FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1389670
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT71790.5165974655853548No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGGC67750.48752581548137325No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT57540.4140551353918556No Hit
GAGTTCACTCTGACCATCAGCAGCCTGCAGCTTGAAGATTCTGCAGTTTACTTCTGTCAGCAATATGATAAGTGG49850.3587182568523462No Hit
GTCTGGGACAGAGTTCACTCTGACCATCAGCAGCCTGCAGCTTGAAGATT49640.35720710672317885No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46550.3349716119654306No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45330.3261925493102679No Hit
CTCCCAGACTCCTCATCTACGGAACTTCCACCAGGGCCGCTGGTGTCCCA41670.2998553613447797No Hit
GTCCCAGACCCACTGGCACTGAACCTGGCTGGGACACCAGCGGCCCTGGT40290.28992494621025133No Hit
GGAGAAATAGTGCTGACACAGTCTCCAGCCACCCTCTCTGTGTCTCCAGG40160.28898947232076677No Hit
GAGTGAACTCTGTCCCAGACCCACTGGCACTGAACCTGGCTGGGACACCAGCGGCCCTGGTGGAAGTTCCGT39960.2875502817215598No Hit
GGATGTGACCGTCAACTTAGGTTGGTACCAACAGAGACCTGGGCAGGCTCCCAGACTCCTCATCTACGGAACTTC39740.2859671720624321No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38180.2747414853886175No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA37320.2685529658120273No Hit
CTCCTGCAGGGCCAGTCAGGATGTGACCGTCAACTTAGGTTGGTACCAACAGAGACCTGGGCAGGCTCCCAGACT37220.26783337051242384No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG36750.26445127260428736No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT36180.26034957939654735No Hit
GTCTCCAGCCACCCTCTCTGTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGGATGTGACCGT35640.2564637647786885No Hit
GTACCAACAGAGACCTGGGCAGGCTCCCAGACTCCTCATCTACGGAACTTCCACCAGGGCCGCTGGTGTCCCAGC33390.24027287053760962No Hit
GTCTGGGAGCCTGCCCAGGTCTCTGTTGGTACCAACCTAAGTTGACGGTCACATCCTGACTGGCCCTGCAGGAG31660.22782387185446903No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA31410.2260248836054603No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG30890.22228298804752208No Hit
ACAGAGACCTGGGCAGGCTCCCAGACTCCTCATCTACGGAACTTCCACCAGGGCCGCTGGTGTCCCAGCCAGGTT29850.21479919693164562No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAGGTG29610.21307216821259722No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGGCACTGGAGAAATAGTG28800.20724344628580885No Hit
CTGTTGGTACCAACCTAAGTTGACGGTCACATCCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCTCCTGG28720.20666777004612605No Hit
GTCAGGATGTGACCGTCAACTTAGGTTGGTACCAACAGAGACCTGGGCAGGCTCCCAGACTCCTCATCTACGGA28600.20580425568660185No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC28440.20465290320723625No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAGGTGATCACCGGAGG28400.20436506508739485No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28380.20422114602747415No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26790.19277958076377846No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG26200.18853396849611778No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC25820.18579950635762446No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25500.18349680139889327No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC25480.18335288233897257No Hit
GTAGTAGACGGCCGTGTCCTCGACTTTCAGGCTGCTCATTTCCAGAAAGGTTGCATTCTTGGCATTGTCTCTGG24880.17903531054135155No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC24280.17471773874373053No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA23870.17176739801535618No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22990.16543495937884534No Hit
CTGCTACTCTGGCTCCCAGATGGCACTGGAGAAATAGTGCTGACACAGTCTCCAGCCACCCTCTCTGTGTCTCC22290.16039779228162082No Hit
GAAGTAAACTGCAGAATCTTCAAGCTGCAGGCTGCTGATGGTCAGAGTGAACTCTGTCCCAGACCCACTGGC22110.1591025207423345No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT21990.1582390063828103No Hit
GTTTACTTCTGTCAGCAATATGATAAGTGGCCTCCGGTGATCACCTTCGGCGGAGGGACCAAGGTGGAGATCAA21050.1514748105665374No Hit
GGTCTCTGTTGGTACCAACCTAAGTTGACGGTCACATCCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCTC21050.1514748105665374No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20980.15097109385681493No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20810.14974778184748896No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20610.14830859124828197No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGGCACTGGAGAAAT20520.1476609554786388No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20500.1475170364187181No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC20290.14600588628955077No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC20020.1440629789806213No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT19870.14298358603121605No Hit
