FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1290261
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTACTGTCAGCAGTATACTGATTCAGCCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAGAGA54100.41929501085439297No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51780.40131415271793847No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT47630.36915011768936673No Hit
GTAATATACTGCGAAATCTTCAGGCTCCAGGCTGCTGATGGTAAGAGTGAAGTCTGTCCCAGACCCACTGCC38170.2958316185639959No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC35530.2753706420638925No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32600.2526620582967322No Hit
ATATTACTGTCAGCAGTATACTGATTCAGCCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAGAGA31550.24452416991600925No Hit
GTATACTGCTGACAGTAATATACTGCGAAATCTTCAGGCTCCAGGCTGCTGATGGTAAGAGTGAAGTCTGTCCC31450.24374913292736894No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG28920.22414069711476978No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT27610.2139877125635821No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT26810.20778741665445985No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26160.20274967622829798No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG26030.20174212814306564No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA25190.19523181743848728No Hit
CTCCTGCAGGGCCAGTCAGACTGTTAGCGGCGACTACGTAGCCTGGTACC25130.19476679524530308No Hit
CAGTAATATACTGCGAAATCTTCAGGCTCCAGGCTGCTGATGGTAAGAGTGAAGTCTGTCCCAGACCCACTGCC24390.189031521529365No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG23700.18368376630774702No Hit
GCAGTATACTGATTCAGCCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAGAGAACTGTGGCTGCACC23490.18205618863160244No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23190.1797310776656816No Hit
GTCTGGGACAGACTTCACTCTTACCATCAGCAGCCTGGAGCCTGAAGATT22780.17655342601225643No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTAGCGG22420.1737632928531514No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCAGGGAGGCCAGTGGCCCTGGT22410.1736857891542874No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTAGCGGCG21900.16973310051222196No Hit
CATTTATGGTGCATCCACCAGGGCCACTGGCCTCCCTGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTC21620.16756299694402915No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA20750.1608201751428587No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGA20530.15911509376785007No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC19990.15492989402919255No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19850.15384484224509615No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC19700.15268228676213574No Hit
GTATACTGATTCAGCCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAGAGAACTGTGGCTGCACCAT19640.15221726456895154No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19380.15020216839848682No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC18900.14648199085301347No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC17950.13911913946093077No Hit
TCAGTATACTGCTGACAGTAATATACTGCGAAATCTTCAGGCTCCAGGCTGCTGATGGTAAGAGTGAAGTCTGTC17160.13299634725067253No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCAGGGAGGCCA16670.12919866600633517No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC16450.12749358463132654No Hit
CCATAAATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGCTACGTAGTCGCCGCTAACAGTCTG16030.12423842927903733No Hit
GACTTCACTCTTACCATCAGCAGCCTGGAGCCTGAAGATTTCGCAGTATATTACTGTCAGCAGTATACTGATTC15830.12268835530175677No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC15830.12268835530175677No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15660.12137079242106828No Hit
GTAATACAGGGCCGTGTCCTCGGCTCGCAGGCTGTTCATCTGCAGATACACCGTGTTCTTGGCGTTGTCTCTGG15630.12113828132447621No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15490.12005322954037982No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCTCTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGGGCTGA14980.11610054089831437No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14720.11408544472784964No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC14400.11160532636420074No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14350.11121780786988059No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14330.11106280047215253No Hit
CACCAGGGCCACTGGCCTCCCTGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTTACCATCAGC14260.11052027458010433No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14240.11036526718237627No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14000.1085051784096396No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT13860.1074201266255432No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT13850.10734262292667916No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCC13590.10532752675621443No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13170.10207237140392525No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC13060.10121983071642093No Hit
GCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGCATCCACCAGGGCCACTGGCCTC13050.10114232701755692No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC13000.10075480852323676No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAGAGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG12950.10036729002891663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)