FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004717548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478817
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGCGTGACAACTGGCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGA69260.468347334389583No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60230.40728501227670494No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGTTGAGAGTGAAGTCTGTCCCAGACCCACTGCC56200.3800334997501381No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT49270.3331717176635108No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC48180.32580096117369495No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38490.2602756121954238No Hit
GTAATACACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGTTGAGAGTGAAGTCTGTCCCAGACCCACTGCC34490.23322696452637479No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34330.23214501861961284No Hit
GTGTATTACTGTCAGCAGCGTGACAACTGGCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGA33810.22862869442263647No Hit
GTAATACACAGCCGTGTCCTCAAATCTCAGGCTGTCTATTTGTAGATACAGTGTGTTGTTGGAATTGTCTCTGG33250.2248418837489696No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA33070.22362469460386242No Hit
GTCTGGGACAGACTTCACTCTCAACATCAGCAGCCTAGAGCCTGAAGATT31970.21618631649487396No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCTAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTGGCAC31340.21192615448699872No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31320.21179091124865346No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAAGGGCCCTGTT31170.21077658696106416No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT30960.2093565329584391No Hit
GTCTTTGTCTCTAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTGGCACCTACGTAGCCTGGT30420.20570496552311746No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29780.20137718189606962No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG29060.19650842531564078No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG26290.1777772368048244No Hit
CTCCTGCAGGGCCAGTCAGAATGTTGGCACCTACGTAGCCTGGTACCAACAGAAGGCTGGCCAGGCTCCCAGGCT26190.17710102061309818No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA26080.1763571828021993No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC24670.16682253449885956No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24550.1660110750687881No Hit
GTGTATTACTGTGCGATGCCCAGCAGTTTAAGGGAGATGGGTACAAACTT24360.16472626430450826No Hit
GCCTAGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGCGTGACAAC23560.15931653477069846No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC23490.15884318343649012No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC23240.15715264295717454No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC22450.1518105350425374No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGGGGAGGCC21950.14842945408390626No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG21690.14667129198541806No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC21400.14471026502941203No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA21350.1443721569335489No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGGGGAGGCCAGTTGTCACGCTG20890.14126156245160829No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20700.13997675168732845No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCTAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTC20610.13936815711477485No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20230.1367985355862152No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19820.13402604920013766No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTGGCACCTACGTAGCCTGGTACCAACAGAAGGCTGGCCAGG19380.13105069795654228No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGACAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCT19300.1305097250031613No Hit
CTAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTGGCAC18270.12354469822838121No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT18140.12266561717913711No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17310.11705302278780944No Hit
ACAGAAGGCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCCTTGGCATCCCAGCCAGGTT17110.11570059040435701No Hit
TCTATGATGCATCCAACAGGGCCCTTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT17030.11515961745097601No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC16850.1139424283058688No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16510.11164329325399965No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16500.11157567163482703No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC16210.10961464467882098No Hit
GTCAGAATGTTGGCACCTACGTAGCCTGGTACCAACAGAAGGCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG16050.10853269877205901No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15830.10704502315026132No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT15640.10576021238598149No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15200.10278486114238611No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15150.102446753046523No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC15110.1021762665698325No Hit
GCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGGGG15030.10163529361645153No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)