FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814275
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACAGTGAGTTCTTGGCGTTGTCCCTGG29990.36830309170734704No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22210.27275797488563447No Hit
GCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC20690.2540910626017009No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCACTGGCTCACTCTCC20180.24782782229590739No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20180.24782782229590739No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18110.2224064351723926No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17060.20951152866046482No Hit
AGCCAGGACAGGCCCCTGTACTTGTCTTCTATGGTAAAAACAACCGGCCC16950.20816063369254859No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA16790.2061956955573977No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC16710.2052132264898222No Hit
GGATGAGACTGACTATTTCTGTTACTCCCGGGACAGCAGTGGTAACCATCCCGTGGTATTCGGCGGAGGGACCA16570.2034939056215652No Hit
CCCCTGTACTTGTCTTCTATGGTAAAAACAACCGGCCCTCAGGGATCCCA15640.19207270271100058No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTC15460.18986214730895581No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC14980.18396733290350312No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC14920.18323048110282153No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14670.18016026526664825No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC14660.1800374566332013No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT14450.17745847533081577No Hit
GTCTCATCCTCCGCCTGAGCCCCAGTGATGGACAAGGAAGCTGTGTTTCCTGAGCTGGAGCCAGAGAATCGGT14370.1764760062632403No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14010.1720548954591508No Hit
GTGTATTACTGTGCGAGAGTCCATAGCAGTGGCTGGTACGATCTTCTTGACTACTGGGGCCAGGGAATCCTGGTC13670.1678794019219551No Hit
TCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13350.16394952565165333No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG13000.1596512234810107No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12930.1587915630468822No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12830.1575634767124129No Hit
GGCCTGGACCCCTCACTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGG12550.15412483497589882No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12370.15191427957385403No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12320.15130023640661938No Hit
GTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGCTT11810.14503699610082588No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11710.14380890976635657No Hit
CTCCAGGGACAACGCCAAGAACTCACTGTTTCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATT11550.14184397163120568No Hit
GGTTACCACTGCTGTCCCGGGAGTAACAGAAATAGTCAGTCTCATCCTCCGCCTGAGCCCCAGTGATGGACAAGG11550.14184397163120568No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGG11230.13791409536090388No Hit
GACCTACGACGGTCAGCTTGGTCCCTCCGCCGAATACCACGGGATGGTTACCACTGCTGTCCCGGGAGTAACAGA11230.13791409536090388No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11210.13766847809401No Hit
GTCCATCACTGGGGCTCAGGCGGAGGATGAGACTGACTATTTCTGTTACTCCCGGGACAGCAGTGGTAACCATCC11120.13656320039298764No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11040.13558073132541218No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC10850.13324736728992048No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG10800.13263332412268583No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGA10720.13165085505511037No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10700.1314052377882165No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG10650.13079119462098185No Hit
GTGATGGACAAGGAAGCTGTGTTTCCTGAGCTGGAGCCAGAGAATCGGTC10580.12993153418685333No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10570.1298087255534064No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT10540.1294402996530656No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10520.12919468238617177No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10450.12833502195204324No Hit
GTGGTAACCATCCCGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCGTA10270.12612446654999845No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA10260.12600165791655155No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC10240.12575604064965767No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAA10200.12526480611586996No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10150.12465076294863528No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG10030.12317705934727212No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG9850.12096650394522734No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9710.11924718307697031No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC9470.11629977587424396No Hit
GTATTACTGTGCGAGAGTCCATAGCAGTGGCTGGTACGATCTTCTTGACTACTGGGGCCAGGGAATCCTGGTCAC9400.11544011544011544No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT9190.11286113413772988No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT9070.1113874305363667No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG9040.11101900463602592No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATTATGCAAGCTGGT8950.10991372693500354No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT8890.10917687513432195No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8870.10893125786742809No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8850.10868564060053422No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCG8810.1081944060667465No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATAACATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG8780.1078259801664057No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG8780.1078259801664057No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8770.10770317153295876No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8760.10758036289951184No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC8680.10659789383193638No Hit
CTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTACTTGTCTTCTATGGTAAAAACAACCGGCC8580.10536980749746706No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8400.10315925209542232No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC8340.10242240029474073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)