FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1389670
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT72920.5247288924708744No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGGC70130.5046521836119366No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT66640.4795383076557744No Hit
GAGTTCACTCTGACCATCAGCAGCCTGCAGCTTGAAGATTCTGCAGTTTACTTCTGTCAGCAATATGATAAGTGG53730.38663855447696216No Hit
GTCTGGGACAGAGTTCACTCTGACCATCAGCAGCCTGCAGCTTGAAGATT52930.3808817920801341No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48750.3508027085567077No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46340.33346046183626327No Hit
CTCCCAGACTCCTCATCTACGGAACTTCCACCAGGGCCGCTGGTGTCCCA46230.33266890700669943No Hit
GTCCCAGACCCACTGGCACTGAACCTGGCTGGGACACCAGCGGCCCTGGT45430.32691214460987145No Hit
GGAGAAATAGTGCTGACACAGTCTCCAGCCACCCTCTCTGTGTCTCCAGG44910.3231702490519332No Hit
GGATGTGACCGTCAACTTAGGTTGGTACCAACAGAGACCTGGGCAGGCTCCCAGACTCCTCATCTACGGAACTTC42230.30388509502255934No Hit
GAGTGAACTCTGTCCCAGACCCACTGGCACTGAACCTGGCTGGGACACCAGCGGCCCTGGTGGAAGTTCCGT41560.2990638065152158No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG41430.2981283326257313No Hit
CTCCTGCAGGGCCAGTCAGGATGTGACCGTCAACTTAGGTTGGTACCAACAGAGACCTGGGCAGGCTCCCAGACT40370.2905006224499342No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT38200.27488540444853815No Hit
GTACCAACAGAGACCTGGGCAGGCTCCCAGACTCCTCATCTACGGAACTTCCACCAGGGCCGCTGGTGTCCCAGC37880.27258269948980696No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG37410.2692006015816705No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA36610.2634438391848425No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36480.2625083652953579No Hit
GTCTCCAGCCACCCTCTCTGTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGGATGTGACCGT36340.261500931875913No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA34570.24876409507293096No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG34250.2464613901141998No Hit
GTCTGGGAGCCTGCCCAGGTCTCTGTTGGTACCAACCTAAGTTGACGGTCACATCCTGACTGGCCCTGCAGGAG33760.2429353731461426No Hit
GTCAGGATGTGACCGTCAACTTAGGTTGGTACCAACAGAGACCTGGGCAGGCTCCCAGACTCCTCATCTACGGA31590.2273201551447466No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA31440.22624076219534134No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC31270.22501745018601538No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31150.2241539358264912No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGGCACTGGAGAAATAGTG31110.2238660977066498No Hit
ACAGAGACCTGGGCAGGCTCCCAGACTCCTCATCTACGGAACTTCCACCAGGGCCGCTGGTGTCCCAGCCAGGTT30810.22170731180783926No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAGGTG30810.22170731180783926No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAGGTGATCACCGGAGG29740.21400764210208179No Hit
CTGTTGGTACCAACCTAAGTTGACGGTCACATCCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCTCCTGG29670.21350392539235935No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29210.21019378701418323No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC27490.19781674786100295No Hit
GTAGTAGACGGCCGTGTCCTCGACTTTCAGGCTGCTCATTTCCAGAAAGGTTGCATTCTTGGCATTGTCTCTGG26550.19105255204473004No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25330.1822734893895673No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC24170.17392618391416667No Hit
GAAGTAAACTGCAGAATCTTCAAGCTGCAGGCTGCTGATGGTCAGAGTGAACTCTGTCCCAGACCCACTGGC24150.17378226485424597No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22850.1644275259594004No Hit
GGTCTCTGTTGGTACCAACCTAAGTTGACGGTCACATCCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCTC21920.15773528967308786No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT21740.15644001813380154No Hit
CATCCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCTCCTGGAGACACAGAGAGGGTGGCTGGAGACTGTGTC21720.15629609907388084No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21670.1559363014240791No Hit
GTGCCAGTGGGTCTGGGACAGAGTTCACTCTGACCATCAGCAGCCTGCAGCTTGAAGATTCTGCAGTTTACTT21610.15550454424431698No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21520.15485690847467384No Hit
GTTTACTTCTGTCAGCAATATGATAAGTGGCCTCCGGTGATCACCTTCGGCGGAGGGACCAAGGTGGAGATCAA21500.15471298941475314No Hit
CTGCTACTCTGGCTCCCAGATGGCACTGGAGAAATAGTGCTGACACAGTCTCCAGCCACCCTCTCTGTGTCTCC21480.15456907035483244No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC21480.15456907035483244No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21090.1517626486863788No Hit
