FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004717538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72
Sequences flagged as poor quality0
Sequence length29-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTATGCACATGAGCTAAAGCAGAAAGTCCGTTACAGCCATAACCATCCCCCCCCCCCAGGATCCCTCC11.3888888888888888No Hit
ATGCAGTACGAGACAAAATCTAGTGCAGAAGACAAATTTGTAAAGAAGCTAGACCAATGTTAAATGATTCTAC11.3888888888888888No Hit
GCCCAGTTCCCCCCCCGACCCCCAGGTGCCACCCCCC11.3888888888888888No Hit
GGAAACCATCCCATTCGTGCGCAGAGGTGGGTGCTAGGGCAGAAAAGTAAGTACAAGAAAGAATTGGAGTTATTG11.3888888888888888No Hit
CCGCTTGGCGGGTAAACCCACACATGTCAATGTGTCTGTTGTCATGGCGGCGGTGGACGGCACATGCT11.3888888888888888No Hit
GAAAAGTATTTTTAATTTTTAATAATTTTATTTTTGAGTGGAAGTTTTTATTGTAAATTTTTAAATATGACCAT11.3888888888888888No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG11.3888888888888888No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTT11.3888888888888888No Hit
GTGCTACACAGCTGTGGTCCCACTCGTATATGGAGGTGAGACCAAAATGAAGGAAACAGCCTTAACCCCATATGC11.3888888888888888No Hit
GTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTCGT11.3888888888888888No Hit
GGTGAAAGATGAGCTGTAGGACCGCAATAGGGGTAGGTCCCCAGTGGAAAAAGTGTAAGAGCCCAAATGATGG11.3888888888888888No Hit
GGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACT11.3888888888888888No Hit
GAACTGTGGCTACACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAG11.3888888888888888No Hit
GAGCAGCCTCTGGATTCACCTTCAGTAGTTACGACATGCACTGGGACCGCCAAGCTACAGGAAAAGGTCT11.3888888888888888No Hit
CTGTGACACTCTCCTGGCAGTTACCCGATTGGAGGGCGATATCCACCTTCCACTGTACTATGGCCTCTCTTGGAT11.3888888888888888No Hit
CCAAAGGAACCATAACTGATTTAATGAGCAATTCACAGTTTCACTGTACCGGCCGTGCGTACTTAGACATGCTGT11.3888888888888888No Hit
GTGCAGCGGTACACGAATCCCGGCCAGGATGTGGCTGTGCCCTGGCCAGTTCCGTCGACTCCGCCTGGCC11.3888888888888888No Hit
CTTCCCACCTAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCC11.3888888888888888No Hit
ATAGGGAACCTCTATTTATGAGCTTGTGAATAAACACAACTCTCTCAACTCAGAAAAAAAAAAAAAAAAAAAAA11.3888888888888888No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC11.3888888888888888No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGC11.3888888888888888No Hit
CTACTAAGCCAACCACCAAAAAAAAAACCAAAAAAAACAACAAACAAAAACAAAAAAAAAAAAAAAAAAAAAAA11.3888888888888888No Hit
GGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.3888888888888888No Hit
ACACCCAATCGGGTAAATCCAAGGAGAGTGACACAGAGCACGACACCAAGCACAGCACCAACAGCCTCAGCACC11.3888888888888888No Hit
ATTTTGGTCTCACCACCATATACGAGTGGGACCACAGCTGTGTAGCACTT11.3888888888888888No Hit
GTCGGAAACGGAGCAGGTCAAAACACCCGTGCACATCACTAGTGCGATCGCGCCTCTGAATAGCCAC11.3888888888888888No Hit
CACAAGTACAAAGTAAAATAGAACCACAAAATAATGAACTGCATGTTCATAACATACAAAAATCGCCGCCTACTC11.3888888888888888No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.3888888888888888No Hit
GGGGACGGCCCATTGCCAATCTGTCCCAGCCCCCCGGGGTCAGATGCAAAATACAAAAAGAGAACCACAAGTAC11.3888888888888888No Hit
GAGTACAATTTATATATTTTTTTTAATTTTTTTTTTGATAGAGATTTTT11.3888888888888888No Hit
CCCTTGGCACAGGCCAGGTCATCAAGGGCTGGGACCAGGGGCTGCTGGGG11.3888888888888888No Hit
GCTCCTGGTTCAACGAGGCCATTGACTTCATAGACTCCATCAAGAATGCTGGAGGAATGGTGTTTGTCCACTGCC11.3888888888888888No Hit
GGGTTAAGAAGCCAAAAGACACAGTAGCCACAAGCCTGGAGGCGCCCCAGCCCCTTCAGCAGCAGCAGGCGGT11.3888888888888888No Hit
GGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGG11.3888888888888888No Hit
GGGCTGTGCCGAGCCATGGAACCATGGGAAGACCTTCACTTGCACTGCTGCCTACCCCGAGTCCAAGACCCCGCT11.3888888888888888No Hit
ACCAATAGAAGGCCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTTAGATTAAAAGTTGT11.3888888888888888No Hit
AAGGGAGCACCGTGGAGAAGACACAGGCCCCTAGAGAATCTTCATAGGTACTC11.3888888888888888No Hit
TTGTAAAGTGAGATTTCTTGGTATTAGAGGCCTGGTATGGTCCATTTCAA11.3888888888888888No Hit
CTACTAGGAATCACAACACAATCATGAATGCAACCCACACTCAGAATATAGCATCAAAATAAAAAACTCA11.3888888888888888No Hit
GTGCGTCCGCAGACGGGGATGAAAGGAGG11.3888888888888888No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGAAGGTGCCGCCACAGATCGT11.3888888888888888No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT11.3888888888888888No Hit
NCCCCAGACTCTACCAACTGCACCTCACACTGGACACCATCAAAAAAGCCAACAAGGAAAACCCAGCGGAGCCAC11.3888888888888888No Hit
ATACCAGTGGCCATGGTGGATGCACCATAGATGCGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCT11.3888888888888888No Hit
CTACAGAATGATCATAGGTTCTCATCCCTCACCCCCCACCACGGGAGACT11.3888888888888888No Hit
ACCCAGACACCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATT11.3888888888888888No Hit
CAAAAATAATTTAAGGTGAAATCTCTAATATTTATAAAAGTAGCAAAATAAATGCATAATTAAAATATAT11.3888888888888888No Hit
GCTTATCGTTATGTGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCAACATCTGTCTTC11.3888888888888888No Hit
ATTATATGATCATACTCTAAGTATCAAAATCTGCCAATTAGCATGCTCACATGAAAGAGGTATGCTCTCTTAGG11.3888888888888888No Hit
AGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTTTTGGATGAA11.3888888888888888No Hit
GAGTATGAACATGAGGGTGATTTCTCGTGTGAATGAGGGATTTATAATATTAATGTGGAGGCAGAGTT11.3888888888888888No Hit
GGCTGTCTCAGAGCCACTGGGCCTGGATGATGCTGGGAACAGGAAGTCATCAAAGGGCTCGGTCGTCAGCTCCAT11.3888888888888888No Hit
CATCTGAATCACAAGACAAATAGAGAGATAAACATCACCTTGGAACCAGA11.3888888888888888No Hit
GACTTACAATATTAGTTTCAATTAAACTTCTATCAAACACTTCATAATCCTTCAAACAAACTTCTTTCATTCAT11.3888888888888888No Hit
GAAGAGAAGCTGCGCTGGGGCTTCCATGGTTCCGTCTGGGTACTAACTGA11.3888888888888888No Hit
CTTGGGGGGTGCTGTCGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGG11.3888888888888888No Hit
CCCCCAGCATATACAAACTGCACCTAACACTTGACACAATCTAAAATGGCCAAAAGGAAACCCCAGCGGAGCCAC11.3888888888888888No Hit
AAATCAAACTCAAGGAGTGAAAATCAGGAGGTGTAAGAAAATGTTACTCG11.3888888888888888No Hit
ACAACATCACAAACTGCCAACTCAAAAGAACTTTTACTTCACCATCTTACAAGAACATAAAAAAAAATCATG11.3888888888888888No Hit
GGTCTCGACGCAGCGTACTTTGGGGGGTCGTAGGGTGTGTGTGGTGGTAT11.3888888888888888No Hit
TTGCAAGGCCGCGGTGTTTGTGGTTTTGTTGTGGGTGTGTGGTGTGGGGTGGGGTGTGTTTTTGTGTGGG11.3888888888888888No Hit
CCCCCCGGCCGGGGCCGGAGAGGGGCTGCCCGGGGTGGTTATGAGCAGATAAATGCACGCATCCCCCCCG11.3888888888888888No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCT11.3888888888888888No Hit
GAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAG11.3888888888888888No Hit
CTGTAGCACTGTCTTCATCACAGATATTGTTCTGGGTAGCAGTAACTATC11.3888888888888888No Hit
GGGCAGGGCACAGTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTG11.3888888888888888No Hit
CCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAG11.3888888888888888No Hit
GAGGTGGTGCCCTGGCTGCGCTCCTGCCGCGAAGCCCAAGTCAGCTACTA11.3888888888888888No Hit
CTTATAAACATGTCACGCTGCCGACGAAGGCGTAGGTGTAGATCTCGGTGGTCGCCGTAGGATTAAAAAA11.3888888888888888Illumina RNA PCR Primer (96% over 26bp)
AAAATACAAATCAAACACAGACCACGCAACTACATCGTAACAACAACGTTCACGCCCAAAA11.3888888888888888No Hit
CTACAGGTCAAAATAATAATAATTAATATAAACAAAAGCATGAATACCCTGAAACATAAATAAAACTTAATT11.3888888888888888No Hit
ACGGGGAGAGCTTAAATTGGGTGTGTGGGAGTAGCGGGTGTGTTCCGGCAGGTGCCGGCGGTGGCGCGGGGTGAC11.3888888888888888No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)