187393377334No Hit
GTCCAAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25120.20166485901523004No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC24890.19981840529017023No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24140.19379736053454033No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC23810.19114810084206318No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAG23010.18472565310272465No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG22660.18191583221676405No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATAACCATCT22020.1767778740252932No Hit
CTATTACTGCAGCTCATATACCAGCACTCGTGTGGTATTCGGCGGGGGGACCAGGCTGACCGTCCAAGGTCAGCC21980.17645675163832628No Hit
GTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTCTGATGTCAGTAATCGGCCCTC21390.1717201964305641No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21210.17027514568921293No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20950.16818785017392793No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC20760.16666251883583502No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20360.16345129496616573No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20130.1616048412411059No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA19970.1603203516932382No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19950.16015979049975473No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC19870.1595175457258209No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19510.15662744424311856No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC19450.15614576066266816No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG19170.15389790395389966No Hit
GGTATATGAGCTGCAGTAATAGTCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCC19130.15357678156693275No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG19030.15277397559951542No Hit
GTATTCGGCGGGGGGACCAGGCTGACCGTCCAAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC18930.1519711696320981No Hit
ACCTTGGACGGTCAGCCTGGTCCCCCCGCCGAATACCACACGAGTGCTGGTATATGAGCTGCAGTAATAGTCAGC18850.15132892485816427No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18220.14627124726343516No Hit
GGTTATAAGTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTCTGATGTCAGTAAT17570.1410530084752226No Hit
GCAGTAATAGTCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC17350.1392868353469045No Hit
GGCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC17260.1385643099762289No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT17160.1377615040088116No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATAACCATCTCCTGCACTGGAACC17120.1374403816218447No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATAAC16790.1347911219293675No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16700.13406859655869194No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16550.13286438760756597No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA16400.13166017865644No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15980.12828839359328725No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15920.12780671001283686No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC15880.12748558762586992No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15840.127164465238903No Hit
CCCCTGAGGGCCGATTACTGACATCAGAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGG15530.12467576673990932No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGAC15490.1243546443529424No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT15370.12339127719204163No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC15350.12323071599855816No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA15110.12130398167675661No Hit
GCCGAATACCACACGAGTGCTGGTATATGAGCTGCAGTAATAGTCAGCCT14890.1195378085484385No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14560.11688854885596135No Hit
GCCTGGTCCCCCCGCCGAATACCACACGAGTGCTGGTATATGAGCTGCAGTAATAGTCAGCCTCGTCCTCAGG14500.11640686527551096No Hit
GACCATCTCTGGGCTCCAGCCTGAGGACGAGGCTGACTATTACTGCAGCTCATATACCAGCACTCGTGTGGTATT14420.11576462050157711No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTCTGATGTCAGTAATCGGCCCTCAGGGGTTTCTAATCG14160.1136773249862921No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCCCAGGACCCTGTGCCCTGAGTGAGG14090.11311536080909997No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGT13870.11134918768078188No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC13800.11078722350358974No Hit
GACCTTGGACGGTCAGCCTGGTCCCCCCGCCGAATACCACACGAGTGCTGGTATATGAGCTGCAGTAATAGTC13330.10701403545672836No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13280.1066126324730197No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTGGACGGTCAGC12940.10388309218380082No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT12790.10267888323267484No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC12780.10259860263593312No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG12720.10211691905548273No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTGGACGGTCAGCCTGGTCCCCCCGCCGAATACCACACGAGTG12700.10195635786199926No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12620.10131411308806541No Hit
GTTATAAGTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAA12620.10131411308806541No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC12620.10131411308806541No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)