FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673214
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTCCAATTGCAGATACAGTGTATTCTTGGAATTGTCTCTGG28800.427798590047147No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23720.35233967208049743No Hit
GTATTACTGTCAGCAGTATGGTAGCTCGCCGCTCACTTTCGGCGGAGGGA18360.27272160115505617No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC17820.2647003775916722No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15940.23677463629692788No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG13610.20216454203269688No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT13380.19874809495940368No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTTAGCAACTATGGCATGACCTGGGTCCGCCAGGCTCCAGGGAGGGG13220.19637143612580843No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12910.19176665963571762No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC12380.18389397724943332No Hit
GTATATTACTGTGCGAAAAGAGATGCGCCAGGATATGGTGACTACGTGATGACGTTTGATTATTGGGGCCGGGGA12330.1831512713639348No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGG11990.17810087134254488No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11440.16993110660206115No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC11400.16933694189366233No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11090.16473216540357152No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC10380.15418574182949257No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGAAGTAACTTCTTAGCCTGGTACC10340.15359157712109375No Hit
GTATGGTAGCTCGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT10200.1515120006416979No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10180.15121491828749847No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATC10060.14943242416230204No Hit
GTCTCCAGGCACCCTGTCTTTGTCGCCAGGGGAAAGAGCCACCCTCTCCT9690.143936400609613No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT9250.13740058881722603No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT9240.13725204764012633No Hit
GCTTGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG9050.13442976527523193No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC8360.12418042405535239No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG8290.12314063581565446No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGAA8290.12314063581565446No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8270.12284355346145505No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC8240.12239792993015595No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8240.12239792993015595No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGA8100.12031835345076007No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA7940.11794169461716482No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT7770.11541649460646987No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC7670.11393108283547283No Hit
CTTCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGC7550.11214858871027639No Hit
CTCCAGGCACCCTGTCTTTGTCGCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGAAGTA7510.11155442400187757No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACTTTTAGCAACTATGGC7500.11140588282477785No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC7500.11140588282477785No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT7450.11066317693927935No Hit
GTCTGGGGGAGGCCTGGAACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACTTTTAGCA7380.10962338869958141No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTCCAATTGCAGATA7280.10813797692858437No Hit
GTATGTTGTCTCACCATTATTACTGATAGAAGAGACCCACTCCAGTCCCCTCCCTGGAGCCTGGCGGACCCAGGT7270.10798943575148466No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAGG7190.10680110633468703No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7120.10576131809498912No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC7120.10576131809498912No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACACACATGGAGGACGCATTCTGCTGGA6980.10368174161559326No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGCCAGAGCATGTTCACCTGCCGCGTGG6890.10234487102169591No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT6870.10204778866749652No Hit
CCCTGAGGAGACGGTGACCAGAATTCCCCGGCCCCAATAATCAAACGTCATCACGTAGTCACCATATCCTGGCGC6850.1017507063132971No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG6830.1014536239590977No Hit
CTCCAGTCCCCTCCCTGGAGCCTGGCGGACCCAGGTCATGCCATAGTTGCTAAAAGTGAATCCAGAGGCTGCAC6820.101305082781998No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAG6780.10071091807359919No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT6780.10071091807359919No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)