FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197464
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT39230.32760901371565243No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA35930.30005077396898777No Hit
GACCTAGGACGGTGACCCTGGTCCCAGGTCCGAAGACATAAATGTTGCCGTCAGCATATGAGCTGCAGTAATAAT33070.27616696618854514No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC31470.26280539540228354No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG29030.2424289999532345No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG28600.23883807780442667No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC28360.23683384218648745No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28050.23424503784664924No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTCGTTATAATTATGTCTCCTGGT27220.22731372300127603No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC26620.22230313395642792No Hit
GATTATTACTGCAGCTCATATGCTGACGGCAACATTTATGTCTTCGGACCTGGGACCAGGGTCACCGTCCTAGGT24870.20768891590895425No Hit
ACCTAGGACGGTGACCCTGGTCCCAGGTCCGAAGACATAAATGTTGCCGTCAGCATATGAGCTGCAGTAATAATC23530.19649860037546013No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC23340.19491191384459158No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC22340.18656093210317803No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT21500.1795461074403907No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC20620.1721972435079468No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20460.17086108642932063No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTCGTTATAATTATGTCTCCTGGTATCAACAGCACCCAGGCAAAGC20190.16860632135913897No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG20080.1676877133675835No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT19980.16685261519344213No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC19880.1660175170193008No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG19610.16376275194911913No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19160.16000481016548307No Hit
GGTATCAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTTATGAGGTCACTAAACGGCCCTCAGGGGTCCCTG18870.15758302546047312No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCAGGTCCGAAGACATAAATGTTGC18790.15691494692116004No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18700.15616335856443284No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG18540.15482720148580667No Hit
GCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCAGGTCCGAAGACATAAATGTTGCCGTCAGCATATGAGCTG18370.1534075345897664No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTCGTTATAATTATGTCTCCTGGTATCAACAGC18200.15198786769372608No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17440.1456411215702518No Hit
CTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGCCGACGCCGAAAATTCACTGTTTCTGCAAATG16450.1373736496462524No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16250.1357034532979697No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCCATTTTAGAAGGTATCAAGTGTGAGGTGCAGCTGGTGGAGT15870.13253008023623256No Hit
GTCTGCGTAGTGTGTGCGAGCAGCACCCTTATCGATGTATGAAATCCACT15690.13102690352277813No Hit
GTTGATACCAGGAGACATAATTATAACGACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGACTGTC15650.1306928642531216No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC15350.12818756973069753No Hit
CTCCTGTGCAGCCTCTGGGTTCACGTTCGGTAGTTATGAATTCCACTGGGTCCGTCAGGCTCCAGGGAAGGGG15320.12793704027845512No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC15320.12793704027845512No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGCTGCCAGACTTGGAGCCAG15290.1276865108262127No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG15250.12735247155655618No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTG15200.1269349224694855No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGA15010.12534823593861694No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT14970.1250141966689604No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCCATTTTAGAAGGTATCA14870.12417909849481905No Hit
GGATGAGGCTGATTATTACTGCAGCTCATATGCTGACGGCAACATTTATGTCTTCGGACCTGGGACCAGGGTCAC14660.12242539232912221No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTG14650.12234188251170808No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14480.12092221561566777No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14440.12058817634601124No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14410.12033764689376883No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGG14220.11875096036290027No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC14100.11774884255393064No Hit
GTGCTGGGTTTTCCTTGTTGCCATTTTAGAAGGTATCAAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGC14050.11733129346685996No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT13990.11683023456237517No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACT13850.11566109711857726No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13490.11265474369166839No Hit
GAAATAAACACCCGTGTCTTCGGCTCTCAGGTTAGTCATTTGCAGAAACAGTGAATTTTCGGCGTCGGCTCTGG13330.11131858661304223No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG13240.11056699825631501No Hit
CCATTGTCCCTTGGCCCCAGATCTGAAAACCGTAGCAGTCATTGTCGTCT13190.11014944916924435No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT13090.10931435099510299No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12910.10781117428164855No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12880.10756064482940614No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12750.10647501720302238No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCC12680.10589044848112344No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12670.1058069386637093No Hit
CACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGCCGACGCCGAAAATTCACTGTTTCTGCAAATG12460.10405323249801246No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC12380.10338515395869939No Hit
GTCAGGGAGGCCGTGCTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC12380.10338515395869939No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACC12340.10305111468904285No Hit
CATTTATGTCTTCGGACCTGGGACCAGGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT12220.10204899688007323No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAGCACGGCCTCCCTGACCGTCTCTGGGCTCC12170.10163144779300254No Hit
CCCCTGAGGGCCGTTTAGTGACCTCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGATACCAGG12160.10154793797558842No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGCTGCCAGACTTGGAGCCAGAGAAG12080.10087985943627532No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12080.10087985943627532No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA12060.10071283980144705No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)