GTTCAGTGCCAGTGGGTCTGGGACAGAGTTCACTCTGACCATCAGCAGCCTGCAGCTTGAAGATTCTGCAGTTT20990.1510430533867753No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGGCACTGGAGAAAT20940.15068325573697353No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC20720.14910014607784583No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20550.14787683406851987No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT20430.14701331970899564No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC20330.14629372440939217No Hit
CTTATCATATTGCTGACAGAAGTAAACTGCAGAATCTTCAAGCTGCAGGCTGCTGATGGTCAGAGTGAACT19340.13916973094331747No Hit
GCGCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC19200.13816229752387257No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT19160.13787445940403117No Hit
CTCTGTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGGATGTGACCGTCAACTTAGGTTGGT19130.1376585808141501No Hit
GTCCAGAGACAATGCCAAGAATGCAACCTTTCTGGAAATGAGCAGCCTGAAAGTCGAGGACACGGCCGTCTACT19120.13758662128418978No Hit
GTGCTGACACAGTCTCCAGCCACCCTCTCTGTGTCTCCAGGAGAAAGAGC19050.13708290457446734No Hit
GTCTACTACTGTGCGAGAGAAAGGTGGGGCGTCGCAGTTCCAAATATTCC18620.13398864478617226No Hit
GTCACATCCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCTCCTGGAGA18480.13298121136672736No Hit
ACTTAGGTTGGTACCAACAGAGACCTGGGCAGGCTCCCAGACTCCTCATC18460.13283729230680666No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18110.1303187087581944No Hit
GTCCCAGCCAGGTTCAGTGCCAGTGGGTCTGGGACAGAGTTCACTCTGAC18090.13017478969827367No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18050.12988695157843227No Hit
CCCTCTCTGTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGT17860.12851972050918564No Hit
GACCATCAGCAGCCTGCAGCTTGAAGATTCTGCAGTTTACTTCTGTCAGCAATATGATAAGTGGCCTCCGGTGAT17680.12722444896989932No Hit
ACCTTGGTCCCTCCGCCGAAGGTGATCACCGGAGGCCACTTATCATATTG17600.12664877273021652No Hit
GACCTGGGCAGGCTCCCAGACTCCTCATCTACGGAACTTCCACCAGGGCCGCTGGTGTCCCAGCCAGGTTCAGTG17550.12628897508041476No Hit
CCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG17370.12499370354112846No Hit
CTGCAGGAGAGGGTGGCTCTTTCTCCTGGAGACACAGAGAGGGTGGCTGGAGACTGTGTCAGCACTATTTCTCC17320.12463390589132672No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGGCACTGGAGAAATAGTGCTGACACAGTCTC17210.12384235106176286No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT17190.12369843200184218No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC16800.1208920103333885No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16690.12010045550382466No Hit
GAACCTGGCTGGGACACCAGCGGCCCTGGTGGAAGTTCCGTAGATGAGGAGTCTGGGAGCCTGCCCAGGTCT16600.11945281973418151No Hit
CTGGTGTCCCAGCCAGGTTCAGTGCCAGTGGGTCTGGGACAGAGTTCACTCTGACCATCAGCAGCCTGCAGCTTG16540.11902106255441938No Hit
CACTGGCACTGAACCTGGCTGGGACACCAGCGGCCCTGGTGGAAGTTCCGTAGATGAGGAGTCTGGGAGCCTGCC16350.11765383148517274No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA16300.11729403383537097No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16290.11722207430541064No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA16200.11657443853576749No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16180.11643051947584679No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16150.11621464088596573No Hit
CCTTGGTCCCTCCGCCGAAGGTGATCACCGGAGGCCACTTATCATATTGCTGACAGAAGTAAACTGCAGAATCTT16080.11571092417624329No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGGCACTGGAGAAATAGTGCTGACACAGTCTCCAGCCACCCT16060.11556700511632256No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15770.11348017874747243No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15550.11189706908834472No Hit
CTCCTCATCTACGGAACTTCCACCAGGGCCGCTGGTGTCCCAGCCAGGTT15540.11182510955838436No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15450.11117747378874121No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC15440.11110551425878086No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15390.11074571660897911No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC15350.11045787848913771No Hit
GCCAGGTTCAGTGCCAGTGGGTCTGGGACAGAGTTCACTCTGACCATCAGCAGCCTGCAGCTTGAAGATTCTGC15260.10981024271949455No Hit
ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGGCACTGGAGAAATAGTGCTGAC15230.1095943641296135No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15220.10952240459965315No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15190.1093065260097721No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14860.10693186152108053No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14710.10585246857167528No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTC14540.10462915656234933No Hit
GGCTGGGACACCAGCGGCCCTGGTGGAAGTTCCGTAGATGAGGAGTCTGGGAGCCTGCCCAGGTCTCTGTTGGT14010.10081530147445077No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC13990.10067138241453007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)