FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1343737
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGCACCAGTATTGTCCGCTGATTG77710.5783125715820878No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC66400.4941443154426796No Hit
GTCTTCATATAACATCAGCACAGGGGCCTGGCCTGGCTTCTGCTGATACCAATAAACATACTGCTTTGACAATGC57430.427390181263149No Hit
ATATGAAGACAGTGCGAGGCCCTCAGGGATCCCTGAGCGATTTTCTGGCT55960.4164505405447643No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT49070.36517562588512487No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC47340.3523010827267538No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGTCAAAGCAGTATGTTTATTGGTATCAGCAGA47140.3508126962344566No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA45040.33518463806533566No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC42260.3144960658224042No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG41100.30586342416708034No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG40180.2990168463025131No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCCGGAGCCAGA39060.2906818819456486No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCAC38120.2836864654318516No Hit
ATACTGGTGCTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTC36190.26932353578118334No Hit
GACTATTACTGTCAATCAGCGGACAATACTGGTGCTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGT35250.2623281192673864No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC34650.25786295979049473No Hit
GTGCTGATGTTATATGAAGACAGTGCGAGGCCCTCAGGGATCCCTGAGCGATTTTCTGGCTCCGGCTCAGGGACA34220.25466292883205566No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC33890.25220709111976525No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33680.25064428530285315No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC33630.25027218867977885No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG33240.24736983501979926No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC32970.245360513255198No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT32810.24416980406136024No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG32010.2382162580921713No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC31730.2361325170029552No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG31570.2349418078091174No Hit
CATCAGCACAGGGGCCTGGCCTGGCTTCTGCTGATACCAATAAACATACTGCTTTGACAATGCATCTCCAGAGC31550.2347929691598877No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA31370.2334534213168202No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC31280.23278364739528642No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGAC30080.22385332844150307No Hit
GTATTGTCCGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGAC30020.2234068124938139No Hit
GTGCGAGGCCCTCAGGGATCCCTGAGCGATTTTCTGGCTCCGGCTCAGGG29120.21670907327847638No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC29040.2161137186815575No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC28890.21499742881233458No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG28280.210457850010828No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT28210.20993691473852397No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGCACCAGTATTGTCCGCTGATTGACAGTAATAGT27690.20606710985855117No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCCGGAGCC27040.2012298537585852No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC26910.200262402538592No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG26660.19840191942322047No Hit
GGCCAGGCCCCTGTGCTGATGTTATATGAAGACAGTGCGAGGCCCTCAGG26140.19453211454324768No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25340.18857856857405877No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA24750.18418782842178194No Hit
CTATTACTGTCAATCAGCGGACAATACTGGTGCTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTC24710.1838901511233225No Hit
GCTCTGGAGATGCATTGTCAAAGCAGTATGTTTATTGGTATCAGCAGAAG24570.18284828057871444No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24280.18069012016488348No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC24040.1789040563741268No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGCACCAGTATTGTCCGCTGATTGACAGTAATAGTC23830.1773412505572147No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA23710.17644821866183635No Hit
GAATACCACAGCACCAGTATTGTCCGCTGATTGACAGTAATAGTCAGCCT23690.17629938001260664No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT22760.16937838282342452No Hit
TATGAAGACAGTGCGAGGCCCTCAGGGATCCCTGAGCGATTTTCTGGCTCCGGCTCAGGGACAACAGTCACGTTG22560.1678899963311273No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC22250.1655829972680666No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA22150.16483880402191797No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGATACCAATAAACATACTGCTTT22090.16439228807422882No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT21990.1636480948280802No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCG20880.15538754979583058No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20870.1553131304712157No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTC20840.15508987249737116No Hit
CCCCTGTGCTGATGTTATATGAAGACAGTGCGAGGCCCTCAGGGATCCCT20760.15449451790045224No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT20560.15300613140815503No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20560.15300613140815503No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC20310.15114564829278349No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC20060.14928516517741194No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC20050.1492107458527971No Hit
GGATCACCTGCTCTGGAGATGCATTGTCAAAGCAGTATGTTTATTGGTATCAGCAGAAGCCAGGCCAGGCCC19980.14868981058049305No Hit
GCCCTCAGGGATCCCTGAGCGATTTTCTGGCTCCGGCTCAGGGACAACAGTCACGTTGACCATCAGTGGAGTCC19240.1431827805589933No Hit
GTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG19160.14258742596207444No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA19070.1419176520405407No Hit
GGCCTGGCCTGGCTTCTGCTGATACCAATAAACATACTGCTTTGACAATGCATCTCCAGAGCAGGTGATCCTGGC19060.1418432327159258No Hit
ACGTGACTGTTGTCCCTGAGCCGGAGCCAGAAAATCGCTCAGGGATCCCTGAGGGCCTCGCACTGTCTTCATATA18810.1399827496005543No Hit
CCAGTATTGTCCGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTG18540.137973427835953No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18040.13425246160520993No Hit
CCACTGATGGTCAACGTGACTGTTGTCCCTGAGCCGGAGCCAGAAAATCGCTCAGGGATCCCTGAGGGCCTCGC17950.13358268768367618No Hit
TCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCC17950.13358268768367618No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG17950.13358268768367618No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT17800.1324663978144533No Hit
GCTTGGTCCCTCCGCCGAATACCACAGCACCAGTATTGTCCGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTG17770.1322431398406087No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17690.13164778524368978No Hit
GTATGTTTATTGGTATCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGATGTTATATGAAGACAGTGCGAGGCCCTC17530.13045707604985202No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17460.12993614077754798No Hit
GCACCAGTATTGTCCGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTG17430.12971288280370338No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC17370.12926636685601423No Hit
CACCAGTATTGTCCGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTG17340.12904310888216966No Hit
GTCAAAGCAGTATGTTTATTGGTATCAGCAGAAGCCAGGCCAGGCCCCTG17230.12822449631140617No Hit
AGCCAGGCCAGGCCCCTGTGCTGATGTTATATGAAGACAGTGCGAGGCCC17160.12770356103910216No Hit
ATAATAGACAGCCGTGTCCTCAGCTCTCAGACTGCTCATTTCAAGATACAGTGTCTTCTTCGAATTGTCTCTGG17150.12762914171448728No Hit
GCAGAAGCCAGGCCAGGCCCCTGTGCTGATGTTATATGAAGACAGTGCGA17120.1274058837406427No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC17080.12710820644218326No Hit
GTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCGGACAATACTGGTGCTGTGGTATTCGGCGGAGGG17070.1270337871175684No Hit
GTCCTGGGGACACTGACACCGAGGGTGGCTGTGTCAGCTCATAGGAGGCCTCAGAGCCTGTGCAGAGAGTGAGG17040.12681052914372382No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGG16850.12539656197604143No Hit
GTTTATTGGTATCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGATGTTATATGAAGACAGTGCGAGGCCCTCAGGG16660.12398259480835908No Hit
GGGACAACAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCGGAC16650.1239081754837442No Hit
CAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTAT16500.1227918856145213No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA16420.12219653101760239No Hit
GGAGATGCATTGTCAAAGCAGTATGTTTATTGGTATCAGCAGAAGCCAGG16270.1210802411483795No Hit
GGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGTCAAAGCAGTATGTTTATTGGTATCAGCAGAAGCC16150.12018720925300115No Hit
CTGTTGTCCCTGAGCCGGAGCCAGAAAATCGCTCAGGGATCCCTGAGGGCCTCGCACTGTCTTCATATAACATC16080.11966627398069711No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCGGAC16020.11921975803300794No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15870.11810346816378502No Hit
ACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCT15780.11743369424225127No Hit
CAGGGATCCCTGAGCGATTTTCTGGCTCCGGCTCAGGGACAACAGTCACGTTGACCATCAGTGGAGTCCAGGC15710.11691275896994725No Hit
GGCCTGGCTTCTGCTGATACCAATAAACATACTGCTTTGACAATGCATCTCCAGAGCAGGTGATCCTGGCCGT15450.11497785652996086No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15440.11490343720534599No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15310.11393598598535279No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAG15160.11281969611612987No Hit
GGGATCCCTGAGGGCCTCGCACTGTCTTCATATAACATCAGCACAGGGGC15100.1123731801684407No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15080.11222434151921098No Hit
CCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGAC14960.11133130962383264No Hit
GCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCA14850.11051269705306917No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC14840.1104382777284543No Hit
CCACAGCACCAGTATTGTCCGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCA14770.10991734245615027No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14690.10932198785923138No Hit
CAATACTGGTGCTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGT14600.10865221393769763No Hit
GGCTTCTGCTGATACCAATAAACATACTGCTTTGACAATGCATCTCCAGAGCAGGTGATCCTGGCCGTCTGTC14600.10865221393769763No Hit
GGACTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCCGGAGCCAGAAAATCGCTCAGGGATCCCTGAGGGC14440.10746150474385985No Hit
GGACAATACTGGTGCTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCC14410.10723824677001527No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCC14340.10671731149771123No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC14310.10649405352386665No Hit
AGCACATACTACGCAGATTCCGTGAAGGGCAGATTCACCATCTCCAGAGA14250.10604753757617748No Hit
GACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGTCAA14170.10545218297925858No Hit
GCCTCGCACTGTCTTCATATAACATCAGCACAGGGGCCTGGCCTGGCTTC14130.10515450568079913No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG14080.10478240905772483No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT14000.10418705446080595No Hit
CTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCCGGAGCCAGAAAATCGCTCAGGGATCCCTGAGGGCCTCG13900.10344286121465732No Hit
GTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATG13780.102549829319279No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13750.10232657134543442No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCAC13720.10210331337158983No Hit
GGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGG13530.10068934620390746No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA13490.10039166890544803No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)