FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1343737
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGCACCAGTATTGTCCGCTGATTG67900.5053072141349089No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC63410.4718929373828361No Hit
GTCTTCATATAACATCAGCACAGGGGCCTGGCCTGGCTTCTGCTGATACCAATAAACATACTGCTTTGACAATGC55780.4151109927016968No Hit
ATATGAAGACAGTGCGAGGCCCTCAGGGATCCCTGAGCGATTTTCTGGCT55250.41116676849710915No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA46520.3461986981083352No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT45280.33697070185609235No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGTCAAAGCAGTATGTTTATTGGTATCAGCAGA42850.31888680597468105No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC42200.31404954987471506No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC40340.30020755549635086No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG39680.29529588007177No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG38720.2881516249087433No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCAC38220.2844306586780002No Hit
GTGCTGATGTTATATGAAGACAGTGCGAGGCCCTCAGGGATCCCTGAGCGATTTTCTGGCTCCGGCTCAGGGACA38080.2833887881333922No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC33470.24908147948594106No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT33280.24766751231825873No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC33220.24722099637056955No Hit
ATACTGGTGCTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTC32660.24305351419213728No Hit
GACTATTACTGTCAATCAGCGGACAATACTGGTGCTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGT32600.24260699824444812No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC31690.23583483970449573No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC31690.23583483970449573No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30860.22965803576146226No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30630.22794639129532046No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC30190.22467194101226654No Hit
GTGCGAGGCCCTCAGGGATCCCTGAGCGATTTTCTGGCTCCGGCTCAGGG29630.2205044588338343No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG29410.21886723369230737No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT29350.2184207177446182No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC29170.21708116990155069No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCCGGAGCCAGA29090.2164858153046318No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29090.2164858153046318No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGAC28910.21514626746156426No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA28490.21202065582774013No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG28240.2101601727123686No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG27790.2068113031046998No Hit
GGCCAGGCCCCTGTGCTGATGTTATATGAAGACAGTGCGAGGCCCTCAGG27090.2016019503816595No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26660.19840191942322047No Hit
CATCAGCACAGGGGCCTGGCCTGGCTTCTGCTGATACCAATAAACATACTGCTTTGACAATGCATCTCCAGAGC26470.19698795225553808No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC26150.19460653386786253No Hit
GCTCTGGAGATGCATTGTCAAAGCAGTATGTTTATTGGTATCAGCAGAAG25780.19185301885711267No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGCACCAGTATTGTCCGCTGATTGACAGTAATAGT25620.19066230966327488No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG24420.18173199070949153No Hit
GTATTGTCCGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGAC24130.17957383029566054No Hit
CTATTACTGTCAATCAGCGGACAATACTGGTGCTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTC22710.16900628620035021No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT22650.16855977025266106No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC22370.16647602916344495No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21790.16215970833578297No Hit
CCCCTGTGCTGATGTTATATGAAGACAGTGCGAGGCCCTCAGGGATCCCT21470.15977828994810742No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21060.15672709763889808No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20930.15575964641890488No Hit
TATGAAGACAGTGCGAGGCCCTCAGGGATCCCTGAGCGATTTTCTGGCTCCGGCTCAGGGACAACAGTCACGTTG20930.15575964641890488No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT20720.15419684060199282No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC20570.15308055073276988No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20550.15293171208354017No Hit
GAATACCACAGCACCAGTATTGTCCGCTGATTGACAGTAATAGTCAGCCT20500.15255961546046584No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC20450.15218751883739154No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA20190.15025261639740514No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC20180.1501781970727903No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA19970.1486153912558782No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGATACCAATAAACATACTGCTTT19950.14846655260664846No Hit
GTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG19800.14735026273742555No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGCACCAGTATTGTCCGCTGATTGACAGTAATAGTC19600.1458618762451283No Hit
GGATCACCTGCTCTGGAGATGCATTGTCAAAGCAGTATGTTTATTGGTATCAGCAGAAGCCAGGCCAGGCCC19270.1434060385328379No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCCGGAGCC19170.1426618452866893No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTC19090.1420664906897704No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19060.1418432327159258No Hit
