FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1290261
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51940.4025542118997629No Hit
GTATATTACTGTCAGCAGTATACTGATTCAGCCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAGAGA48470.3756604283939451No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT40350.31272742491635414No Hit
GTAATATACTGCGAAATCTTCAGGCTCCAGGCTGCTGATGGTAAGAGTGAAGTCTGTCCCAGACCCACTGCC35760.27715322713776513No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC34390.2665352203933933No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31000.24026146647848767No Hit
GTATACTGCTGACAGTAATATACTGCGAAATCTTCAGGCTCCAGGCTGCTGATGGTAAGAGTGAAGTCTGTCCC30290.2347587038591417No Hit
ATATTACTGTCAGCAGTATACTGATTCAGCCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAGAGA28970.22452821560908992No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27340.21189511269425332No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA26540.20569481678513107No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG25950.2011220985521534No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT25360.19654938031917574No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT24630.19089161030210167No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23440.1816686701372823No Hit
CTCCTGCAGGGCCAGTCAGACTGTTAGCGGCGACTACGTAGCCTGGTACC23410.18143615904069021No Hit
CAGTAATATACTGCGAAATCTTCAGGCTCCAGGCTGCTGATGGTAAGAGTGAAGTCTGTCCCAGACCCACTGCC23060.17872352958044924No Hit
GTCTGGGACAGACTTCACTCTTACCATCAGCAGCCTGGAGCCTGAAGATT22450.1739958039497435No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG21780.1688030561258536No Hit
GCAGTATACTGATTCAGCCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAGAGAACTGTGGCTGCACC21730.16841553763153347No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCAGGGAGGCCAGTGGCCCTGGT21260.16477286378492412No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20400.1581075456826177No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTAGCGG20190.1564799680064731No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC19850.15384484224509615No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTAGCGGCG19780.15330231635304795No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGA19620.15206225717122349No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19550.1515197312791753No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC18870.14624947975642139No Hit
CATTTATGGTGCATCCACCAGGGCCACTGGCCTCCCTGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTC18740.145241931671189No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG18270.1415992578245797No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC18260.14152175412571566No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17910.13880912466547468No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC17670.136949035892738No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA17190.13322885834726464No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCAGGGAGGCCA17130.13276383615408047No Hit
GTATACTGATTCAGCCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAGAGAACTGTGGCTGCACCAT17120.1326863324552164No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16470.1276485920290546No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC16180.12540098476199776No Hit
TCAGTATACTGCTGACAGTAATATACTGCGAAATCTTCAGGCTCCAGGCTGCTGATGGTAAGAGTGAAGTCTGTC15630.12113828132447621No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15590.1208282665290201No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT15230.11803813336991509No Hit
CCATAAATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGCTACGTAGTCGCCGCTAACAGTCTG14980.11610054089831437No Hit
GACTTCACTCTTACCATCAGCAGCCTGGAGCCTGAAGATTTCGCAGTATATTACTGTCAGCAGTATACTGATTC14850.115092992813082No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14620.11331040773920936No Hit
GTAATACAGGGCCGTGTCCTCGGCTCGCAGGCTGTTCATCTGCAGATACACCGTGTTCTTGGCGTTGTCTCTGG14590.11307789664261726No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCC14450.11199284485852087No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13930.1079626525175914No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13840.10726511922781515No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13790.10687760073349502No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC13530.10486250456303028No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13520.10478500086416624No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT13380.10369994908006985No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC13220.10245988989824538No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)