FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478817
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGCGTGACAACTGGCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGA72610.4910005768124116No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61880.4184425794401877No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGTTGAGAGTGAAGTCTGTCCCAGACCCACTGCC60680.41032798513947294No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT56880.3846317698538764No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC52520.35514874389461304No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40560.2742732873641566No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38640.26128993648301313No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG36580.2473598829334529No Hit
GTAATACACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGTTGAGAGTGAAGTCTGTCCCAGACCCACTGCC35040.236946153580869No Hit
GTGTATTACTGTCAGCAGCGTGACAACTGGCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGA34470.23309172128802955No Hit
GTCTGGGACAGACTTCACTCTCAACATCAGCAGCCTAGAGCCTGAAGATT34250.23160404566623188No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCTAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTGGCAC34170.23106307271285087No Hit
GTAATACACAGCCGTGTCCTCAAATCTCAGGCTGTCTATTTGTAGATACAGTGTGTTGTTGGAATTGTCTCTGG33930.22944015385270794No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT33750.22822296470760076No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAAGGGCCCTGTT33130.22403042431889814No Hit
GTCTTTGTCTCTAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTGGCACCTACGTAGCCTGGT31290.2115880463911356No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30920.20908604648174856No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG30310.2049611277122186No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA29780.20137718189606962No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA28410.19211302006942035No Hit
CTCCTGCAGGGCCAGTCAGAATGTTGGCACCTACGTAGCCTGGTACCAACAGAAGGCTGGCCAGGCTCCCAGGCT28040.18961102016003334No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG27870.18846145263409875No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA26490.17912966918827686No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC26360.17825058813903275No Hit
GCCTAGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGCGTGACAAC26220.17730388547061604No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC25720.1739228045119849No Hit
GTGTATTACTGTGCGATGCCCAGCAGTTTAAGGGAGATGGGTACAAACTT25150.17006837221914545No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25150.17006837221914545No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTGGCACCTACGTAGCCTGGTACCAACAGAAGGCTGGCCAGG24630.16655204802216908No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC24170.16344145354022843No Hit
CTAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTGGCAC24090.16290048058684747No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23960.16202139953760336No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCTAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTC23480.1587755618173175No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGGGGAGGCC23420.15836983210228175No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGGGGAGGCCAGTTGTCACGCTG20320.1374071301587688No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20040.13551372482193536No Hit
TCTATGATGCATCCAACAGGGCCCTTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT19880.1344317789151734No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC19420.1313211844332328No Hit
GTCAGAATGTTGGCACCTACGTAGCCTGGTACCAACAGAAGGCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG19340.1307802114798518No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18820.12726388728287544No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18670.12624956299528609No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18470.12489713061183363No Hit
ACAGAAGGCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCCTTGGCATCCCAGCCAGGTT18310.12381518470507169No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC18300.12374756308589907No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT17840.12063696860395844No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGACAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCT17790.12029886050809534No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17750.12002837403140483No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17560.11874356326712501No Hit
CATCTATGATGCATCCAACAGGGCCCTTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTC17320.11712064440698206No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17240.11657967145360108No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC17050.11529486068932125No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT16050.10853269877205901No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC16040.10846507715288639No Hit
CCCAGATACCACCGGACAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCTAGGGGACAGAGCCAC15760.10657167181605297No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15720.10630118533936249No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15710.10623356372018987No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15640.10576021238598149No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC15580.10535448267094576No Hit
ACGTAGCCTGGTACCAACAGAAGGCTGGCCAGGCTCCCAGGCTCCTCATC15520.10494875295591004No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCAACATCAGCAGCC15410.10420491514501118No Hit
GCCTGGTACCAACAGAAGGCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCCTTGGCATC15150.102446753046523No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15150.102446753046523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)