FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166615
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT47870.41033245758026426No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45620.391045889175092No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35460.3039563180655143No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA35380.3032705734111082No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT32860.28166961679731534No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG31100.26658323440038056No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC28840.24721094791340759No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC27730.23769624083352262No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26850.23015304963505526No Hit
GGCCAAGACTTTCGCTTTCACCTTCGGCGGAGGGACGAAGGTGGCGCTCAAACGAACTGTGGCTGCACCAT26440.2266386082812239No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26230.2248385285634078No Hit
GTGTGAAATCTGTCCCAGATCCACTGCCGGAGAACCTTGATGGGACCCCA26090.2236384754181971No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25960.22252414035478715No Hit
GATTTCACACTCACCATCAGCAGCCTGCAGCCTGAGGATGTTGCAACTTA24940.21378089601110906No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA24750.21215225245689454No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC24260.207952066448657No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC24220.20760919412145395No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG23410.20066602949559195No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22160.18995126927049627No Hit
GTCTTGGCCTGTTGACAATAATAAGTTGCAACATCCTCAGGCTGCAGGCTGCTGATGGTGAGTGTGAAATCTGTC22080.18926552461609014No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG21910.1878083172254771No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21560.18480818436245033No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20600.17657924850957685No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC20270.1737505518101516No Hit
GTGCAGCCACAGTTCGTTTGAGCGCCACCTTCGTCCCTCCGCCGAAGGTGAAAGCGAAAGTCTTGGCCTGTTGAC19930.17083613702892556No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19680.16869318498390642No Hit
GTATCAGCACAAACCGGGAAAAGCCCCTAGACTCCTAATTTATGGTTCATCCAGTTTGCAAAGTGGGGTCCCATC19530.16740741375689494No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19510.16723597759329342No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC18820.16132142994904058No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18800.16114999378543907No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT18490.15849273324961533No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCGCCACCTTCGTCCCTCCGCCGAAGGTGAAAGCGAAAGT18150.15557831846838932No Hit
GTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGTTATTAACACTTGGCTTGCCTGGTATCAGCACAA17930.15369252066877248No Hit
GGTTATTAACACTTGGCTTGCCTGGTATCAGCACAAACCGGGAAAAGCCCCTAGACTCCTAATTTATGGTTCATC17890.15334964834156942No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17820.15274962176896406No Hit
CACCATCAGCAGCCTGCAGCCTGAGGATGTTGCAACTTATTATTGTCAACAGGCCAAGACTTTCGCTTTCACCTT17650.15129241437835103No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC17330.14854943576072654No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17080.14640648371570741No Hit
GTCTAGGGGCTTTTCCCGGTTTGTGCTGATACCAGGCAAGCCAAGTGTTA16960.14537786673409822No Hit
GTGCTGATACCAGGCAAGCCAAGTGTTAATAACCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCCAC16840.14434924975248903No Hit
GCCTGAGGATGTTGCAACTTATTATTGTCAACAGGCCAAGACTTTCGCTT16560.1419491434620676No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC16170.13860613827183774No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15920.1364631862268186No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15730.13483454267260409No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT15530.13312018103658876No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15380.13183440980957728No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15350.131577255564175No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15210.13037720241896425No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG15110.12952002160095663No Hit
GTGGGGTCCCATCAAGGTTCTCCGGCAGTGGATCTGGGACAGATTTCACACTCACCATCAGCAGCCTGCAGCCTG15090.12934858543735508No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA15010.12866284078294896No Hit
TCCTCAGGCTGCAGGCTGCTGATGGTGAGTGTGAAATCTGTCCCAGATCCACTGCCGGAGAACCTTGATGGGACC14470.12403406436570762No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14380.12326260162950073No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14330.12283401122049692No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14320.12274829313869615No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT14310.12266257505689539No Hit
CCCCTAGACTCCTAATTTATGGTTCATCCAGTTTGCAAAGTGGGGTCCCA14240.12206254848429002No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC14220.12189111232068849No Hit
CTCCCTCTTCCGTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGT14210.12180539423888773No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14020.12017675068467318No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC13920.11931956986666553No Hit
GTGTTAATAACCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCCACAGATGCAGACACGGAAGAGGG13900.119148133703064No Hit
GCTGATGGTGAGTGTGAAATCTGTCCCAGATCCACTGCCGGAGAACCTTG13880.11897669753946245No Hit
ATTGTCAACAGGCCAAGACTTTCGCTTTCACCTTCGGCGGAGGGACGAAGGTGGCGCTCAAACGAACTGTGGCTG13690.1173480539852479No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC13330.11426220304042037No Hit
GTTAATAACCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCCACAG13300.11400504879501806No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC13240.11349074030421347No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC13010.11151922442279587No Hit
GTAATAAATGGCCGTGTCCTCGGCTCTCAGGCTCTCCATTTGCAGATACAGAGAGTTCTTGGCGTTGTCTCTGG12900.11057632552298743No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12710.1089476819687729No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGAACCATAAATTAGGAGTCTAGGGGCTTTTCCCGGTTTGTG12690.10877624580517137No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCCAGATG12440.10663329376015224No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGTTTCACGTGGCATGTCACGGA12260.10509036828773846No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12170.10431890555153156No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12130.1039760332243285No Hit
GTCCCAGATCCACTGCCGGAGAACCTTGATGGGACCCCACTTTGCAAACTGGATGAACCATAAATTAGGAGTCT12000.10286169816091856No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC11990.1027759800791178No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGG11930.10226167158831319No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11830.10140449077030556No Hit
CATCAAGGTTCTCCGGCAGTGGATCTGGGACAGATTTCACACTCACCATCAGCAGCCTGCAGCCTGAGGATGTTG11790.10106161844310249No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT11780.1009759003613017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)