GGCCTGGCCTGGCTTCTGCTGATACCAATAAACATACTGCTTTGACAATGCATCTCCAGAGCAGGTGATCCTGGC19020.14154555541746636No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19010.1414711360928515No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18950.14102462014516234No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCG18620.1385687824328719No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC18580.13827110513441246No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA18030.1341780422805951No Hit
GCCCTCAGGGATCCCTGAGCGATTTTCTGGCTCCGGCTCAGGGACAACAGTCACGTTGACCATCAGTGGAGTCC17970.13373152633290591No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT17970.13373152633290591No Hit
AGCCAGGCCAGGCCCCTGTGCTGATGTTATATGAAGACAGTGCGAGGCCC17930.13343384903444647No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17610.1310524306467709No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCGGAC17450.12986172145293312No Hit
GTATGTTTATTGGTATCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGATGTTATATGAAGACAGTGCGAGGCCCTC17410.12956404415447367No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG17270.12852217360986562No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT17180.12785239968833187No Hit
ACGTGACTGTTGTCCCTGAGCCGGAGCCAGAAAATCGCTCAGGGATCCCTGAGGGCCTCGCACTGTCTTCATATA17180.12785239968833187No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17090.12718262576679812No Hit
TCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCC16860.12547098130065631No Hit
GTTTATTGGTATCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGATGTTATATGAAGACAGTGCGAGGCCCTCAGGG16640.12383375615912937No Hit
ATAATAGACAGCCGTGTCCTCAGCTCTCAGACTGCTCATTTCAAGATACAGTGTCTTCTTCGAATTGTCTCTGG16590.12346165953605504No Hit
GGGATCCCTGAGGGCCTCGCACTGTCTTCATATAACATCAGCACAGGGGC16580.1233872402114402No Hit
GTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCGGACAATACTGGTGCTGTGGTATTCGGCGGAGGG16520.12294072426375102No Hit
GGAGATGCATTGTCAAAGCAGTATGTTTATTGGTATCAGCAGAAGCCAGG16440.12234536966683213No Hit
GCAGAAGCCAGGCCAGGCCCCTGTGCTGATGTTATATGAAGACAGTGCGA16350.12167559574529838No Hit
GGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGTCAAAGCAGTATGTTTATTGGTATCAGCAGAAGCC16220.12070814452530516No Hit
CCACTGATGGTCAACGTGACTGTTGTCCCTGAGCCGGAGCCAGAAAATCGCTCAGGGATCCCTGAGGGCCTCGC16140.12011278992838628No Hit
CAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTAT16110.1198895319545417No Hit
GTCCTGGGGACACTGACACCGAGGGTGGCTGTGTCAGCTCATAGGAGGCCTCAGAGCCTGTGCAGAGAGTGAGG16040.11936859668223766No Hit
GGGACAACAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCGGAC16010.11914533870839307No Hit
CCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGAC15920.11847556478685935No Hit
CCAGTATTGTCCGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTG15660.11654066234687295No Hit
GCTTGGTCCCTCCGCCGAATACCACAGCACCAGTATTGTCCGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTG15630.11631740437302836No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15550.11572204977610945No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA15550.11572204977610945No Hit
ACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCT15500.11534995315303515No Hit
GCACCAGTATTGTCCGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTG15470.11512669517919057No Hit
GTCAAAGCAGTATGTTTATTGGTATCAGCAGAAGCCAGGCCAGGCCCCTG15270.11363830868689334No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15260.11356388936227847No Hit
GGCCTGGCTTCTGCTGATACCAATAAACATACTGCTTTGACAATGCATCTCCAGAGCAGGTGATCCTGGCCGT15090.11229876084382583No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC14960.11133130962383264No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGG14960.11133130962383264No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14930.11110805164998806No Hit
CAGGGATCCCTGAGCGATTTTCTGGCTCCGGCTCAGGGACAACAGTCACGTTGACCATCAGTGGAGTCCAGGC14850.11051269705306917No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT14710.1094708265084611No Hit
GCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCA14310.10649405352386665No Hit
GGACTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCCGGAGCCAGAAAATCGCTCAGGGATCCCTGAGGGC14200.10567544095310318No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAG14140.105228925005414No Hit
GCCTCGCACTGTCTTCATATAACATCAGCACAGGGGCCTGGCCTGGCTTC14130.10515450568079913No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC13980.10403821581157623No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13960.10388937716234649No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC13890.10336844189004248No Hit
GGCTTCTGCTGATACCAATAAACATACTGCTTTGACAATGCATCTCCAGAGCAGGTGATCCTGGCCGTCTGTC13840.10299634526696817No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC13810.1027730872931236No Hit
CACCAGTATTGTCCGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTG13590.10113586215159663No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13570.10098702350236691No Hit
GTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATG13520.1006149268792926No Hit
CTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGG13520.1006149268792926No Hit
CTGTTGTCCCTGAGCCGGAGCCAGAAAATCGCTCAGGGATCCCTGAGGGCCTCGCACTGTCTTCATATAACATC13520.1006149268792926No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13510.10054050755467774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)