FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673214
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTCCAATTGCAGATACAGTGTATTCTTGGAATTGTCTCTGG30910.45914077841518447No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22210.32990995433844217No Hit
GTATTACTGTCAGCAGTATGGTAGCTCGCCGCTCACTTTCGGCGGAGGGA21080.31312480132617565No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG17720.26321496582067516No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17340.2575704010908864No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC16830.2499948010588015No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15000.2228117656495557No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT14900.2213263538785587No Hit
GTATATTACTGTGCGAAAAGAGATGCGCCAGGATATGGTGACTACGTGATGACGTTTGATTATTGGGGCCGGGGA14230.21137409501287854No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGG13260.19696560083420725No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTTAGCAACTATGGCATGACCTGGGTCCGCCAGGCTCCAGGGAGGGG12710.18879583609372352No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC12630.1876075066769259No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC11930.17720962427994663No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC10980.1630982124554748No Hit
GTATGGTAGCTCGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT10670.15849343596538396No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC10530.1564138594859881No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATC10350.15374011829819345No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT10120.15032367122490026No Hit
GTCTCCAGGCACCCTGTCTTTGTCGCCAGGGGAAAGAGCCACCCTCTCCT9840.14616451826610855No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGAAGTAACTTCTTAGCCTGGTACC9800.14557035355770973No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9670.1436393182554136No Hit
GCTTGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG9440.1402228711821204No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG9440.1402228711821204No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGA9420.13992578882792098No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9080.13487538880653105No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC8940.13279581232713522No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC8750.12997352996224082No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT8580.12744832995154587No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAG8460.12566583582634944No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT8330.12373480052405327No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG8310.12343771816985387No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC8200.12180376522175713No Hit
GTCTGGGGGAGGCCTGGAACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACTTTTAGCA8200.12180376522175713No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8040.11942710638816185No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC8000.11883294167976304No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACTTTTAGCAACTATGGC7990.11868440050266335No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT7980.11853585932556363No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC7930.11779315344006512No Hit
CTTCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGC7900.117347529908766No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7860.11675336520036719No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTCCAATTGCAGATA7840.11645628284616778No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGAA7820.11615920049196837No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG7730.11482232989807105No Hit
CCCTGAGGAGACGGTGACCAGAATTCCCCGGCCCCAATAATCAAACGTCATCACGTAGTCACCATATCCTGGCGC7680.11407962401257252No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG7560.11229712988737607No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT7520.11170296517897726No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATC7510.11155442400187757No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC7450.11066317693927935No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGCCAGAGCATGTTCACCTGCCGCGTGG7430.11036609458507994No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT7360.109326306345382No Hit
CTCCAGGCACCCTGTCTTTGTCGCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGAAGTA7340.10902922399118259No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA7300.10843505928278378No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAATGGAGTTTGGGCT7290.10828651810568408No Hit
GTATGTTGTCTCACCATTATTACTGATAGAAGAGACCCACTCCAGTCCCCTCCCTGGAGCCTGGCGGACCCAGGT7210.10709818868888643No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCC7190.10680110633468703No Hit
CTCCAGTCCCCTCCCTGGAGCCTGGCGGACCCAGGTCATGCCATAGTTGCTAAAAGTGAATCCAGAGGCTGCAC7090.10531569456369No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC6940.10308757690719444No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAGG6920.10279049455299503No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA6820.101305082781998No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC6750.10026529454230007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)