FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004717565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278538
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGTGTCCTCGGCGCCCAGGCTGTTCATTTCCAGATACAGGGTGTTCATGGAATTGTCTCTGG14490.520216272106499No Hit
GGACTGAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCC8090.29044510982343524No Hit
ATATTACTGTGCGAGAGGGGGCTATTTTGATCTTTTGAGTGGTTTTCATCTTATGGACGTCTGGGGCCAAGGGAC7640.2742893249754073No Hit
CAGTAATACTGGTCGTCTCACATACTACGCAGACTCCGTGAAGGGCCGGT7580.27213522032900356No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACGGTGCTGCTACTTGTATATGAGCTGCAGTAATAAT7500.2692630808004653No Hit
CTATATTACTGTGCGAGAGGGGGCTATTTTGATCTTTTGAGTGGTTTTCATCTTATGGACGTCTGGGGCCAAGGG7130.2559794354809756No Hit
CAGTAATATAGGGCCGTGTCCTCGGCGCCCAGGCTGTTCATTTCCAGATA6250.22438590066705438No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT5970.21433341231717035No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT5950.2136153774350358No Hit
GATTATTACTGCAGCTCATATACAAGTAGCAGCACCGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGT5640.20248583676194992No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC5590.20069074955661345No Hit
GTATTACTGATACCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATCGCTGCTA5510.1978186100280752No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC5300.19027924376566213No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA5210.18704808679605656No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACGGTGCTGCTACTTGTATATGAGCTG4990.17914970309257625No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCTCCTG4890.17555952868190336No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT4580.16442998800881747No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT4560.1637119531266829No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTCGTTATAACTATGTCTCCTGGTTCCAACAACGCCCAGGCAAAGC4540.1629939182445483No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC4510.16191686592134646No Hit
CCCCTAAGGGCCGATGAATGACATCATAAATCATGAGTGTGGGGGCTTTGCCTGGGCGTTGTTGGAACCAGG4450.15976276127494274No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAG4400.1579676740696063No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATC4390.157608656628539No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT4390.157608656628539No Hit
ATATAGGGCCGTGTCCTCGGCGCCCAGGCTGTTCATTTCCAGATACAGGG4370.15689062174640445No Hit
TAGTTATAACGACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAT4360.15653160430533716No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA4330.1554545519821353No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC4270.15330044733573156No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG4270.15330044733573156No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTCGTTATAACTATGTCTCCTGGTTCCAACAACG4240.1522233950125297No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT4130.14827420316078954No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC4000.14360697642691483No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC3990.14324795898584755No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG3970.14252992410371296No Hit
TTATAACTATGTCTCCTGGTTCCAACAACGCCCAGGCAAAGCCCCCACAC3970.14252992410371296No Hit
GGCAAAGCCCCCACACTCATGATTTATGATGTCATTCATCGGCCCTTAGGGGTTTCTAACCGCTTCTCTGGCTCC3730.13391350551809805No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC3670.13175940087169435No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG3660.13140038343062704No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT3640.13068234854849248No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA3640.13068234854849248No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC3630.1303233311074252No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC3610.12960529622529063No Hit
GACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCT3590.12888726134315603No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC3560.1278102090199542No Hit
GAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT3530.12673315669675234No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG3520.12637413925568503No Hit
GATCAGGACTGAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGC3490.1252970869324832No Hit
GCCCTATATTACTGTGCGAGAGGGGGCTATTTTGATCTTTTGAGTGGTTTTCATCTTATGGACGTCTGGGGCCA3490.1252970869324832No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCAC3370.12098887763967574No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG3370.12098887763967574No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAAC3360.12062986019860844No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG3320.11919379043433931No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAAGATGAAAAC3280.11775772067007015No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC3140.11273147649512813No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT3120.11201344161299356No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG3100.11129540673085898No Hit
CTACGCAGACTCCGTGAAGGGCCGGTTCACCATGTCCAGAGACAATTCCATGAACACCCTGTATCTGGAAATGA3060.10985933696658982No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG3050.10950031952552254No Hit
GTATGTGAGACGACCAGTATTACTGATACCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCT3050.10950031952552254No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC3010.1080642497612534No Hit
CTCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATGTCCAGAGA3010.1080642497612534No Hit
GTGTGGGGGCTTTGCCTGGGCGTTGTTGGAACCAGGAGACATAGTTATAA3000.10770523232018611No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC2970.10662817999698426No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG2960.10626916255591698No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC2960.10626916255591698No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAACTGTTGGAGT2940.10555112767378239No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG2920.10483309279164782No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGGTTAGAAACCCCTAAGGGCCGATGAATG2920.10483309279164782No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAAGTCACCATGGAGTTTGGGCTG2890.10375604046844597No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC2890.10375604046844597No Hit
CTCCTGTGTAACTTCTGGATCCACCTTTAGCAGCGATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG2880.10339702302737867No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC2880.10339702302737867No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT2870.10303800558631139No Hit
GTGCTGCTACTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGC2850.10231997070417681No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC2850.10231997070417681No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG2810.10088390093990766No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)