FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826379
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28990.350807559243398No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT23990.29030263353739627No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG21950.26561662384934753No Hit
ATATTACTGTCAGCAATATGATAATCTCCCGCTCACTTTCGGCGGAGGGA21840.2642855154838155No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18710.22640943199185845No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTGAAATCTGTCCCAGAGCCACTTCC18700.22628842214044645No Hit
CTCCTGTACAGCCTCTGGATTCAATTTCAATTACGCCTCGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG17800.21539753551336616No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT17590.21285632863371406No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA16880.2042646291834618No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16120.19506788047614956No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16120.19506788047614956No Hit
GACCAGGGCAATCCCCTAAGCTCCTGATCTACGATGCATCCATTTTGGGG16120.19506788047614956No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16000.19361576225920551No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG15510.18768627954001735No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCCACTATTTAAATTGGTATCAGCAG15210.18405598399765724No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13320.16118512208078858No Hit
GTAGTACACGGCTGTGTCGTCGGCTTTCAGGCTGTTCATTTGCAGATACAGCGTGTTTTTTGAGTCATCTCTCG13220.15997502356666857No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG12970.15694977728136847No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCC12920.15634472802430846No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCC12830.15525563936160042No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC12690.1535615014418324No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC12470.1508992847107683No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC12210.14775302857405623No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCC12180.1473899990198202No Hit
AGTCAGGACATTAGCCACTATTTAAATTGGTATCAGCAGAGACCAGGGCA12090.14630091035711218No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG11570.14000839808368798No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11460.13867728971815596No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA11160.13504699417579585No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTGAAATCTGTCCCAGAGCCACTTCC11110.13444194491873585No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGAGATTATCATATTGCTGAC11070.1339579055130878No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11060.1338368956616758No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGAGATT11050.1337158858102638No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10710.1296015508622557No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10690.1293595311594317No Hit
CCCTTAGACTCTCCTGTACAGCCTCTGGATTCAATTTCAATTACGCCTCG10580.12802842279389964No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC10450.12645529472554362No Hit
GGGCAATCCCCTAAGCTCCTGATCTACGATGCATCCATTTTGGGGACAGG10310.12476115680577556No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT10010.12113086126341545No Hit
GCAGAGACCAGGGCAATCCCCTAAGCTCCTGATCTACGATGCATCCATTTTGGGGACAGGGGTCCCATCAAGGTT9920.12004177260070742No Hit
CCCCTAAGCTCCTGATCTACGATGCATCCATTTTGGGGACAGGGGTCCCA9910.11992076274929542No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGGAGATTATCATATTGCTGACAGTAATATGTTGCAATATCTTCAGG9640.11665349676117133No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9550.1155644080984633No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCC9260.1120551224075152No Hit
GTACAATAGACTGAGGCACAGTAGTACACGGCTGTGTCGTCGGCTTTCAG9150.11072401404198316No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC9140.11060300419057116No Hit
GGTCTCTGCTGATACCAATTTAAATAGTGGCTAATGTCCTGACTCGCCTGGCAAGTGATGGTGACT9080.10987694508209914No Hit
GACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA9070.10975593523068713No Hit
GCCCTGGTCTCTGCTGATACCAATTTAAATAGTGGCTAATGTCCTGACTCGCCTGGCAAGTGATGGTGACT9060.10963492537927513No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG9030.10927189582503913No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT8980.1086668465679791No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8940.10818280716233107No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT8900.10769876775668308No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8670.10491554117420698No Hit
ACATATTACTGTCAGCAATATGATAATCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGCTCAAACGA8610.10418948206573496No Hit
GATCTACGATGCATCCATTTTGGGGACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGCTCTGGGACAGATTTC8430.1020113047403189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)