FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166615
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT55400.47487817317624065No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49090.4207900635599577No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT37420.3207570620984644No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35820.30704216901034187No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA34990.29992756822087835No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG34240.29349871208582096No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30110.25809714430210484No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG29700.2545827029482734No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC29660.25423983062107036No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA29360.2516682881670474No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC29050.24901102763122368No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28150.24129640026915475No Hit
GGCCAAGACTTTCGCTTTCACCTTCGGCGGAGGGACGAAGGTGGCGCTCAAACGAACTGTGGCTGCACCAT27720.23761052275172184No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26790.22963874114425067No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC25680.2201240340643657No Hit
GTGTGAAATCTGTCCCAGATCCACTGCCGGAGAACCTTGATGGGACCCCA25370.21746677352854196No Hit
GATTTCACACTCACCATCAGCAGCCTGCAGCCTGAGGATGTTGCAACTTA25330.21712390120133893No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24260.207952066448657No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG24250.20786634836685625No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22370.19175134898831234No Hit
GTCTTGGCCTGTTGACAATAATAAGTTGCAACATCCTCAGGCTGCAGGCTGCTGATGGTGAGTGTGAAATCTGTC22070.18917980653428937No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC21720.1861796736712626No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21680.18583680134405953No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC21610.18523677477145417No Hit
GTGCAGCCACAGTTCGTTTGAGCGCCACCTTCGTCCCTCCGCCGAAGGTGAAAGCGAAAGTCTTGGCCTGTTGAC20540.17606494001877226No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCGCCACCTTCGTCCCTCCGCCGAAGGTGAAAGCGAAAGT20530.1759792219369715No Hit
GTATCAGCACAAACCGGGAAAAGCCCCTAGACTCCTAATTTATGGTTCATCCAGTTTGCAAAGTGGGGTCCCATC19400.166293078693485No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT18920.16217861076704826No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18830.16140714803084136No Hit
GTCTAGGGGCTTTTCCCGGTTTGTGCTGATACCAGGCAAGCCAAGTGTTA18760.160807121458236No Hit
CACCATCAGCAGCCTGCAGCCTGAGGATGTTGCAACTTATTATTGTCAACAGGCCAAGACTTTCGCTTTCACCTT18600.15943563214942377No Hit
GGTTATTAACACTTGGCTTGCCTGGTATCAGCACAAACCGGGAAAAGCCCCTAGACTCCTAATTTATGGTTCATC18220.15617834504099468No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17940.15377823875057325No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC17890.15334964834156942No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17890.15334964834156942No Hit
GTGCTGATACCAGGCAAGCCAAGTGTTAATAACCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCCAC17640.15120669629655029No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17620.15103526013294874No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17320.14846371767892577No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC17280.1481208453517227No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA17250.14786369110632042No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17210.14752081877911738No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16750.14357778701628215No Hit
GCCTGAGGATGTTGCAACTTATTATTGTCAACAGGCCAAGACTTTCGCTT16620.14246345195287222No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16540.14177770729846606No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16390.14049193607145458No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC16070.1377489574538301No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT16010.1372346489630255No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15880.13612031389961557No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT15220.13046292050076502No Hit
GTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGTTATTAACACTTGGCTTGCCTGGTATCAGCACAA15170.1300343300917612No Hit
CTCCCTCTTCCGTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGT15040.12891999502835125No Hit
TCCTCAGGCTGCAGGCTGCTGATGGTGAGTGTGAAATCTGTCCCAGATCCACTGCCGGAGAACCTTGATGGGACC15000.12857712270114818No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14770.1266056068197306No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC14770.1266056068197306No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGTTTCACGTGGCATGTCACGGA14440.12377691012030533No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14230.12197683040248924No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14220.12189111232068849No Hit
GCTGATGGTGAGTGTGAAATCTGTCCCAGATCCACTGCCGGAGAACCTTG14080.12069105917547776No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT14030.12026246876647394No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13950.11957672411206782No Hit
CCCCTAGACTCCTAATTTATGGTTCATCCAGTTTGCAAAGTGGGGTCCCA13890.11906241562126321No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13890.11906241562126321No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC13840.1186338252122594No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC13780.1181195167214548No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13740.11777664439425176No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC13710.11751949014884946No Hit
ATTGTCAACAGGCCAAGACTTTCGCTTTCACCTTCGGCGGAGGGACGAAGGTGGCGCTCAAACGAACTGTGGCTG13550.11614800084003721No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13530.11597656467643568No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCCAGATG13510.11580512851283413No Hit
GTAATAAATGGCCGTGTCCTCGGCTCTCAGGCTCTCCATTTGCAGATACAGAGAGTTCTTGGCGTTGTCTCTGG13470.11546225618563108No Hit
GTTAATAACCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCCACAG13390.11477651153122495No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG13330.11426220304042037No Hit
GTGTTAATAACCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCCACAGATGCAGACACGGAAGAGGG13290.11391933071321729No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC13220.11331930414061195No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13040.11177637866819816No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGAACCATAAATTAGGAGTCTAGGGGCTTTTCCCGGTTTGTG13000.11143350634099511No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGG12850.11014773511398362No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12790.10963342662317903No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTC12590.10791906498716372No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12510.10723332033275759No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTC12350.10586183102394534No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12160.10423318746973079No Hit
GTGGGGTCCCATCAAGGTTCTCCGGCAGTGGATCTGGGACAGATTTCACACTCACCATCAGCAGCCTGCAGCCTG12110.10380459706072698No Hit
CATCAAGGTTCTCCGGCAGTGGATCTGGGACAGATTTCACACTCACCATCAGCAGCCTGCAGCCTGAGGATGTTG12040.10320457048812162No Hit
CCATCAGCAGCCTGCAGCCTGAGGATGTTGCAACTTATTATTGTCAACAG11890.10191879926111014No Hit
GTCCCAGATCCACTGCCGGAGAACCTTGATGGGACCCCACTTTGCAAACTGGATGAACCATAAATTAGGAGTCT11710.10037587378869635No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11680.10011871954329406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)