GTGCCAGTGGGTCTGGGACAGAGTTCACTCTGACCATCAGCAGCCTGCAGCTTGAAGATTCTGCAGTTTACTT19330.13909777141335714No Hit
CATCCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCTCCTGGAGACACAGAGAGGGTGGCTGGAGACTGTGTC19120.13758662128418978No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19030.1369389855145466No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC19020.13686702598458628No Hit
GTTCAGTGCCAGTGGGTCTGGGACAGAGTTCACTCTGACCATCAGCAGCCTGCAGCTTGAAGATTCTGCAGTTT18900.13600351162506205No Hit
CTTATCATATTGCTGACAGAAGTAAACTGCAGAATCTTCAAGCTGCAGGCTGCTGATGGTCAGAGTGAACT18890.13593155209510172No Hit
CTCTGTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGGATGTGACCGTCAACTTAGGTTGGT18690.13449236149589472No Hit
GTGCTGACACAGTCTCCAGCCACCCTCTCTGTGTCTCCAGGAGAAAGAGC18570.13362884713637052No Hit
GCGCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC17840.12837580144926494No Hit
GTCCAGAGACAATGCCAAGAATGCAACCTTTCTGGAAATGAGCAGCCTGAAAGTCGAGGACACGGCCGTCTACT17820.12823188238934424No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17700.12736836802982002No Hit
GTCACATCCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCTCCTGGAGA17460.12564133931077162No Hit
CCCTCTCTGTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGT17460.12564133931077162No Hit
CTGCAGGAGAGGGTGGCTCTTTCTCCTGGAGACACAGAGAGGGTGGCTGGAGACTGTGTCAGCACTATTTCTCC17070.12283491764231795No Hit
GTCCCAGCCAGGTTCAGTGCCAGTGGGTCTGGGACAGAGTTCACTCTGAC17070.12283491764231795No Hit
GTCTACTACTGTGCGAGAGAAAGGTGGGGCGTCGCAGTTCCAAATATTCC16940.1218994437528334No Hit
GGCTGGGACACCAGCGGCCCTGGTGGAAGTTCCGTAGATGAGGAGTCTGGGAGCCTGCCCAGGTCTCTGTTGGT16640.1197406578540229No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16580.11930890067426081No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16550.11909302208437975No Hit
GACCATCAGCAGCCTGCAGCTTGAAGATTCTGCAGTTTACTTCTGTCAGCAATATGATAAGTGGCCTCCGGTGAT16550.11909302208437975No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGGCACTGGAGAAATAGTGCTGACACAGTCTC16260.11700619571552957No Hit
ACTTAGGTTGGTACCAACAGAGACCTGGGCAGGCTCCCAGACTCCTCATC16160.11628660041592609No Hit
ACCTTGGTCCCTCCGCCGAAGGTGATCACCGGAGGCCACTTATCATATTG16110.11592680276612433No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA16090.11578288370620363No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC16080.11571092417624329No Hit
GACCTGGGCAGGCTCCCAGACTCCTCATCTACGGAACTTCCACCAGGGCCGCTGGTGTCCCAGCCAGGTTCAGTG15880.11427173357703628No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15630.1124727453280275No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC15610.11232882626810682No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15580.11211294767822576No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15460.11124943331870156No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGGCACTGGAGAAATAGTGCTGACACAGTCTCCAGCCACCCT15380.11067375707901878No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15340.11038591895917735No Hit
CCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG15270.10988220224945491No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15210.1094504450696928No Hit
CTCCTCATCTACGGAACTTCCACCAGGGCCGCTGGTGTCCCAGCCAGGTT15200.10937848553973245No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15170.1091626069498514No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG15090.1085869307101686No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14950.10757949729072369No Hit
ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGGCACTGGAGAAATAGTGCTGAC14900.10721969964092196No Hit
CCTTGGTCCCTCCGCCGAAGGTGATCACCGGAGGCCACTTATCATATTGCTGACAGAAGTAAACTGCAGAATCTT14680.10563658998179425No Hit
GAACCTGGCTGGGACACCAGCGGCCCTGGTGGAAGTTCCGTAGATGAGGAGTCTGGGAGCCTGCCCAGGTCT14600.10506091374211143No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14540.10462915656234933No Hit
CTGGTGTCCCAGCCAGGTTCAGTGCCAGTGGGTCTGGGACAGAGTTCACTCTGACCATCAGCAGCCTGCAGCTTG14500.10434131844250794No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC14350.10326192549310269No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC14310.10297408737326129No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14280.10275820878338023No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATGGCACTGGAGAAATAGTGCTGACACAGTCTCCAGCCACCCTCT14150.10182273489389568No Hit
GCCAGGTTCAGTGCCAGTGGGTCTGGGACAGAGTTCACTCTGACCATCAGCAGCCTGCAGCTTGAAGATTCTGC14010.10081530147445077No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA13980.10059942288456972No Hit
CACTGGCACTGAACCTGGCTGGGACACCAGCGGCCCTGGTGGAAGTTCCGTAGATGAGGAGTCTGGGAGCCTGCC13910.10009570617484727No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)